[PDF] Top 20 Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...protein, ... See full document
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... Mutant T antigens bind to the replication origin ...require binding to the replication origin, we tested the untwisting-defective mu- tants 126PS and 135PL for the ability to bind the ...Two ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) shows ... See full document
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... N-terminal domain reduces the multimerization of the OBD under our assay ...larger anisotropy gains than N260 upon binding to nonspecific ds- and ssDNA in FP ...terminal domain stabilizes the ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... relative binding affinity of the mutant T-Ag OBDs for substrates containing ...the T-Ag OBD mutants for ssDNA was measured by fluorescence anisotropy as previously described (reference ... See full document
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... tide containing a wild-type copy of site II. Additional assays demonstrated that the 1&4 pentanucleotide double mutant is a member of a set of mutants (the inactive set of pentanucleotide double mutants [Fig. 6A]) ... See full document
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...this domain most likely begins after residue 301 and that the region between ... See full document
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... core origin and structure of SV40 large T-antigen origin binding ...(A) DNA sequence of the SV40 core ...core origin is depicted as B-form DNA, with the ... See full document
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
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Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document
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Mapping of helicase and helicase substrate-binding domains on simian virus 40 large T antigen.
... 2014 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We generated fragments of simian virus 40 large tumor antigen T antigen by tryptic digestion and assayed them for h[r] ... See full document
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Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.
... Nine monoclonal antibodies reacted with determinants located in region I, because the antibodies precipitated small t as well as large T antigens from extracts of SV40-transformed or -in[r] ... See full document
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DNA binding properties of simian virus 40 T-antigens synthesized in vivo and in vitro.
... Premixing the labeled reticulocyte cell-free products with the same amount of infected-cell extract normally used in a DNA binding experiment, representing a great excess over that synth[r] ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD which are defective for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates (i.e., the core origin [0], site I ... See full document
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Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.
... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ... See full document
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Simian Virus 40 Large T Antigen Disrupts Genome Integrity and Activates a DNA Damage Response via Bub1 Binding
... mimics DNA damage or that ATR acts upstream to phosphor- ylate ATM on Ser1981 ...cellular DNA replication dynamics, for example, rereplication or increased replication fork stall- ing, and this is sensed as ... See full document
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity.
... Second, wild-type T antigen's nonspecific DNA binding, but not its origin binding, was significantly inhibited with a number of monoclonal antibodies PAb1O4, PAb114, PAb252, PAb4O2, PAb4[r] ... See full document
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