[PDF] Top 20 Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
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Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
... 108 blood specimens from pediatric patients with suspected DENV infection presenting to the Queen Sirikit National Institute of Child Health (The Children’s Hospital, Bangkok, Thailand) from years 2003 and ...of ... See full document
6
Simultaneous detection and differentiation of dengue virus serotypes 1 4, Japanese encephalitis virus, and West Nile virus by a combined reverse transcription loop mediated isothermal amplification assay
... flexible reverse-transcription loop-mediated isothermal amplification (RT-LAMP) unit for the detection and differentiation of dengue virus serotypes 1-4 (DENV1-4), Japanese encephalitis ... See full document
9
Detection of Canine Distemper Virus Nucleoprotein RNA by Reverse Transcription PCR Using Serum, Whole Blood, and Cerebrospinal Fluid from Dogs with Distemper
... RT- PCR with all three different body fluids (serum, CSF, and whole blood), sensitivity was increased, showing a heteroge- neous distribution of CDV RNA in different body compart- ...for ... See full document
10
Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
... syncytial virus (hRSV) is one of the major causes of viral respiratory tract disease in young children and infants ...6). Virus isolation by cell culture and direct fluorescent-antibody assay (DFA) ... See full document
5
Detection of Colorado tick fever virus by using reverse transcriptase PCR and application of the technique in laboratory diagnosis
... early detection of CTF viral infection (12), is inherently subjective, and virus isolation (VI) from patient erythrocytes is time-consuming ...CTF virus assign a positive result to patient ... See full document
6
Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... The dengue (DEN) viruses are positive-strand RNA viruses in the genus ...Flavivirus. Dengue fever and dengue hemorrhagic fever/dengue shock syndrome are important human arboviral diseases ... See full document
7
Development and Evaluation of Serotype and Group Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus
... The results we obtained highlight the potential for the den- gue TaqMan assay as a rapid, specific, and sensitive tool for the epidemiological and diagnostic investigation of dengue virus. These assays have ... See full document
6
Detection of Dengue Virus Replication in Peripheral Blood Mononuclear Cells from Dengue Virus Type 2 Infected Patients by a Reverse Transcription Real Time PCR Assay
... Although dengue virus replicates in a variety of cells in vitro, including cells of the myeloid, lymphoid, epithelial, endothe- lial, and fibroblastic lineages, the cell types supporting dengue ... See full document
7
Rapid Detection of Classical Swine Fever Virus by a Portable Real Time Reverse Transcriptase PCR Assay
... outperformed virus isolation, which is considered the diagnostic standard for CSF (8, 24, ...before detection by virus isolation ...early detection of ...by virus ... See full document
6
Detection of Dengue Virus Genome in Urine by Real Time Reverse Transcriptase PCR: a Laboratory Diagnostic Method Useful after Disappearance of the Genome in Serum
... of dengue virus (DENV) infection has created a requirement for improved laboratory diagnostic ...genome detection in urine was evaluated as a diagnostic ...time reverse transcriptase ... See full document
6
Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... When virus detection fails, clinicians usually resort to serol- ogy tests for the detection of specific antibodies to dengue ...serological detection methods have been de- scribed (1, ... See full document
7
Rapid Detection of West Nile Virus from Human Clinical Specimens, Field Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase PCR Assay
... assay. Virus isolation in cell culture from CSF, serum, or tissues, followed by virus identification in an immunofluorescence assay with WN virus-specific monoclonal antibodies can also yield ... See full document
6
Detection and Serotyping of Dengue Virus in Serum Samples by Multiplex Reverse Transcriptase PCR Ligase Detection Reaction Assay
... scanned using a Pro-Scan array (Perkin-Elmer, Wellesley, ...of detection (LOD), 10-fold serial dilutions of viral culture stocks were prepared for all four DENV serotypes from standard stock cultures, with ... See full document
9
Development of an Internally Controlled Real Time Reverse Transcriptase PCR Assay for Pan Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays
... A further benefit of the pan-DENV assay is the multiplex de- tection of RNase P, which serves as a heterologous intrinsic IC. Of the method comparisons mentioned previously, only the conven- tional RT-PCR ... See full document
10
Molecular Typing of Imported Dengue Virus (DENV) Isolates from Patients in the Far East of Russia
... Severe Dengue is associated with plasma leakage, bleeding and ...DENV isolation, as well as detection of viral RNA, antigens and/or antibodies used singly or in ...are virus isolation ... See full document
5
Hepatitis C Virus Subtyping by a Core Envelope 1 Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish Patients
... Hepatitis C virus (HCV) is an important cause of chronic liver disease. HCV causes 20% of acute hepatitis cases, 70% of all chronic hepatitis cases, 40% of all cases of cirrhosis of the liver, 60% of ... See full document
10
Typing and Subtyping Influenza Virus Using DNA Microarrays and Multiplex Reverse Transcriptase PCR
... surface using a 5⬘- amine-tagged oligonucleotide and carbodiimide ...reamplified using a single pair of primers directed against flanking vector ...B virus strains, we designed 52 primers ... See full document
9
Immunological Relationships of Reverse Transcriptases from Ribonucleic Acid Tumor Viruses
... Since antisera to the R-MuLV reverse transcriptase were equally potent whether tested against disrupted whole virus or partially purified enzyme, lack of inhibition of the reverse transc[r] ... See full document
6
Detection of Marek's Disease Virus DNA in Chicken but Not in Human Plasma
... on an epidemiological study which showed an unusually high prevalence of MS in Key West, Fla. (3). Then it was postulated that seabirds were likely vectors of an environmental pathogen which could have caused this ... See full document
5
Müller, Tara Catharina (2013): Non-malaria febrile illness: a cross-sectional, observational study in rural areas of Cambodia. Dissertation, LMU München: Medizinische Fakultät
... Male and female persons who visited the 3 health centers during the study from January 2008 to December 2010 were eligible to participate in the study if they were between 7 and 49 years old on the day of recruitment The ... See full document
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