[PDF] Top 20 Characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40.
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Characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40.
... In addition, the role of the gene A product if any in the maintenance of the transformed 1106 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Seven lines derived from p[r] ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
8
Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ... See full document
5
Temperature-Sensitive Simian Virus 40-Transformed Cells: Phenomena Accompanying Transition from the Transformed to the “Normal” State
... It may be important to note that: i the shedding of dead cells ends at a point in which the cultures have reached the density characteristic of their saturation at 39 C, and the cells re[r] ... See full document
7
Physical and Genetic Characterization of Deletion Mutants of Simian Virus 40 Constructed In Vitro
... Mutant dl-1244 was also sensitive to BamHI endonuclease cleavage and yielded a normal HindII + III-G fragment; however, its deletion at map position 0.15 was confirmed by the fact that h[r] ... See full document
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Temperature-Sensitive Mutants of Simian Virus 40: Infection of Permissive Cells
... Plaque characteristics of wild-type and mutant simian virus 40 SV40 stocks propagated at 32 C Virus Efficiency of plating39 C/32 C Time of plaque appearance in days d Infected cultures w[r] ... See full document
9
Temperature-sensitive BC mutants of simian virus 40: block in virion assembly and accumulation of capsid-chromatin complexes.
... In addition, we have tested the state of the VP1 protein produced in cells infected with selected mutants of the BC group tsBC216, tsBC223, or tsB248 and found that a stable form of this[r] ... See full document
7
Temperature-Sensitive Simian Virus 40 Mutant Defective in a Late Function
... To determine whether mutant SV40tsTNG-1 also induced cellular DNA synthesis, 5-day-old cultures of primary mouse kidney cells 3.3 X 106 cells per culture were infected at input multiplic[r] ... See full document
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Genetic Analysis of Simian Virus 40 III. Characterization of a Temperature-Sensitive Mutant Blocked at an Early Stage of Productive Infection in Monkey Cells
... The mutant virion can adsorb to and penetrate the cell normally at the restrictive temperature, but cannot induce the synthesis of cellular deoxyribonucleic acid DNA nor initiate the syn[r] ... See full document
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Temperature dependency for maintenance of transformation in mouse cells transformed by simian virus 40 tsA mutants.
... The great majority of mouse embryo fibroblast transformants generated with tsA mutant virus were temperature sensitive for transformation in all three assays, regardless of the input mul[r] ... See full document
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DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ... See full document
6
Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
... Therefore, to analyze further the mechanism of transformation, we have tried to isolate mutants of SV40 i which are temperature sensitive in their ability to transform nonpermissive rat [r] ... See full document
5
Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ... See full document
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Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
... The present study demonstrates that a molecular form of T antigen induced by ts 900, a transformation-defective ts mutant of SV40 29, is more heat labile than that induced by WT virus, i[r] ... See full document
6
Products of complementation between temperature-sensitive mutants of simian virus 40.
... These results imply that i the D product may be synthesized late in infection; ii therefore, any defect exhibited by D virions early in infection probably results from the introduction o[r] ... See full document
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Biological and biochemical studies of cells transformed by simian virus 40 temperature-sensitive gene A mutants and A mutant revertants.
... Virus recovered from both of the tsA30-transformed cell lines was temperature sensitive for viral DNA synthesis in BSC-1 cells, as is characteristic of SV40 A mutants Table 2.. That reco[r] ... See full document
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Simian virus 40 mutants carrying two temperature-sensitive mutations.
... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ... See full document
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A simian virus 40 dl884/tsA58 double mutant is temperature sensitive for abortive transformation.
... Our major conclusions are that i although the mutant tsA58 is not temperature sensitive for the maintenance of transformation, the d18841tsA58 double mutant is; ii small t antigen can pr[r] ... See full document
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Simian virus 40-associated small RNA: mapping on the simian virus 40 genome and characterization of its synthesis.
... However, within 701 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The simian virus 40 SV40-associated small RNA SAS-RNA has previously been shown to arise late in SV4[r] ... See full document
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Construction and analysis of viable deletion mutants of simian virus 40.
... The SV40 map location and size of the deletions were determined by the Si nuclease mapping procedure 18 and from the change in mobility of fragments produced by Hind II+III endonuclease [r] ... See full document
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