[PDF] Top 20 Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase.
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Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase.
... 5721 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Previous studies showed that an isolated human immunodeficiency virus type 1 HIV-1 RNase H domain expressed as a fus[r] ... See full document
9
Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family.
... each virus per cell) and harvested 72 h ...KCl, 1 mM EDTA, 1 mM dithiothreitol, protease inhibitor cocktail), homogenized with a Dounce homogenizer (30 strokes, 4 8 C), and centrifuged for 30 ... See full document
7
Mutants of Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcriptase Resistant to Nonnucleoside Reverse Transcriptase Inhibitors Demonstrate Altered Rates of RNase H Cleavage That Correlate with HIV-1 Replication Fitness in Cell Culture
... in RNase H activity contribute to the modest reduction in replication fit- ness of Y181C relative to wild-type virus, in part because this mutant also demonstrates a reduction in ... See full document
12
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... the human growth hormone, this mutagenesis approach resulted in the identifi- cation of residues involved in hormone binding ...RNA polymerase resulted in conditional mu- tants defective in RNA synthesis ... See full document
8
RNase H Requirements for the Second Strand Transfer Reaction of Human Immunodeficiency Virus Type 1 Reverse Transcription
... 50-mer DNA oligo- nucleotide which was complementary with the 18 nucleotides of the RNA ...[Exonuclease(⫺)] polymerase. The ex- tended 50-mer RNA-DNA oligonucleotide was then annealed to the 26-mer ... See full document
9
Mutations That Abrogate Human Immunodeficiency Virus Type 1 Reverse Transcriptase Dimerization Affect Maturation of the Reverse Transcriptase Heterodimer
... wild-type virus for the HX-W401L and HX-W401A ...the virus, allowing cleavage of the RNase H domain from one of the p66 monomers to ...RT activity observed for the ... See full document
11
Virion Instability of Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) Mutated in the Protease Cleavage Site between RT p51 and the RT RNase H Domain
... in virus spread assays in MT-2 cells ...proviral DNA produced by these “recovered” mutants indicated that in one group of mu- tants (E438N and F440A/Y441A) the mutated RT p51-RNH cleavage site had reverted ... See full document
10
Effects of Human Immunodeficiency Virus Type 1 Resistance to Protease Inhibitors on Reverse Transcriptase Processing, Activity, and Drug Sensitivity
... plasma virus were compared to iso- genic clones harboring the corresponding pretherapy protease ...each virus pair and analyzed by Western blotting with mono- clonal antibodies that recognize RT ...the ... See full document
5
Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity.
... affecting polymerase activity (2, 14). The X-ray crystal structures of HIV-1 RT heterodimers show that C280 lies near the middle of a helix I in the thumb subdomains of both p66 and ...the ... See full document
5
The Impact of Multidideoxynucleoside Resistance-Conferring Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase on Polymerase Fidelity and Error Specificity
... 15 h before scoring for mutant plaques that did not display the bright blue color of the wild-type ...in 1 ml of ...gapped-duplex DNA and scoring for mutants as described ...phage DNA. ... See full document
6
The M184V Mutation in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 Impairs Rescue of Chain-Terminated DNA Synthesis
... the polymerase-active site of RT and other polymerases ...“palm” subdomains of RT are required to confer resistance to AZT (22); however, enzymes that contain a combination of amino acid substitutions fre- ... See full document
7
Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Target Distinct Phases of Early Reverse Transcription
... its DNA polymerase ...nascent DNA chain by RT, result in the termination of DNA ...of virus isolated from patients taking NVP ...inhibitory activity against Taq DNA ... See full document
10
Selection of Mutations in the Connection and RNase H Domains of Human Immunodeficiency Virus Type 1 Reverse Transcriptase That Increase Resistance to 3′-Azido-3′-Dideoxythymidine
... Cells and viruses. MT-2 cells (AIDS Research and Reference Reagent Pro- gram, National Institute of Allergy and Infectious Diseases, National Institutes of Health) were cultured in RPMI 1640 (Whittaker MA Bioproducts, ... See full document
8
Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.
... Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli.. Reconstitution in vit[r] ... See full document
9
Comparison of Second-Strand Transfer Requirements and RNase H Cleavages Catalyzed by Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) and E478Q RT
... Figure 1 summarizes the second-strand transfer assay with substrates possessing truncations in the 5⬘ RNA ...Step 1 represents the input substrates for the ⌬6, ⌬9, and ⌬12 constructs, in which the ... See full document
12
HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells
... The prokaryotic expression vector, p6H-RT-PR, was kindly provided by Dr Giovanni Maga and has been described elsewhere [16]. GST-HuR was constructed by PCR ampli- fication of the HuR gene from the image clone # ... See full document
14
Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility
... the reverse transcriptase (RT) regions of the pol genes of 14 human immunodeficiency virus type 1 (HIV-1) isolates from Romanian patients, which were classified as ... See full document
5
Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... The polymerase domain of HIV-1 RT contains structural motifs that are conserved in all reverse transcriptases and RNA-dependent viral polymerases ...(Fig. 1) (4, 21, ...of DNA synthesis ... See full document
10
Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide.
... The three known enzymatic activities associated with the DNA polymerase of avian RNA tumor viruses were compared using polyC oligodG to measure RNA-directed DNA synthesis, polydC oligodG[r] ... See full document
6
Human Immunodeficiency Virus Mutagenesis during Antiviral Therapy: Impact of Drug-Resistant Reverse Transcriptase and Nucleoside and Nonnucleoside Reverse Transcriptase Inhibitors on Human Immunodeficiency Virus Type 1 Mutation Frequencies
... ⫺ 1, ⫺ 2, and ⫹ 1 frameshift mutations and dislocation mutagenesis of HIV-1 RT ...of DNA replication fidelity are generated during the catalytic cycle of the various polymerases, in which ... See full document
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