[PDF] Top 20 Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
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Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
... QPCR analysis, Taqman (Applied Biosystems, Foster City, California), has become available which provides remarkably rapid and accurate determination of target sequences in a sample ...Taqman analysis ... See full document
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DETECTION AND DIFFERENTIATION OF HUMAN PROTOZOAN PARASITES IN STOOL SPECIMENS BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City, Egypt
... - 585 - and sensitivity of MAS-PCR in this study, we propose its application as an alternative tool in routine diagnosis and in epidemiological studies of intestinal parasites. This method will provide more accurate ... See full document
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DNA analysis by polymerase chain reaction of saliva traces on cigarette butts exposed to indoor and outdoor environmental conditions
... for analysis and its rapid degradation due to extreme effects of environmental ...butt samples were smoked, collected and were exposed to outdoors and indoors for 1 day, 3 days and 7 ...The ... See full document
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Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA
... We have compared the sensitivity and specificity of quantitative mycobacterial culture against results obtained by using the polymerase chain reaction for the detection of DNA from organ[r] ... See full document
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... for analysis by either straight needle vitreous aspiration or ideally ...benefit from PPV in terms of removal of the whole vitreous body and a higher diagnostic yield of the ... See full document
5
Measurement of bioprocess containment by quantitative polymerase chain reaction
... and environmental testing markets as well as being a powerful tool in molecular ...of bacteria in a variety of ...DNA from prokaryotes of the ecosystem. Using PCR, Chaudry and co-workers ... See full document
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Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... amplify specific parts of the ...Each reaction contained 5 µL DNA template from disc extracts, 10 µL of LightCycler 480 SYBR Green I Mas- ter (Roche diagnostics Gmbh, Germany), 1 µL of 10 µM forward ... See full document
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DETECTION OF HUMAN INTESTINAL PROTOZOA BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City
... Allele Specific Polymerase Chain Reaction (MAS-PCR) assay and microscopy for detection and identification of common pathogenic protozoan parasites in New Damietta city of North Delta region, ... See full document
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Airway bacteria measured by quantitative polymerase chain reaction and culture in patients with stable COPD: relationship with neutrophilic airway inflammation, exacerbation frequency, and lung function
... Statistical analysis was performed using PASW Statistics for Windows, Version ...performed using the Kolmogorov–Smirnov ...assessed using intraclass correlation coef- ficients (ICCs) and a ... See full document
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Original Article Clinical value of exhaled breath condensate let-7 in non-small cell lung cancer
... let-7 from 180 samples were analyzed using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR), consist- ing of 30 patients with NSCLC (lung cancer ... See full document
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LincRNA-ROR promotes metastasis and invasion of esophageal squamous cell carcinoma by regulating miR-145/FSCN1
... tissue samples and matched adjacent tissues was quantified with real-time fluorescent quantitative polymerase chain reaction ...tissues from The Cancer Genome Atlas (TCGA) ... See full document
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Ability of real time PCR for differential diagnosis of various forms of cutaneous leishmaniasis: a comparative study with histopathology
... Results: The ability of real‑time PCR for leishmania detection was higher than histopathological evaluation. The quantitative real‑time PCR (qPCR) quantified parasite loads were highly correlated with microscopic ... See full document
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Altered Expression of Circulating miR-377 and miR-98 in Relapsing-remitting Multiple Sclerosis
... to environmental factors in genetically susceptible individuals ...of analysis and a growing consensus that individual genetic variation likely influences therapeutic responses has made the search for ... See full document
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Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction
... a specific order of functions at the molecular ...gained from nanobarcod- ing has the potential to aid current NP design efforts and redefine regulatory science such that potentially life-saving NP ... See full document
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Prevalence of salmonella in food samples of dehradun region
... The exceedingly variable manifestations of typhoid fever have lead to the development of numerous diagnostic techniques. The routine detection of Salmonella in the environment including in foods and beverages is a ... See full document
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Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction
... Sequence analysis. BLAST search of the Iranian tet(A) and tet(S) sequences in GenBank showed sequence identities ranging from 99.00% to 100% for these two genes. Whereas, sequence analysis of the ... See full document
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Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection
... from active TB (ATB) patients exhibited characteristic expression under purified protein derivative (PPD) chal- lenge [16]. MiRNA expression profiles were different in PBMCs from patients with active TB, ... See full document
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Quantitative trait locus analysis of resistance to panicle blast in the rice cultivar Miyazakimochi
... map from the segregating data of the ...QTL analysis, composite interval mapping (CIM) was performed using Windows QTL Cartographer soft- ware (version ... See full document
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... Immunohistochemistry was employed for evaluation and localization of MT-1 protein expression in airway biopsy samples from the two groups. It was immediately observed that the thickness of the bronchial ... See full document
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Detection of bacteria in middle ear effusions based on the presence of allergy: does allergy augment bacterial infection in the middle ear?
... children from 3–10 years old were enrolled in this ...of bacteria detec- tion using conventional culture methods was only ...DNA using PCR was ...the bacteria did not differ ... See full document
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