[PDF] Top 20 Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin.
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Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin.
... We have also used DNA footprinting techniques to show indirectly that the assembly of T antigen leads to stepwise and cooperative binding to the early and late halves of the SV40 core or[r] ... See full document
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Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA Unwinding
... of simian virus 40 large tumor (T) antigen on threonine 124 is essential for viral DNA rep- ...mutant T antigen (T124A), in which this threonine was replaced by alanine, ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... large T antigen, which contains 708 amino acids, plays vital roles in coordinating viral DNA repli- cation and transformation (6, 18, 47, ...and functional domains of T antigen include ... See full document
11
Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
... Because T antigen covers only the center palindrome of the origin at 37°C, the binding of T antigen to region I or of the positive regulatory protein Spl to the 21-bp repeats 13 could al[r] ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... the T-ag obd has also been ...of T-ag to form double hexamers ...arranged T-ag obd’s. (A) Surface representation of the T-ag obd ...arranged T-ag obd’s is presented below each ... See full document
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Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... papovavirus simian virus 40 (SV40) minichromosome has been used extensively as a model to study the mechanism of eukaryotic DNA replication (see references 8 and 24 and references ... See full document
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Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... In a previous publication (77), we proposed that, based on results obtained with the bacteriophage T7 gene 4 helicase, LTag undergoes an open-filament-to-closed-ring transition when assembly of the hexamer with ... See full document
10
Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... the assembly process was confirmed by the results obtained with the 48-bp site IIm ⫹ EP oligonucle- otide ...3); T-ag assembly on this molecule was limited to hexamer formation ...pendent ... See full document
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The Simian Virus 40 Core Origin Contains Two Separate Sequence Modules That Support T-Antigen Double-Hexamer Assembly
... core origin support the assembly of T-ag double hexamers (this study and references 32, 33, and 35), the entire core origin region is needed for initiation of DNA unwinding (13, ...core ... See full document
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The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting.
... The failure of the zinc finger mutant T antigens to form hexamers in response to ATP may account for defects in subsequent steps in the initiation of DNA replication; we propose that hex[r] ... See full document
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... DNA replication in eukaryotes have been productively studied by using simian virus 40 (SV40) (reviewed in references 17 and ...DNA replication requires a single viral factor, the large ... See full document
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A functional simian virus 40 origin of replication is required for the generation of a super T antigen with a molecular weight of 100,000 in transformed mouse cells.
... 2 A Functional Simian Virus 40 Origin of Replication Is Required for the Generation of a Super T Antigen with a Molecular Weight of 100,000 in Transformed Mouse Cells Received 28 March 1[r] ... See full document
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Two separable functional domains of simian virus 40 large T antigen: carboxyl-terminal region of simian virus 40 large T antigen is required for efficient capsid protein synthesis.
... COLE',2* Department Genetics, Yale University School of Medicine, New Haven, Connecticut 065101; and Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 037562* [r] ... See full document
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Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... (Tag antigen monoclonal antibody [MAb] 419 and E1A MAb M73 [a gift of Ed Harlow, Massachusetts General Hospital Cancer Center, Boston, ...for 40 min at ... See full document
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Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
5
cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Binding of[r] ... See full document
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Mutual functional antagonism of the simian virus 40 T antigen and the hepatitis B virus trans activator.
... Positive and negative regulation of transcription in vitro: enhancer binding protein AP-2 is inhibited by SV40 T antigen.. Production of simian virus 40 large tumor antigen in bacteria: [r] ... See full document
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Functional implications of oligomerization of simian virus 40 large T antigen during lytic virus infection.
... To determine the distribution of various forms of T antigen on the sucrose density gradients at a quantitative level, the corresponding gel bands were excised, eluted with 0.2 ml of 60% [r] ... See full document
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