[PDF] Top 20 Clinical Application of PCR Restriction Enzyme Pattern Analysis for Rapid Identification of Aerobic Actinomycete Isolates
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Clinical Application of PCR Restriction Enzyme Pattern Analysis for Rapid Identification of Aerobic Actinomycete Isolates
... significant aerobic actinomycetes (9–11, ...129 isolates, respectively, have been studied by PRA, the entries in the database for slow-growing mycobacteria have been less extensively ...10 isolates ... See full document
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Nocardia veterana, a New Emerging Pathogen
... 15). Restriction fragment length polymorphism of an amplified 439-bp segment of the 65-kDA heat shock protein has been shown to be a rapid and sensitive method for iden- tification of clinically significant ... See full document
5
Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S 23S rRNA Gene Internal Transcribed Spacer PCR Restriction Analysis
... amplicon-restriction analysis assay, designated ITSPRA, which could rapidly and efficiently identify and differentiate the individual MCC ...on identification of the 19 isolates were obtained ... See full document
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Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... 26 clinical MAC isolates, two novel allelic M. avium PCR-restriction enzyme analysis (PRA) variants were identified, differing from the ...sequencing. Identification of ... See full document
6
Detection and identification of mycobacteria in formalin fixed, paraffin embedded tissues by nested PCR and restriction enzyme analysis
... the identification of ...of PCR product used did not affect the digestion ...the analysis of the digests in agarose gels ...same restriction pattern. The restriction patterns of ... See full document
5
Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR Restriction Enzyme Analysis of hsp65
... Human isolates of Mycobacterium collected in 16 different states of Brazil were submitted to PCR-restriction analysis (PRA) of a 439-bp fragment of the hsp65 gene with HaeIII and ...in ... See full document
6
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... one pattern each for ...additional pattern for a single presump- tive ...39 isolates of this group (35 clinical isolates and 4 type strains) were studied, and in addition to the ... See full document
5
PCR and Restriction Endonuclease Analysis for Rapid Identification of Human Adenovirus Subgenera
... two isolates were identified as subgroup C, but one had been typed as Ad31 (subgroup A) and the other had been typed as Ad14 (subgroup B) by ...RE analysis of the whole ge- nome as Ad34 or Ad35 (subgroup ... See full document
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Use of PCR Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene Based Identification of Nocardia Species
... the clinical isolates indicated that (i) species discrimination was better with hsp65 than with 16S rRNA (larger distances between the closest species pairs) and (ii) combined analysis of the two ... See full document
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species ...with restriction enzymes such as MspI ... See full document
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Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... RFLP analysis. Good results were obtained when PCR-amplified DNAs from all ...aureus isolates from cows and sheep, a good relationship between antibiotic susceptibility type and RFLP pattern ... See full document
5
Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR restriction enzyme analysis, 16S rRNA gene sequencing, and DT1 DT6 PCR
... detailed analysis of all DT1-positive isolates (14 discrep- ant strains plus 5 ...12 clinical isolates of ...19 isolates (32%) gave PRA results compatible with the previ- ously ... See full document
6
An Outbreak of Keratitis Caused by Mycobacterium immunogenum
... species identification when analyzing ITS partial sequences, the high- est match ...outbreak isolates are concordant, and we propose that they are a variant of ...Outbreak isolates had ... See full document
7
Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques
... inaccurate identification of our ...by rapid and easy methods, such as analysis of the hsp65 gene, PCR detection of mig, or detection of DT1 or DT6 MAC-specific ...accurate ... See full document
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... Thermo-Start PCR master mix (Thermo Scientific) and ...the clinical isolates and controls was added in 2- l volumes in total reaction mixtures of 20 ... See full document
5
Molecular Analysis of Mycobacterium kansasii Isolates from the United States
... kansasii isolates from the United States by PCR restriction enzyme analysis (PRA) of the 441-bp Telenti fragment of the hsp-65 gene and pulsed-field gel electrophoresis (PFGE) of ... See full document
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Clostridium difficile Genotyping Based on slpA Variable Region in S Layer Gene Sequence: an Alternative to Serotyping
... sequence-based analysis has been used more successfully than serological anal- ysis for strain subtyping ...by PCR can be used for identification of ...blotting pattern when hybridized with ... See full document
7
Journal of Applied Pharmaceutical Science
... of actinomycete isolates from soils in Thailand, the isolation and screening of antimicrobial activity and identification of strains were determined based on the phenotypic and chemotaxonomic ... See full document
6
Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... Bacterial strains. The bacterial strains used in this study are described in Table 1. The 47 reference Staphylococcus species and subspecies were selected from the Polish Collection of Microorganisms, the Czech ... See full document
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Usefulness of the ID32 staph system and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates
... Usefulness of the ID32 Staph System and a Method Based on rRNA Gene Restriction Site Polymorphism Analysis for Species and Subspecies Identification of Staphylococcal Clinical Isolates 0[r] ... See full document
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