[PDF] Top 20 Amino-terminal sequence, synthesis, and membrane insertion of glycoprotein B of herpes simplex virus type 1.

... Analysis of gB translated in vitro in the presence and absence of membranes showed that i gB is inserted into membranes and glycosylated cotranslationally; ii a large portion of the gB p[r] ... See full document

... residues 1 to 43 (of which residues 1 to 30 specify the signal sequence), a portion of the extracellular domain consisting of residues 596 to 726, the transmembrane domain (residues 727 to 795), and ... See full document

... WT virus (23), and all mutant strains were prepared in the NS ...NS-gEnull virus has a deletion of gE amino acids 124 to 510 in the gE ectodo- main (37), whereas rNS-gEnull virus rescues the ... See full document

... lanes 1 and 4, ...31-68 virus (not FIG. 3. Construction of recombinant baculoviruses. (A and B) The approximate locations of the herpesviral genes encoding the viral glycoproteins gH, gB, gK, and gD ... See full document

... influenza virus, lymphocytic choriomeningitis virus (LCMV), and human immunodefi- ciency virus, escape humoral or cellular immunity by mutating antigens that are immune targets (18, 43, ...Vaccinia ... See full document

... (Fig. 1). First, a new recombinant virus, ⌬ 20/gKD3prtC, was constructed by replacing the UL20 gene of the recombinant virus gKD3prtC (17) with a gene cassette expressing the EGFP gene under control ... See full document

... encode membrane proteins (7, 58), and the high relative incidence of nonsilent mutations probably reflects both stochastic effects and nonstringent requirements for amino acid sequence ...Fig. ... See full document

... full-length, membrane- associated mutant protein on the viral or cell surface due to the presence of transmembrane ...of insertion would probably render even the full-length E187i5 mutant protein more ... See full document

... Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody ... See full document

... 2396-2401 0022-538X/93/042396-06$02.00/0 Copyright © 1993, American Society for Microbiology Truncation of the Carboxy-Terminal 28 Amino Acids of Glycoprotein B Specified by Herpes Simpl[r] ... See full document

... Herpes simplex virus glycoprotein H (gH) is one of the four virion envelope proteins which are required for virus entry and for cell-cell fusion in a transient ...in membrane ... See full document

... 33 insertion mutants in gH, of which 8 expressed a conformationally abnormal molecule and failed to complement a gH-negative virus in functional ...wild- type phenotypes in both virus entry ... See full document

... HSV-1 recombinants containing chimeric gC genes composed of HSV-1 and HSV-2 sequences were used to localize neomycin resistance within the N-terminal 223 amino acids of gC-2.. This regio[r] ... See full document

... of virus travel within epidermal or neuronal cells, that gE is involved in release of virus into synaptic spaces, and that gE is required for efficient transport of virus from one cell to another ... See full document

... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document

... We used three different experimental systems to rigorously characterize the anterograde spread properties of the Us9- deleted viruses (Table 1). In the Campenot chamber system, NS-Us9null retained complete ... See full document

... to membrane vesicles—either an intrinsic sorting signal or an interaction with a protein that possesses such a ...specific amino acid se- quences (2–4, 47, 62, 64, 66, ...of amino acids and ... See full document

... inoculate virus directly into the brain or footpad use routes that bypass complement defense mechanisms present at mu- cosal surfaces (5, 7, ...between virus and ... See full document

... Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus.. Potent neutr[r] ... See full document

... With these problems in mind, we examined the vaccine potential of a fully disabled herpes simplex virus type 1 mutant that is capable of only a single round of replication, since a virus[r] ... See full document