[PDF] Top 20 Direct Bacterial Identification in Positive Blood Cultures by Use of Two Commercial Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems
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Direct Bacterial Identification in Positive Blood Cultures by Use of Two Commercial Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems
... of blood cultures. A total of 202 available blood culture broths that were Bactec positive as indicated by the Bactec system (Bactec Plus Aerobic, Bactec F Lytic Anaerobic, and Bactec Myco ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... monomicrobial blood cul- ture isolates ...extraction systems for blood culture analysis which have reported correct identification rates ranging from 67 to 100%, with higher success rates for ... See full document
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Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... appropriate identification systems or updated databases (1, 10, 21, 22, 45, ...38). Matrix-assisted laser desorption ionization–time of flight mass ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... from direct urine samples in order to provide early diagnosis of UTIs; performing this methodology on the day of sample collection reduces the identification of the etiological agent by 18 to 24 h and ... See full document
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for ... See full document
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Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... nitrogen laser. The spectra were re- corded in the linear mode in a mass range of 2 to 20 kDa and subsequently analyzed using MALDI Biotyper Automation Control and Biotyper ...the time, the database ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... for bacterial culture are assigned to one of eight workstations (“benches”) after inoculation: blood cultures; sterile body fluid cultures; urine cultures; stool cultures; ... See full document
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... for direct analysis in the Bruker MALDI BioTyper (study identification method) using an in- house extraction ...a bacterial suspension adjusted to a McFarland standard of ...MALDI matrix (a ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... The same approach was used in our case. Reliable identifica- tion of the clinical isolate was possible only after the generation of an in-house database, expanding the stock Bruker Biotyper data- base. To our surprise, ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization
... of blood cultures using MALDI-TOF MS ...from blood culture bottles with MALDI-TOF MS reduces the TAT, ranging from ...the time to optimal treatment was also improved, with results ranging from ... See full document
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Microbial Typing by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?
... relevant bacterial species. This approach is attractive since whole-cell mass spectrometry is commonly used for species identification with potentially exploitable data already available from ... See full document
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Evaluation of a Semiquantitative Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
... McFarland standard. Furthermore, cells were isolated from the blood culture fluid using serum separator tubes instead of the Sepsityper kit. The incubation time was prolonged to 2.5 h to 3 h, depending on ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... of identification to be determined for ...the use of MALDI-TOF MS revealed the further occurrence of certain species not well known until recently, such as ...available identification systems ... See full document
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Rapid Identification of Bacteria and Yeasts from Positive Blood Culture Bottles by Using a Lysis Filtration Method and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrum Analysis with the SARAMIS Database
... a positive-blood-culture bottle after exam- ination of multiple positive bottles ...of blood processed with this method is one possible way to alleviate low cell density with some ...The ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... including blood, chocolate, inhibitory mold agar (IMH), and chromogenic me- dium did not significantly affect final identification results (28– ...level identification for ...correct ... See full document
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... any identification system, has limitations even for common clinical isolates, and the identifications obtained must be interpreted by experienced laboratory personnel in conjunction with clinical presentation and ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... Bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species ...identification. Matrix-assisted ... See full document
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Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory: Impact of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... MALDI-TOF mass spectrum ...in two independent ...by identification of 10 colonies of the same bacterial species in another independent ...MALDI-TOF mass spectrum database has 6,213 ... See full document
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Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... Phoenix systems, MicroScanWalkAway plus, Synergies plus ® , and MICroSTREPplus ® and MALDI TOF spectrometry are available for identification and antimicrobial susceptibility profiling of primary ... See full document
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Evaluation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates
... MALDI-TOF identification at the species level; none of the isolates analyzed was identified with this technique at the complex ...identified: two achieved no peaks and one yielded no reliable ... See full document
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