[PDF] Top 20 Context-Dependent Phenotype of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation

... Retroviral nucleocapsid (NC) carries out important func- tions throughout the entire retroviral life cycle (2, ...the virus-encoded protease, NC coats viral genomic RNA and subsequently influences early ... See full document

... of human immunodeficiency virus (HIV) depends on the processing of Gag and Pol polyproteins by the viral protease, making this enzyme a prime target for anti-HIV ...the nucleocapsid-p1 (NC-p1) ... See full document

... the nucleocapsid (NC) domain of the Gag polyprotein disrupts assembly, presumably because NC is required for ...polymerization. Human immunodeficiency virus type 1 NC possesses ... See full document

... viral context indicate that deletions and amino acid substitutions in the NC coding sequence result in the production of poorly infectious or noninfectious viruses (4, 21, 26–28, ...kemia virus NCp10 was ... See full document

... We proposed several possible mechanistic models for the unique T20-dependent replication phenotype (3). First, it could be envisaged that the T20 peptide is actively involved in the formation of the 6-helix ... See full document

... A92E virus infection in HeLa cells (38, ...the context of TRIMCyp or artificial CypA oligomers, increases capsid-binding avidity and leads to accelerated HIV-1 capsid disassembly and inhibition of ... See full document

... from virus-expressing HeLa ...profound virus release defects in HeLa ...p6 mutation in biological and biochemical analyses and by electron microscopy, we defined the role of p6 in particle release ... See full document

... To further characterize the inhibition and loss of viral infectivity induced by the A1752, we examined the syn- thesis of RT products in the virion-infected cells. We measured the minus strand strong-stop (-)ssDNA, an ... See full document

... mutant gp41 cross-linking pattern, we observed that gp41 N termini are cross-linked. Lysine 678, which is close to the transmembrane sequence, was readily cross-linked to Lys-678 on other monomers within the oligomeric ... See full document

... Deletion of the V2 loop from the SF128A envelope did not alter the overall conformation of that protein, as it retained its ability to bind to CD4 (Fig. 4B). However, neither the SF128A ⌬ V2 and SF170 ⌬ V2 envelopes were ... See full document

... The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA ...deletion human ... See full document

... the mutation on the molecule, may exert a dominant negative effect on the function carried out by wild-type ...transdominant phenotype would be distinct functional ... See full document

... the nucleocapsid (NC) domain is the most conspicuous RNA-binding region of the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein, we have previously shown that ... See full document

... extracellular virus particles. If the average diameter of HIV-1 particles is around 100 nm (70) and assuming that the particle is a sphere, the volume is ⬃ 5 ⫻ 10 ⫺19 ...cell type but are typically 3 ... See full document

... 4). A similar effect was observed when a deletion that removed the first CCHC motif and a few flanking residues ( ⌬ 15–34) was combined with a point mutation that changed the second CCHC motif to SCHC (lane 5). ... See full document

... of 1 to 2% integration at the highest IN concentration used, while in the presence of an optimal concentration of NCp7, we obtained up to 40% integration by using 150 nM ... See full document

... NC plays roles in nearly all steps of the viral life cycle. As a domain within the Gag polyprotein, NC specifically binds and incorporates viral genomic RNA into virions (reviewed in ref- erences 5 and 63) and drives ... See full document

... The two NL4-3 mutants that had low abilities to establish chronic virus producers NLAU and NLAUAN exhibited high levels of cell death during the acute phase of infection.. By contrast, t[r] ... See full document

... are human osteosarcoma cells (HOS) that express CD4 and one of several HIV ...by phenotype and coreceptor usage, there were neu- tralization-sensitive and neutralization-resistant ...the virus ... See full document

... this phenotype remains unknown, it does not appear to involve the role of the p1 coding sequence in ribo- somal frameshifting, because the slippery sequence at which frameshifting occurs (30) was not required to ... See full document