[PDF] Top 20 A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription.
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A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription.
... than reverse transcriptase are involved in the control of HIV-1 reverse ...with reverse transcriptase (45, 46). Since the initiation of reverse transcription likely occurs within ... See full document
11
Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
... postcoculture, virus from pHXB2(His-AC-TGT) with M184V RT contained an increased level of p24 antigen in the supernatant of the culture, although the level was not comparable to that of the wild-type or ... See full document
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Genetic Dissociation of the Encapsidation and Reverse Transcription Functions in the 5′ R Region of Human Immunodeficiency Virus Type 1
... the TAR element (32), as well as the secondary structure of the poly(A) hairpin (12), are required for fully efficient HIV-1 genomic RNA ...the TAR hairpin, from nucleotides 14 to 112, ... See full document
9
The Human Immunodeficiency Virus Type 1 TAR RNA Upper Stem-Loop Plays Distinct Roles in Reverse Transcription and RNA Packaging
... the role of TAR in reverse transcription is dependent on its secondary ...which TAR exerts its effect remains unknown, but it may in- volve the binding of viral or cellular proteins ... See full document
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Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Target Distinct Phases of Early Reverse Transcription
... the TAR RNA element (6, ...that reverse transcription elongation appears to be induced under conditions that favor the completion of pro- viral DNA ...intact virus. Induction of ... See full document
10
An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA
... FIG. 4. Lack of RNA packaging from a plasmid that expresses RNA which remains in the nucleus. (A) Schematic representation of the plasmids used in this study. pHR1256 contains a small in-frame deletion in pol as well as ... See full document
9
Human immunodeficiency virus type 1 TAR element revertant viruses define RNA structures required for efficient viral gene expression and replication.
... The TAR element is a viral regulatory element extending from 1 1 to 1 60 in the human immunodeficiency virus type 1 (HIV-1) long ... See full document
8
Human Immunodeficiency Virus Type 1 Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex
... The human immunodeficiency virus type 1 (HIV-1) integrase protein (IN) is essential for integration of the viral DNA into host cell ...affect virus replication at ... See full document
10
The TAR Hairpin of Human Immunodeficiency Virus Type 1 Can Be Deleted When Not Required for Tat-Mediated Activation of Transcription
... The human immunodeficiency virus type 1 (HIV-1) RNA genome contains a terminal repeat (R) region that encodes the transacting responsive (TAR) hairpin, which is essential ... See full document
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Vpx Is Critical for Reverse Transcription of the Human Immunodeficiency Virus Type 2 Genome in Macrophages
... the human immunodeficiency virus type 2 (HIV-2) group carry a vpx gene that encodes virion-associated Vpx ...for virus replication in estab- lished cell lines and primary lymphocyte ... See full document
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Sequences in the 5′ and 3′ R Elements of Human Immunodeficiency Virus Type 1 Critical for Efficient Reverse Transcription
... of reverse transcrip- tion ...intravirion reverse transcription was determined using an endogenous reverse transcriptase assay in which nu- cleotides and small amounts of detergent are ... See full document
11
Contribution of the C terminal tri lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import
... for reverse transcription, integration or undefined post-nuclear entry steps ...component, human lens epithelium- derived growth factor/transcription coactivator p75 (LEDGF/p75) and LEDGF/p75 ... See full document
15
Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription
... in reverse transcription was also seen in endogenous reverse transcription ...of reverse transcription, al- though its exact role in this process has not been ...of ... See full document
10
Association of Human Immunodeficiency Virus Type 1 Vif with RNA and Its Role in Reverse Transcription
... PCR of intravirion nucleic acids. PCR was performed according to the stan- dard manufacturer’s conditions (Perkin-Elmer). PCRs were run at 94°C for 30 s, 55°C for 30 s, and 72°C for 45 s for 30 cycles, followed by a ... See full document
8
Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... plasma human immunodeficiency virus type 1 (HIV-1) RNA levels has become an important tool for identifying individuals likely to benefit from antiretroviral ther- apy (12, 15, ... See full document
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Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription
... and reverse primer 5⬘-CTG CTA GAG ATT TTC CAC ACT GAC, which amplify region 496 to 635 of NL4-3 (GenBank accession ...and reverse primer 5⬘-CTG AAG TTC TCA GGA TCC ...the human genomic DNA from the ... See full document
7
Incorporation of Human Immunodeficiency Virus Type 1 Reverse Transcriptase into Virus-Like Particles
... The membrane-binding capacity of the RT-IN derivatives is not completely compatible with their particle incorporation efficiency. Since all of the RT-IN derivatives retained N-termi- nal 14 MA amino acid residues, each ... See full document
11
Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription.
... Su 1 packaged in virions to initiate re- verse transcription. Instead of using heat-annealed tRNA as the source of the primer for RT, we used total viral RNA extracted from virions as the source of ... See full document
7
A novel cis-acting element that controls transcription of human immunodeficiency virus type 1 DNA, depending on cell type.
... 6334-6338 0022-538X/91/116334-05$02.00/0 Copyright C 1991, American Society for Microbiology A Novel cis-Acting Element That Controls Transcription of Human Immunodeficiency Virus Type 1[r] ... See full document
5
Defective Replication in Human Immunodeficiency Virus Type 1 When Non-Primers Are Used for Reverse Transcription
... medium. Viral replication (CA) was monitored every 3 days, and cells were passaged as described for panels A and B. Viral accumulation over successive 3-day periods was plotted on a linear (C) or logarithmic (D) scale. ... See full document
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