[PDF] Top 20 Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... the T-ag obd has also been ...of T-ag to form double hexamers (60). In our crystal structure, loop B3 has high B factors and thus is prob- ably relatively ...2. Structure of spirally ... See full document
9
Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... We next addressed whether both pentanucleotide recogni- tion sites in the active pentanucleotide double mutants (Fig. 6B) were required for stable binding. To conduct these assays, oligonucleotides containing a ... See full document
14
Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... to binding. Satisfyingly, the structure of Meinke et ...high-affinity binding and that its interaction with specific and nonspecific DNA is dramatically increased at lower pH and reduced at higher ... See full document
13
Discrete regions of simian virus 40 large T antigen are required for nonspecific and viral origin-specific DNA binding.
... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document
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Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain.
... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document
9
Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... small t helper function in SV40 transforma- tion and metabolic stabilization of p53 in complex with large ...with large T actively contributes to phenotypic trans- formation by SV40, both ... See full document
9
Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... tumor virus studies ...small t antigen, respectively), or dlA2433, a mutant with a misfolded carboxyl half due to a deletion of aa 587 to 589 ...carboxyl-region T-antigen functions, ... See full document
8
Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids.
... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document
8
The zinc finger region of simian virus 40 large T antigen.
... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ... See full document
7
Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) ... See full document
11
Topoisomerase I Associates Specifically with Simian Virus 40 Large-T-Antigen Double Hexamer–Origin Complexes
... inhibit T antigen in a func- tional assay. We generated a labeled, origin-containing, circu- lar, 388-bp DNA ...with T antigen and various amounts of topo I to determine the conditions ... See full document
9
Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... detect binding of full-length T antigen to fluorescent probes under the same salt ...D, T antigen was able to bind to the TBS-containing probe in a sequence-specific manner but with a ... See full document
7
The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...replication, T antigen must interact with three ... See full document
7
The T-antigen-binding domain of the simian virus 40 core origin of replication.
... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document
7
Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
5
Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ... See full document
6
Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document
8
The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
6
Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the structure of the T-Ag OBD and its interactions with ...the structure of the T-Ag OBD by nuclear magnetic resonance (NMR) meth- ods (30) and established that it is a monomer in ...the ... See full document
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