[PDF] Top 20 Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
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Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... of HCF in stimulating the HSV lytic ...mutant HCF, HSV-1 protein synthesis and replication were significantly reduced at the nonpermissive ...mutant VP16 proteins which are defective in ...reduced ... See full document
11
Potential Role for Luman, the Cellular Homologue of Herpes Simplex Virus VP16 (α Gene trans-Inducing Factor), in Herpesvirus Latency
... between HCF and Luman. We show that in cultured cells ectopically expressed Luman is retained in the cytoplasm, where it colocalizes with Calnexin, a protein normally associated with the endoplasmic ... See full document
10
Analysis of the Interaction between the Essential Herpes Simplex Virus 1 Tegument Proteins VP16 and VP1/2
... the VP16-VP1/2 interaction during infection, HSV-1 replication was largely unaffected, and the incorporation of VP16 into mature virus particles was not ...the VP16- VP1/2 interaction is ... See full document
11
ICP0, ICP4, or VP16 Expressed from Adenovirus Vectors Induces Reactivation of Latent Herpes Simplex Virus Type 1 in Primary Cultures of Latently Infected Trigeminal Ganglion Cells
... image acquisition were performed as described above for Southern blot analysis. (iii) Western blot analysis. Vero cells were infected with 10 PFU of KOS or Ad.C-GFP/cell or were coinfected ... See full document
11
Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells
... The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive ...by cellular RNA polymerase II (RNA pol ... See full document
12
Evidence that Herpes Simplex Virus VP16 Is Required for Viral Egress Downstream of the Initial Envelopment Event
... expected, cells infected with 8MA displayed a severe reduction in the rate of viral and cellular protein syn- thesis at 9 and 12 h ...blot analysis, which re- vealed a ⬃800-nucleotide deletion ... See full document
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Control of VP16 Translation by the Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27
... HSV-infected cells and thereby influence the translational ef- ficiencies of either viral or cellular ...of infected cells containing ICP27 show defective splicing in vitro (1, 20, ... See full document
12
Protein interactions in the herpes simplex virus type 1 VP16-induced complex: VP16 peptide inhibition and mutational analysis of host cell factor requirements.
... the cellular target of REHAY-based peptides and, by extension, of the corresponding region of VP16 in VIC assembly, was HCF (13, 14, ...overlapping VP16 peptide (contain- ing residues 360 to ... See full document
9
Characterization of VP22 in Herpes Simplex Virus-Infected Cells
... the herpes simplex virus (HSV) tegument protein VP22 by gel filtration, glycerol sedimentation, and chemical cross-linking experiments and use time course radiolabeling and immunoprecipitation assays ... See full document
14
Stabilization but Not the Transcriptional Activity of Herpes Simplex Virus VP16-Induced Complexes Is Evolutionarily Conserved among HCF Family Members
... not HCF-2, can promote VP16- induced transcription ...three HCF pro- teins can interact with VP16 and stabilize the VP16-induced complex, we next asked whether the VP16-induced ... See full document
10
Herpes Simplex Virus Transactivator VP16 Discriminates between HCF-1 and a Novel Family Member, HCF-2
... additional cellular HBM-contain- ing transcription factors, analogous to LZIP, that preferen- tially associate with HCF-2 rather than ...an HCF molecule to a site-specific activator is not fully ... See full document
11
Localization and putative function of the UL20 membrane protein in cells infected with herpes simplex virus 1.
... With the aid of this antiserum and an antibody to a constituent of the Golgi aparatus, we determined the following: i the UL20 gene is regulated as a -Yl gene, and the protein can be det[r] ... See full document
12
Exploring the Nuclear Envelope of Herpes Simplex Virus 1-Infected Cells by High-Resolution Microscopy
... mock-infected cells grown in cell culture flasks were trypsinized and centrifuged at 150 ⫻ g for 8 ...until cells were ...sedimented cells, sandwiched between two flat aluminum specimen ... See full document
12
Redistribution of microtubules and Golgi apparatus in herpes simplex virus-infected cells and their role in viral exocytosis.
... for cells undergoing mitosis (13, 14) and for cells exposed to agents like nocodazole and colchicine, which bind tubulin heterodimers and inhibit their polymerization and thus induce progressive ... See full document
11
Effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus.
... From these results, we conclude that ara-C has little effect upon early HSV-1 protein synthesis but causes a rapid drop of amino acid incorporation into late HSV-1 proteins which begins [r] ... See full document
10
Lifetimes of mRNA molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells.
... In addition, when the viral proteins made after the addition of actinomycin were further analyzed by gel electrophoresis, it was found that messengers for individual viral proteins trans[r] ... See full document
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Antibody-Dependent Cellular Cytotoxicity to Target Cells Infected With Herpes Simplex Viruses: Functional Adequacy in the Neonate
... activity of cytotoxic effector cells in cord blood, and (2) the ability of the maternal serum antibody.. mediating the ADGG reaction to pass the pla- centa.[r] ... See full document
9
Deoxyribonucleic Acid Synthesis in Synchronized Mammalian KB Cells Infected with Herpes Simplex Virus
... 783-791 Copyright © 1971 American Society for Microbiology Deoxyribonucleic Acid Synthesis in Synchronized Mammalian KB Cells Infected with Herpes Simplex Virus Received for publication [r] ... See full document
9
Aanlysis of dCMP deaminase and CDP reductase levels in hamster cells infected by herpes simplex virus.
... For this purpose, mutant Chinese hamster [A1 cells devoid of dCMP deaminase activity or Syrian hamster BHK21/C13 cells were infected by HSV type 1 or 2, and the activities of thymidine k[r] ... See full document
7
Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1
... of infected DC indicate that the cytopathicity of HSV infection of immature MDDC is slow despite the high proportion of infected ...that infected DC may continue to function for the first and ... See full document
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