[PDF] Top 20 Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA.
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Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA.
... Virus recovered by plaque assay on chicken cells is shown at the right, where + signifies that the mutant is viable and forms plaques indistinguishable from those produced by RNA transcr[r] ... See full document
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Modification of the 5′ Terminus of Sindbis Virus Genomic RNA Allows nsP4 RNA Polymerases with Nonaromatic Amino Acids at the N Terminus To Function in RNA Replication
... mutant RNA, and a number of suppres- sor mutations in such stocks have been mapped ...the 5⬘ termini of the genomic RNAs of 13 ...the sequence at the exact 5⬘ end, as illustrated in ... See full document
9
Mutations in the 5′ Nontranslated Region of Bovine Viral Diarrhea Virus Result in Altered Growth Characteristics
... the 5 ⴕ and 3 ⴕ sequences of BVDV ...genomic sequence was almost completely known (29). Only the 5⬘-terminal sequence of the CP7 genomic RNA comprising the 5⬘-proximal 21 to 23 ... See full document
11
A novel viral RNA species in Sindbis virus-infected cells.
... In Sindbis virus (SIN), the first two-thirds of the incoming genomic RNA is translated to yield two nonstructural polypro- teins (nsPs), nsP123 and ...the RNA-dependent RNA polymerase ... See full document
10
The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.
... PVX 5 9 NTR is likely to contain multiple cis-acting regulatory ...(53) defined the 5 9 NTR as two regions, the a sequence con- taining nt 1 to 41 (excluding the cap structure), which is AC ... See full document
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Rearrangements in the 5′ Nontranslated Region and Phylogenetic Analyses of Cucumber Mosaic Virus RNA 3 Indicate Radial Evolution of Three Subgroups
... the 5⬘ NTR or of the CP gene were initially aligned with the Pileup program of the Wisconsin Package, version ...the SEQUENCE editing and analysis program, version ...user- defined weights, and gaps ... See full document
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Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5' nontranslated RNA are impaired in RNA synthesis.
... 116-144 virus sequenced following pas- sage 3 (31 8 C), the sequence of the passage 6 D 116-144 rever- tant between nt 70 and 190 contained a single U-to-G trans- version at nt 114 ...original ... See full document
9
Attenuation of Sindbis virus neurovirulence by using defined mutations in nontranslated regions of the genome RNA.
... Mutant 5NTd15-25 grew well in cultured mouse cells and was as virulent as the parental virus in mice note that it grew poorly in chicken cells and moderately well in mosquito cells, howe[r] ... See full document
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Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
... the 5⬘ NTR are not required for IRES ...the 5⬘ end of a viral RNA genome are very important determinants for replication, we anticipated that the same might be true for ...nucleotides 5 to 20 ... See full document
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Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication
... A virus consists of eight negative- stranded RNA segments ...efficient virus replication, all eight viral RNA (vRNA) segments must be packaged into progeny ...and 5 ⬘ ends of the vRNA ... See full document
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Nonhomologous RNA-RNA recombination events at the 3' nontranslated region of the Sindbis virus genome: hot spots and utilization of nonviral sequences.
... crinkle virus and its satellite RNAs ...a sequence and/or structure associated with these two ...little sequence homology could be demonstrated between the two hot spot loci PD1 and PD2, similar ... See full document
12
3′ Nontranslated RNA Signals Required for Replication of Hepatitis C Virus RNA
... additional mutations within the VR in an effort to better characterize the requirements for sequence in this region and possibly to define the reasons for the significant loss of replication capacity in the ... See full document
12
Effects of Defined Mutations in the 5′ Nontranslated Region of Rubella Virus Genomic RNA on Virus Viability and Macromolecule Synthesis
... or RNA band was quantitated with a Fujix BAS1000 Bio Imaging Analyzer (Fuji Photo Film, Tokyo, Japan), using software provided by the ...suboptimal RNA secondary structures were determined by using the ... See full document
10
Mutations within the 5' nontranslated RNA of cell culture-adapted hepatitis A virus which enhance cap-independent translation in cultured African green monkey kidney cells.
... One virus, HM175/P16, which replicates very poorly in primates compared with HM175/wt, contains 19 mutations from the wild-type sequence which are scattered throughout the ...these mutations ... See full document
9
Mosquito homolog of the La autoantigen binds to Sindbis virus RNA.
... the 5 9 and 3 9 termini of the open reading frame were designed to create an NdeI restriction site at the 5 9 end that contained the initiation codon and a HindIII restriction site containing part of the ... See full document
9
Genesis of Sindbis virus by in vivo recombination of nonreplicative RNA precursors.
... released virus was recovered. Fresh BHK cells were infected with 5 to 10 PFU of recovered virus per cell and labeled with uridine, and 5 to 7 mg of RNA was separated on a ...of ... See full document
11
Template-Dependent Initiation of Sindbis Virus RNA Replication In Vitro
... HCV RNA transcripts ...initiate RNA synthesis, a transcript containing a predicted 39-terminal hairpin was generated from a deleted form of the yellow fever virus ge- nome ...reaction, RNA ... See full document
8
Identification and Characterization of Sindbis Virus RNA-Host Protein Interactions
... pathogens, RNA viruses must interact with the host cell machinery to establish and complete their life ...phaviral RNA-protein interactions has not been ...as RNA-binding proteins, has not been fully ... See full document
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Mutations in the Endodomain of Sindbis Virus Glycoprotein E2 Define Sequences Critical for Virus Assembly
... with RNA transcribed from either wild type or each of the E2 endodomain deletion mutants ( ⌬ 406-07, ⌬ 409-411, and ⌬ 414-417) as described ...with 5% uranyl acetate in distilled water for 60 min and in ... See full document
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Host Factors Associated with the Sindbis Virus RNA-Dependent RNA Polymerase: Role for G3BP1 and G3BP2 in Virus Replication
... viral RNA-dependent RNA poly- merase, at early (6 h) and late (12 h) times of infection with ...influence RNA replication and ...a virus expressing GFP containing a 3 ⫻ Flag epitope tag (TE/ ... See full document
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