[PDF] Top 20 Cellular proteins which can specifically associate with simian virus 40 small t antigen.

... When similar amounts of cell extract protein z50 ,ug were applied to two portions of the BSA + t-Sepharose adsorbent, the amount of the 57K protein appearing after elution with soluble t[r] ... See full document

... Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen i[r] ... See full document

... analyze small-t-induced phenotypic progression of these cells, small-t-coexpressing cell lines were generated by transfection of established cells with small t ex- pression ... See full document

... 612-620 0022-538X/82/050612-09$02.00/0 Cellular Proteins Reactive with Monoclonal Antibodies Directed Against Simian Virus 40 T-Antigen LIONEL CRAWFORD,'* KEITH LEPPARD,1 DAVID LANE,2 AN[r] ... See full document

... To establish whether p73 is related to the hsp70 family of proteins, we attempted to immunoprecipitate the p73/T-ag complexes from cellular extracts of ST803 cells by using a monoclonal [r] ... See full document

... we can esti- mate that there are relatively few topo I molecules bound because the presence of the enzyme had no detectable effect on the mobility of the complex on our ...Other cellular proteins ... See full document

... st can induce cell cycle progression in certain scenar- ios and activate signal transduction pathways involving MEK/ ERK and JNK via PP2A binding (16, 28, 29, 47, 56, 72, 73) ...of cellular and viral ... See full document

... large T antigen, which contains 708 amino acids, plays vital roles in coordinating viral DNA repli- cation and transformation (6, 18, 47, ...of T antigen include an N- terminal J ... See full document

... To detect SV40-related proteins expressed in bacteria and compare them with authentic SV40 small t antigen, both SV40-infected monkey cells and HP1 bacterial cells were pulse-labeled wit[r] ... See full document

... genes specifically regulated by LT or the LT-p53 complex so far had been hampered by the virtual lack of bona fide cellular LT and LT-p53 target ...LT can bind SV40 DNA in a sequence-specific man- ... See full document

... To determine whether any of the human MTAPs were major cellular proteins, we analyzed Nonidet P-40 lysates of 293 cells metabolically labeled with methionine in parallel with MT immunopr[r] ... See full document

... Twenty-four hours after infection of subconfluent 1OT1/2 cells with WT SV40 100 to 200 PFU per cell, cells were fixed and stained for actin cables or for large T antigen expression.. Whi[r] ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... 623 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Monkey and mouse cells producing simian virus 40 small t antigen in the absence of clearly detectable intact or trun[r] ... See full document

... It is possible to explain this result on the basis of the nucleotide sequence: the normal splicing event of the small-t mRNA still occurs, but as the deletion shifts the reading frame, t[r] ... See full document

... MUMBY,2 AND KATHLEEN RUNDELL'* Department of Microbiology-Immunology and The Cancer Center, Northwestern University, Chicago, Illinois 60611-3008,l and Department of Pharmacology, Univer[r] ... See full document

... In a different approach to demonstrate the location of SV40 T-Ag-related molecules on the outer surface of the plasma membrane, living SV3T3 cells were treated as an intact monolayer wit[r] ... See full document

... Immunoperoxidase staining of cells infected by the simian virus 40 defective strain SV402 with PAb280 and other anti-T antibodies demonstrated that this virus produced an N-terminal frag[r] ... See full document

... To establish such a system, we infected rat Flll cells with the retroviral large T expression vector pZIPTEX 7, which directs the expression of limiting amounts of large T and thus Cellu[r] ... See full document

... The most likely candidates for such a linker are the recessive oncoproteins pRb and p107, both of which can bind cyclin A and p33cdk2 independently of viral oncoproteins 14, 23, but it i[r] ... See full document