[PDF] Top 20 Cloning and Functional Analysis of Kaposi’s Sarcoma-Associated Herpesvirus DNA Polymerase and Its Processivity Factor
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Cloning and Functional Analysis of Kaposi’s Sarcoma-Associated Herpesvirus DNA Polymerase and Its Processivity Factor
... of herpesvirus processivity factors remain largely ...or functional motifs, except for the presence of a number of potential phosphorylation sites (data not ...virus processivity factors, PF-8 ... See full document
5
Negative Elongation Factor-Mediated Suppression of RNA Polymerase II Elongation of Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene Expression
... PFGE DNA marker and the 1-kb DNA marker, ...genomic DNA copy number. Genomic DNA was prepared from 293TBAC16 (wt) and 293TBAC16RTAstop (clone 4, RTAstop) either mock treated (0 hpi) or treated ... See full document
12
Kaposi's sarcoma-associated herpesvirus encodes a functional cyclin.
... Shotgun cloning and DNA sequencing. The complete DNA sequence of the 17-kb insert of phage lambda clone SY3-2 was determined by a shotgun ap- ...insert DNA of SY3-2 was sonicated (maximum ... See full document
8
Nuclear Transport of Epstein-Barr Virus DNA Polymerase Is Dependent on the BMRF1 Polymerase Processivity Factor and Molecular Chaperone Hsp90
... that DNA polymerase processivity factors of many human herpesviruses such as UL42 of herpes simplex virus 1 (HSV-1) (32), BMRF1 of EBV (33), UL44 of human cytomega- lovirus (HCMV) (34), PF-8 of ... See full document
10
Kaposi’s Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor
... A DNA fragment corresponding to the KSHV K9 was synthesized from BCBL-1 genomic DNA by PCR using primers containing a BamHI site at the 59 end and an EcoRI site at the 39 end for subsequent ...PCR-amplified ... See full document
8
Identification of the DNA Sequence Interacting with Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 1
... Kaposi’s sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8, a recently discovered human DNA tumor virus, is strongly associated with the development of Kaposi’s sar- ... See full document
5
Characterization of Human Herpesvirus 8 ORF59 Protein (PF-8) and Mapping of the Processivity and Viral DNA Polymerase-Interacting Domains
... Human herpesvirus 8 (HHV-8) or Kaposi’s sarcoma-associated herpesvirus (KSHV) ORF59 protein (PF-8) is a processivity factor for HHV-8 DNA polymerase (Pol-8) and is ... See full document
10
Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus
... Kaposi’s sarcoma-associated herpesvirus (KSHV, HHV-8) is a gammaher- pesvirus associated with several human ...malignancies. DNA methylation at CpG di- nucleotides is an epigenetic mark ... See full document
19
Kaposi's Sarcoma-Associated Herpesvirus ORF57 Is Not a Bona Fide Export Factor
... blot analysis confirmed that N-ORF-57-HA did not affect the unspliced cat gene en- coding transcript ...increased its cy- toplasmic level, as ...microscopy analysis and found that the N-ORF57-HA ... See full document
6
The Crystal Structure of PF-8, the DNA Polymerase Accessory Subunit from Kaposi's Sarcoma-Associated Herpesvirus
... the herpesvirus pro- cessivity factors and the sliding clamps suggest that they share a common ...of its mode of binding to DNA) the simplest of proteins, might be most similar to the ... See full document
14
A Mobile Functional Region of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein Independently Regulates DNA Binding and Protein Abundance
... a DNA fragment encoding ORF50 (aa 1 to 590) with or without the KK/EE mutation or a DNA fragment encoding ORF50 (aa 333 to 691) was PCR amplified and inserted into pET-22b (Novagen) at the NdeI and XhoI ... See full document
17
Functional p53 Signaling in Kaposi's Sarcoma-Associated Herpesvirus Lymphomas: Implications for Therapy
... curve analysis (data not ...fully functional in PEL, engaging multiple pathways to mediate the therapeutic effects of Nutlin-3 and DNA-damag- ing ... See full document
11
Genome-Wide Mapping of the Binding Sites and Structural Analysis of Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 2 Reveal that It Is a DNA-Binding Transcription Factor
... gene factor 3 (ISGF3) complexes, STAT1, and IRF-9, which results in the inhi- bition of ISG expression ...the DNA-binding domain, which is important in binding the promoters of IFNs and IFN- inducible genes ... See full document
11
Identification and Analysis of the K5 Gene of Kaposi's Sarcoma-Associated Herpesvirus
... Kaposi’s sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV-8), belongs to the gam- maherpesvirus subfamily and encodes ⬃ 80 open reading frames ...respective DNA and ... See full document
9
Phosphorylation of Kaposi's Sarcoma-Associated Herpesvirus Processivity Factor ORF59 by a Viral Kinase Modulates Its Ability To Associate with RTA and oriLyt
... sperm DNA-protein A-pro- tein G-Sepharose slurry for 1 h at 4°C with constant ...sperm DNA-protein A-protein G ...Eluted DNA was precipitated, treated with proteinase K at 45°C for 2 h, and ... See full document
15
Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Contains Two Functional Lytic Origins of DNA Replication
... Transfection of pDA12 along with pEXP50 into BCBL-1 cells resulted in a DpnI-resistant band, indicating that the pDA12 plasmid was replicated (Fig. 2A, left side, lane 1). When pEXP50 was omitted from the cotransfection ... See full document
7
Kaposi sarcoma–associated herpesvirus: immunobiology, oncogenesis, and therapy
... KSHV successfully subverts the cellular innate immune response to establish a lifelong latent reservoir in the infected host, primar- ily in B cells. The virus has evolved a number of mechanisms to ensure that virally ... See full document
12
Role of the Specific Interaction of UL112-113 p84 with UL44 DNA Polymerase Processivity Factor in Promoting DNA Replication of Human Cytomegalovirus
... retains DNA-binding and polymerase-binding activities and processivity activity but lacks the carboxy-terminal region re- quired for p84 ...and its truncation mutants that were ... See full document
13
HLA polymorphisms and detection of kaposi sarcoma-associated herpesvirus DNA in saliva and peripheral blood among children and their mothers in the uganda sickle cell anemia KSHV Study
... statistical analysis of familial clustering of KSHV infec- tion, among Noire-Marrons families in French Guiana ...KSHV DNA shedding in saliva among subjects carrying human leukocyte antigens (HLA) ... See full document
5
Characterization of the K1 protein of Kaposi sarcoma-associated herpesvirus and development of a novel vaccine for protection against rhadinovirus infection
... RRVΔLANA/GFP genome by Solexa sequencing reads. Solexa generates 35bp DNA reads. These were aligned to the RRV H26-95 genome using the reference sequence. The arrows on top represent individual RRV ORFs and the ... See full document
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