[PDF] Top 20 Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
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Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... the human growth hormone, this mutagenesis approach resulted in the identifi- cation of residues involved in hormone binding ...only 1 of 33 HIV-1 RT mutants was ts, and a previous report describing ... See full document
8
Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility
... tree construction we also included the RT sequences of six subtype B HIV-1 isolates (LAI, SF2, MN, OYI, JRFL, and JRCSF), three subtype D sequences (ELI, NDK, and Z2Z6), two subtype A sequences (UG037 and ... See full document
5
Human Immunodeficiency Virus Mutagenesis during Antiviral Therapy: Impact of Drug-Resistant Reverse Transcriptase and Nucleoside and Nonnucleoside Reverse Transcriptase Inhibitors on Human Immunodeficiency Virus Type 1 Mutation Frequencies
... K103N mutant can emerge in patients treated with efavirenz-containing therapy (1), and the Y318F mutant was associated with a decrease in susceptibility to all NNRTIs ...the virus mutation ... See full document
13
Involvement of Novel Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutations in the Regulation of Resistance to Nucleoside Inhibitors
... leukemia virus RT, and amplified with Amplitaq-Gold polymerase enzyme by using two different sequence-specific primers for 40 ...the reverse transcriptase open reading frame) were full-length ... See full document
13
Resistance pattern of human immunodeficiency virus type 1 reverse transcriptase to quinoxaline S-2720.
... Indeed, the quinoxaline derivative has broad-spectrum activity against various mutant HIV-1 strains that are resistant to other HIV-1-specific RT inhibitors; i.e., quinoxaline S-2720 is [r] ... See full document
7
Attenuated replication of human immunodeficiency virus type 1 with a didanosine-selected reverse transcriptase mutation.
... in human immunodeficiency virus type 1 reverse transcriptase (RT) develops as a consequence of didanosine (ddI) therapy and is associated with a decreased susceptibility ... See full document
7
Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... In summary, substitutions at 9 of the 11 RT motif B positions subjected to random mutagenesis altered viral susceptibility to one or more nucleoside analogs, and several mutations con- ferred altered sensitivity to the ... See full document
10
Effects of Amino Acid Substitutions at Position 115 on the Fidelity of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... been reported in the literature (reviewed in reference 23). However, when the mutations are examined directly, it is clear that our data differ substantially from what has been published. Although we found hot spots in ... See full document
7
Individual Contributions of Mutant Protease and Reverse Transcriptase to Viral Infectivity, Replication, and Protein Maturation of Antiretroviral Drug-Resistant Human Immunodeficiency Virus Type 1
... Genome stability of resistant isolates. The stability of viral Gag, PR, and RT sequences in the absence of drug pressure was assessed in PBMCs for up to 100 viral life cycles (20). Limited genetic evolution in terms of ... See full document
10
Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
... closed 1-LTR and 2-LTR circular forms are believed to be the products of recombination and ligation of LTRs flanking the linear viral DNA, respectively (9, ...Both 1-LTR and 2-LTR circle junctions could be ... See full document
6
Characterization and Structural Analysis of Novel Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Involved in the Regulation of Resistance to Nonnucleoside Inhibitors
... leukemia virus RT, and amplified with AmpliTaq Gold polymerase enzyme by using two different sequence-specific primers for 40 ...and mutant residues at a single position were considered to have the ... See full document
13
A Mutation in the 3′ Region of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase (Y318F) Associated with Nonnucleoside Reverse Transcriptase Inhibitor Resistance
... The RT mutation P236L has been linked to high-level in vitro resistance to DLV but not other NNRTIs (4). In response to DLV monotherapy, however, the K103N and Y181C muta- tions developed rapidly while the P236L mutation ... See full document
5
Cross-Linking of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase to Template-Primer
... each mutant were modified with one of the two thiol-reactive heterobifunctional photocross- linking reagents—APTP (azide) or BATDHP ...(Fig. 1). The polymerase and RNase H activities of the mutant ... See full document
11
In Vitro Characterization of a Simian Immunodeficiency Virus-Human Immunodeficiency Virus (HIV) Chimera Expressing HIV Type 1 Reverse Transcriptase To Study Antiviral Resistance in Pigtail Macaques
... Prolonged use of RT inhibitors may lead to the appearance of resistant virus. Common mutations leading to NNRTI resis- tance include K103N, Y181C, and Y188C (40), which contribute to the failure of ... See full document
9
Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... and mutant amino acids at RT codons 41, 184, and 215 showed that two laboratories that used sequencing by hybridization performed at least as well as 21 other laborato- ries that used cycle sequencing ... See full document
7
Characterization of Intracellular Reverse Transcription Complexes of Human Immunodeficiency Virus Type 1
... very sensitive to nuclease digestion. Strong-stop DNA was very sensitive to nuclease digestion in the RTC prepared 1 h after acute ...MoMLV, 1-h fractions containing the peak of MoMLV or HIV ... See full document
10
Mutations in the Thumb Allow Human Immunodeficiency Virus Type 1 Reverse Transcriptase To Be Cleaved by Protease in Virions
... Although human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) has been extensively studied, there are still significant questions about the ... See full document
9
Characterization of human immunodeficiency virus type 1 reverse transcriptase by using monoclonal antibodies: role of the C terminus in antibody reactivity and enzyme function.
... Mapping of this epitope with C-terminally truncated mutants revealed that this MAb mapped near the C terminus and thus confirmed that the 51-kDa protein is derived from the 66-kDa protei[r] ... See full document
6
Sensitive Phenotypic Detection of Minor Drug Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Variants
... and reverse transcription ...select reverse transcription events. Elements carrying wild-type RT (H2BX) and the NNRTI resistance allele K103N were placed on each plate as ... See full document
9
Mutations That Abrogate Human Immunodeficiency Virus Type 1 Reverse Transcriptase Dimerization Affect Maturation of the Reverse Transcriptase Heterodimer
... abrogate human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) dimerization on virus replication is not known, as mutations shown previously ... See full document
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