[PDF] Top 20 Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C.

... We would expect such a polypeptide to migrate with an apparent size of 18,000 to 22,000 daltons, but no product this size is obviously apparent in the in vitro translation of the mix of [r] ... See full document

... RNA transfer Northern blot analysis with hybridization probes from the region of the herpes simplex virus type 1 DNA known to encode the gC gene indicated that gC mRNA was produced in MP[r] ... See full document

... recombinant virus, incubated at 37°C, harvested at 18 h after infection, solubilized in disruption buffer, electrophoretically separated in a denaturing polyacrylamide gel, transferred to a nitrocel- lulose ... See full document

... the second, a mixture of Bac-gD-A and Bac-gD-D failed to block apoptosis. The data lend themselves to three conclu- sions. The first conclusion is that all three domains of gD, the ectodomain, transmembrane domain, and ... See full document

... To obtain the complete nucleotide sequence of the region of the HSV-2 genome encoding gC, the protein encoded by the smaller 3'coterminal message, and the upstream and downstream regulat[r] ... See full document

... substantial portion of gK is predicted to lie within the lumen of cellular organelles that is functionally equivalent to the outside of the ...amino-terminal portion of gK terminating with the last amino ... See full document

... HSV-1 genome into a hot spot for homologous ...and c sequences which normally flank the a sequence in the HSV-1 genome ...a virus in this study, whose L-S junctions lacked a ... See full document

... not detailed in this ...that analysis of se- quence characteristics gives a clear positive evaluation of cod- ing potential, but a negative indication must in principle be less than ...perimental ... See full document

... secreted portion of glycoprotein G (sgG-2) of herpes simplex virus type 2 (HSV-2) was evaluated as a novel antigen in an enzyme-linked immunosorbent assay (ELISA) format for ... See full document

... HSV-1 recombinants containing chimeric gC genes composed of HSV-1 and HSV-2 sequences were used to localize neomycin resistance within the N-terminal 223 amino acids of gC-2.. This regio[r] ... See full document

... To fine map amino acid residues which are recognized by site I type-specific MAbs, point mutations were introduced into site I of the gC-1 or gC-2 gene, which resulted in recombinant mut[r] ... See full document

... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document

... However, a search for homology at the amino acid level showed regions of significant sequence similarity between the amino acids of the carboxy half of EHV-1 gp13 and those of the same r[r] ... See full document

... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document

... & C to human neuroblastoma cells ...& C and cells were treated with cross-linker at various times after the temperature ...in virus alone, the length and concentration of the cross-linking ... See full document

... Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus.. Potent neutr[r] ... See full document

... Us9null virus failed to spread in an antero- grade direction to visual centers in the brain (26, ...Us9null virus had no antigen at all in the brain, while 20% had limited antigen in the suprachiasmatic ... See full document

... With these problems in mind, we examined the vaccine potential of a fully disabled herpes simplex virus type 1 mutant that is capable of only a single round of replication, since a virus[r] ... See full document

... gE380 virus, a rescued virus was constructed to further confirm the importance of the gE380 region in ...rescued virus was comparable to that of wild-type virus when evaluated by ... See full document

... The cloned fragments were identified by digestion of the chimeric plasmid DNA with restriction enzymes EcoRI or EcoRI and BglII followed by comparison of their electrophoretic mobilities[r] ... See full document