[PDF] Top 20 Assembly of viruslike particles by recombinant structural proteins of adeno-associated virus type 2 in insect cells.

... Preparations of VLPs from insect cells infected with different combinations of recombinant baculoviruses showed different relative proportions of the expressed proteins.. In addition, th[r] ... See full document

... Rep proteins, DNA, and capsids should be expected. Rep proteins 78 and 52 and AAV-2 DNA showed a perfect colocalization pattern (see also reference ...capsid proteins by the B1 antibody with ... See full document

... The proteins encoded by the adeno-associated virus type 2 (AAV-2) rep and cap genes obtained during a productive infection of HeLa cells with AAV-2 and ... See full document

... A recombinant baculovirus, expressing nucleocapsid (N) protein of RVFV at high level under the control of the polyhedrin promoter was ...single recombinant virus was generated that expressed the RVFV ... See full document

... The virus stocks were pre- pared as described previously (35, 39), with the following ...293 cells with 10 m g each of the recombinant AAV plasmid and the pAAV/Ad helper plasmid per confluent ... See full document

... of adeno-associated virus type 4 ...serotypes 2 and 3, with none of the changes occurring in regions which had previously been shown to affect the known functions of Rep68 or ...capsid ... See full document

... HeLa cells at 4°C for 30 min and then raised the temperature to 37°C to initiate cell ...to cells at an MOI of ⬃10,000 particles per cell, and cells were coinfected with Ad5 at an MOI of ... See full document

... capsid proteins from both rAAV-2 and rAAV- 2cap5 were ubiquitinated in HeLa cells in the presence of LLnL ...lanes 2 and ...in cells. FIG. 3. Effects of proteasome inhibitors on ... See full document

... HCV proteins. For immunoblot analysis, Sf9 cells infected with ...(PMSF), 2 mg of aprotinin per ml, and 2 mg of leupeptin per ...infected cells was incubated with 1 ml of anti-E2(9284) ... See full document

... AAP-dependent assembly of physical particles derived from the different serotypes, we performed nondenaturing dot blotting ...viral particles to wild-type levels, congruent with the ... See full document

... subunit virus/nucleocapsid-like particles (VLPs/NLPs) has been efficiently produced in yeast that therefore has a notable value in vaccine production tech- ...Rotavirus-like particles were assembled ... See full document

... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ... See full document

... for type 2 rAAV production drove type 2 Rep72 expression with a truncated promoter for the immediate-early 1 gene of Orgyia pseudotsugata nuclear polyhedrosis virus ( ⌬ IE) and ... See full document

... and 2, but were detected in all other ...293T cells transfected with pDG and the indicated ...of cells were processed by procedure 1 with NaOH as described in Materials and ...293T cells ... See full document

... primary cells in the absence of apparent viral gene expression (61) might be due to functional Rep78 associated with the incoming AAV-2 virion ...cellular proteins involved in mediating the ... See full document

... in cells coin- fected with ...these cells, and the rAAV lacZ genome was recruited to the distinct foci within the nucleus, as was revealed by the in situ hybridization signal ...incoming recombinant ... See full document

... RESULTS Electrophoresis of the proteins of mature poliovirus particles disrupted by boiling and radioiodinated in vitro revealed the well-known pattern 14 of the four structural polypept[r] ... See full document

... These and other observations indicate that i the products of the UL18, UL19, UL35, and UL38 genes self-assemble into structures that form the outer surface icosahedral shell of the capsi[r] ... See full document

... The mechanism of AAV-2-induced differentiation is not clear, and we do not know whether cessation of cell proliferation marked by a decrease of c-myc expression, arrest of cells in the G[r] ... See full document

... endocytosed virus versus degraded virus, we performed Western blot analysis using total cell lysates of infected HeLa ...AAV5 particles negatively stained with 2% uranyl acetate in aqueous ... See full document