[PDF] Top 20 Analysis of Mutations in the Yeast mRNA Decapping Enzyme
Has 10000 "Analysis of Mutations in the Yeast mRNA Decapping Enzyme" found on our website. Below are the top 20 most common "Analysis of Mutations in the Yeast mRNA Decapping Enzyme".
Analysis of Mutations in the Yeast mRNA Decapping Enzyme
... MFA2pG mRNA decay tion phenotype in vivo, while the other two (dcp1-41 and at 188 (Figure 3), but they showed no effect on CYH2 dcp1-42) were defective roughly to the same extent as premRNA accumulation at this ... See full document
14
Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations.
... WTm), then viability is restored. Together these results indicate that both the number and position of different phosphorylation sites are im- portant for CTD function. The pr[r] ... See full document
11
ANALYSIS OF MEIOSIS-DEFECTIVE MUTATIONS IN YEAST BY PHYSICAL MONITORING OF RECOMBINATION
... Despite these apparent differences, both our analysis of LEU2 prototrophs and the examination of HIS1 prototrophs (GAME et al. 1980) showed that a significant fract[r] ... See full document
17
High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes
... excess mutations that may affect other ...of mutations resulting in a higher density in the popula- ...of mutations in the target genes by screening a realistic number of mutagenised ... See full document
15
Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme.
... virus mRNA capping enzyme is an autonomous bifunctional domain with triphosphatase and guanylyltransferase ...cluster mutations, R77 to A (R77A)-K79A and E192A-E194A, which selectively inacti- vated ... See full document
7
Structure-Function Analysis of Yeast mRNA Cap Methyltransferase and High-Copy Suppression of Conditional Mutants by AdoMet Synthase and the Ubiquitin Conjugating Enzyme Cdc34p
... methyltransferase mutations by exogenous AdoMet: The simplest explanation for sup- pression of abd1 mutations by overexpression of AdoMet synthase is that elevated intracellular levels of the methyl donor ... See full document
16
The Taz1p Transacylase Is Imported and Sorted into the Outer Mitochondrial Membrane via a Membrane Anchor Domain
... of mutations in the membrane anchor and flanking sequences, this study suggests that the molecular basis for BTHS in a subset of patients is caused by defects in mitochondrial targeting and local- ...detailed ... See full document
9
Two Related Proteins, Edc1p and Edc2p, Stimulate mRNA Decapping in Saccharomyces cerevisiae
... a decapping defect when combined with the et ...co-immunoprecipitate mRNA (Tharun et One possibility is that the specificity of Edc1p for Dcp1p, ...the decapping protein they interact with Our data ... See full document
11
Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein
... in yeast. A. Western blot analysis of human Pcbp2 synthesis in ...wild-type yeast; ⫹ Pcbp2, extract from yeast transformed with a plasmid encoding human Pcbp2 ...of yeast producing ... See full document
12
Mutations in the Saccharomyces cerevisiae LSM1 Gene That Affect mRNA Decapping and 3′ End Protection
... mutagenic analysis of L ...such mutations on the One complex, consisting of L sm1p through Lsm7p pro- functions of L ...of mRNA decapping (Boeck et al ... See full document
14
Mutational analysis of the RNA triphosphatase component of vaccinia virus mRNA capping enzyme.
... virus mRNA capping enzyme is a multifunctional protein with RNA triphosphatase, RNA gua- nylyltransferase, and RNA (guanine-7-) methyltransferase ...The enzyme is a heterodimer of 95- and 33-kDa ... See full document
7
Site-specific selection reveals selective constraints and functionality of tumor somatic mtDNA mutations
... specific mutations, although the underlying mechanism is largely ...Further analysis indicated that these three triplet-bases were not related with the selected codons shown in ...base mutations in ... See full document
12
The role of PHD2 mutations in the pathogenesis of erythrocytosis
... of mutations (with the exception of 3 cases targeting the residue Arg371; otherwise, mutations are dispersed all through the ...of mutations in the high iron Fe (HFE) ...N203K mutations) have ... See full document
20
A Yeast-Based Genetic System for Functional Analysis of Viral mRNA Capping Enzymes
... covalent enzyme-(lysyl-N)-GMP intermediate ...the enzyme attacks the ␣ phos- phorus of GTP to form the covalent intermediate and expel ...capping enzyme (24, 59, ... See full document
9
Host Deadenylation-Dependent mRNA Decapping Factors Are Required for a Key Step in Brome Mosaic Virus RNA Replication
... and yeast methods. Standard yeast genetics and culture condi- tions were used (4, ...mutant yeast strains, including lsm1⌬, lsm6⌬, lsm7⌬, pat1 ⌬ , dhh1 ⌬ , vps16 ⌬ , edc1 ⌬ , edc2 ⌬ , upf1 ⌬ , upf2 ⌬ ... See full document
6
The D10 Decapping Enzyme of Vaccinia Virus Contributes to Decay of Cellular and Viral mRNAs and to Virulence in Mice
... In considering further in vitro and in vivo studies on the role of D10, we had concerns that the phenotype of the D10R deletion mutant might be affected by transcription of the inserted EGFP ORF regulated by a strong ... See full document
10
Characterization of a Second Vaccinia Virus mRNA-Decapping Enzyme Conserved in Poxviruses
... Plasmid construction. To create pMAL-c2x-malE-D9-his, a plasmid encod- ing a maltose binding protein (MBP)-D9 fusion protein appended with a C-terminal 10-histidine tag (MBP-D9-HIS), the D9R open reading frame was ... See full document
6
Identification of Edc3p as an Enhancer of mRNA Decapping in Saccharomyces cerevisiae
... of mRNA decay in yeast initiates with deadenylation, followed by mRNA decapping and 5⬘–3⬘ exonuclease ...the mRNA decay ...in mRNA decapping and 5⬘–3⬘ degradation ... See full document
12
Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion
... of mRNA and enzyme activity of each gene in the integrated strain were measured by qRT-PCR and spectrophotometry, respectively ...of mRNA showed decreasing trend as the distance between promoter and ... See full document
12
The mRNA Decay Pathway Regulates the Expression of the Flo11 Adhesin and Biofilm Formation in Saccharomyces cerevisiae
... The mRNA levels of another Flo11 repressor, SOK2, were not affected (with a cutoff of ...short mRNA poly(A) tails in the wild type (long/short ratio of ...NRG1 mRNA in the double ccr4Δ puf5Δ mutant ... See full document
9
Related subjects