[PDF] Top 20 Conservation of Type Specific B Cell Epitopes of Glycoprotein G in Clinical Herpes Simplex Virus Type 2 Isolates
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Conservation of Type Specific B Cell Epitopes of Glycoprotein G in Clinical Herpes Simplex Virus Type 2 Isolates
... Glycoprotein G (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted amino-terminal portion and to a cell-associated, heavily ... See full document
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Specificity and affinity of binding of herpes simplex virus type 2 glycoprotein B to glycosaminoglycans.
... HSV-2 glycoprotein binding. Measurements of glycoprotein binding were performed on a BIAcore biosensor system with CM5 biosensor chips (Pharmacia Biosensor) as previously described (15, ...flow ... See full document
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Herpes Simplex Virus Type 2 Glycoprotein G-Negative Clinical Isolates Are Generated by Single Frameshift Mutations
... detect type-specific antibodies against mature gG-2 and gG-1 was performed with sera from patients from whom the gG-2-negative HSV-2 strains had been ...HSV-2 isolates ... See full document
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Dichotomy of Glycoprotein G Gene in Herpes Simplex Virus Type 1 Isolates
... Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody ...HSV-1 isolates. ... See full document
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Use of a Fragment of Glycoprotein G 2 Produced in the Baculovirus Expression System for Detecting Herpes Simplex Virus Type 2 Specific Antibodies
... Western blot (immunoblot) analysis. The gG-1 and gG-2 polypeptides, which were used as antigens in the ELISAs, were analyzed by SDS-PAGE. The polypeptides were preheated in SDS sample buffer for 4 min and ... See full document
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Truncation of Herpes Simplex Virus Type 2 Glycoprotein B Increases Its Cell Surface Expression and Activity in Cell-Cell Fusion, but These Properties Are Unrelated
... by cell-cell fusion is characteristic of herpes simplex virus (HSV) lesions, but the great majority of viruses isolated from such lesions produce only limited cell fusion in ... See full document
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Amplification of Reiterated Sequences of Herpes Simplex Virus Type 1 (HSV 1) Genome To Discriminate between Clinical HSV 1 Isolates
... HSV-1 isolates obtained from keratitis ...37 clinical corneal HSV-1 isolates are summarized in Table ...unrelated clinical isolates from 10 patients are shown in ...37 isolates ... See full document
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Divergence and Recombination of Clinical Herpes Simplex Virus Type 2 Isolates
... on specific, functionally important amino ...at specific sites or epitopes, the conflicting signals described here for the HSV-2 alignment were present in all three genes in- vestigated, US4, ... See full document
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Secreted Portion of Glycoprotein G of Herpes Simplex Virus Type 2 Is a Novel Antigen for Type Discriminating Serology
... a type-specific assay is the period of time from the time of transmission of HSV-2 to the time when antibodies are initially detected in the ...linear epitopes of the protein to a high degree ... See full document
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Variability of the Glycoprotein G Gene in Clinical Isolates of Herpes Simplex Virus Type 1
... to isolates 2, 5, 7, and ...HSV-1 isolates from patients 1 and 2 were identical to the consensus sequence, while isolates 7 and 9 carried several mutations within the immunodominant ... See full document
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Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B
... The highest frequency of IFN- ␥ -producing CD4 ⫹ T-cell re- sponses was detected against G4 (47.5%), while 42% of indi- viduals responded to G14 and G9. In comparison, only 5 to 38% of individuals showed positive ... See full document
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Locations of herpes simplex virus type 2 glycoprotein B epitopes recognized by human serum immunoglobulin G antibodies.
... (or epitopes) that reacts with HSV-2 anti- bodies in a virus type-specific ...a virus type-specific manner but gave false-positive results in these three HSV-1 1 ... See full document
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Heptad Repeat 2 in Herpes Simplex Virus 1 gH Interacts with Heptad Repeat 1 and Is Critical for Virus Entry and Fusion
... For cell coexposure, the cells were exposed to increasing concentrations of the peptides and the viral inoculum for 90 min at ...For cell preexposure, cells were incubated with a single concentration of ... See full document
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Postentry Events Are Responsible for Restriction of Productive Varicella-Zoster Virus Infection in Chinese Hamster Ovary Cells
... of cell viability as well as an indicator of nonspecific effects on  -Gal production caused by lysosomo- tropic ...on virus entry, CHO-IE  8 cells were preincubated with medium containing 50 mM ammonium ... See full document
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Pseudorabies Virus Glycoprotein M Inhibits Membrane Fusion
... rabies virus (PrV) ...of cell fusion was quantitated 27 to 42 h after ...glycoproteins B (gB), gD, gH, and gL resulted in formation of polykaryocytes, as has been shown for homologous proteins of ... See full document
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Infection of Chinese Hamster Ovary Cells by Pseudorabies Virus
... identify virus receptors, a successful approach has in- volved expression cloning in cells which are resistant to infec- tion by the respective virus due to absence of the ...a specific defect in ... See full document
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The Genome Sequence of Herpes Simplex Virus Type 2
... traversed by LATs and in the LAT orientation. It is antisense to and largely overlaps the ORF of gene RL1 (43). Mutation of an ICP4 binding motif in the proposed promoter for ORF P allowed expression of the ORF in ... See full document
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Fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein D.
... A region common to herpes simplex virus type 1 and 2 gD amino acid residues 11 to 19 and two sites specific for herpes simplex virus type 2 gD one determined by proline at position 7, th[r] ... See full document
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Identification and characterization of a novel structural glycoprotein in pseudorabies virus, gL.
... Identification and expression of human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 g[r] ... See full document
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Functional Relevance of the Transmembrane Domain and Cytoplasmic Tail of the Pseudorabies Virus Glycoprotein H for Membrane Fusion
... Function of the PrV gH cytoplasmic domain. To investigate the functional rele- vance of the 19-amino-acid (aa) cytoplasmic domain (CD) of PrV gH, different truncated proteins were generated lacking either the C-terminal ... See full document
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