[PDF] Top 20 Detection and characterization of multiple forms of simian virus 40 large T antigen.
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Detection and characterization of multiple forms of simian virus 40 large T antigen.
... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... The T antigen OBD (amino acids 131 to 260) was purified from bacteria as a double fusion with glutathione S-transferase (GST) and a hexahistidine tag ...polyhistidine-tagged T antigen OBD ... See full document
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Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... p53-independent forms of apoptosis appear to function in many ...reference 40 for a review); however, it is becoming increasingly clear that p53-indepen- dent mechanisms of adenovirus-induced apoptosis also ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... including simian virus 40 (SV40) and papillomavirus (reviewed in reference ...contain multiple initi- ator binding sites that consist of short sequences of 5 or 6 base pairs organized as pairs ... See full document
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Immunoprecipitation of some forms of simian virus 40 large-T antigen by antibodies to synthetic peptides.
... Because during the course of these and similar studies 16, 17; unpublished data we found little correlation between the activity of a given serum against its peptide and the correspondin[r] ... See full document
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Monoclonal antibodies specific for the carboxy terminus of simian virus 40 large T antigen.
... Peptides used for immunization of mice and in characterization of antibodies Designation Amino acid sequences corresponding to Tthe of SV40 large carboxy-terminal region Lys-Thrz Hexamer[r] ... See full document
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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Altered phosphorylation of free and bound forms of monkey p53 and simian virus 40 large T antigen during lytic infection.
... 1312 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest We have identified the phosphorylation sites in monkey p53 as well as specific changes in the phosphorylation state [r] ... See full document
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Linker insertion mutants of simian virus 40 large T antigen that show trans-dominant interference with wild-type large T antigen map to multiple sites within the T-antigen gene.
... It also plays central roles in immortalization of primary cells 61, 62 and in the malignant Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Linker insertion mutants aff[r] ... See full document
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... performs multiple functions during replication. A well-studied example is large T antigen (T-ag), the initiator protein from simian virus 40 ...of T-ag that ... See full document
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Different forms of simian virus 40 large tumor antigen varying in their affinities for DNA.
... We can speculate that, although the newly synthesized 5 to 7S T antigen form is the active viral origin-binding form, the subsequently formed more rapidly sedimenting T antigen oligomers[r] ... See full document
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Simian Virus 40 Large T Antigen Is Phosphorylated at Multiple Sites Clustered in Two Separate Regions
... The lower part shows the carboxy-terminal region downstream from Phe617 with the extensions of the phosphopeptides that can be generated by digestion with trypsin or with chymotrypsin as[r] ... See full document
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document
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The zinc finger region of simian virus 40 large T antigen.
... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ... See full document
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Functional implications of oligomerization of simian virus 40 large T antigen during lytic virus infection.
... To determine the distribution of various forms of T antigen on the sucrose density gradients at a quantitative level, the corresponding gel bands were excised, eluted with 0.2 ml of 60% [r] ... See full document
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Multiple DNA Damage Signaling and Repair Pathways Deregulated by Simian Virus 40 Large T Antigen
... in large nuclear foci ...the large amounts of unusual replication struc- tures (theta-type intermediates, for example) that are pro- duced during an SV40 infection and that are predicted to initiate DDR and ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... SV40 large-T antigen might be me- diated by the mRNA that encodes the large-T antigen, rather than by the ...mutant large-T plasmid (pR137) was constructed in which ... See full document
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Simian virus 40 large T antigen oligomers: analysis of electrophoresis in the absence of detergent.
... 711-716 0022-538X/85/120711-06$02.00/0 Copyright © 1985, American Society for Microbiology Simian Virus 40 Large T Antigen Oligomers: Analysis of Electrophoresis in the Absence of Deterg[r] ... See full document
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Identification of a region of simian virus 40 large T antigen required for cell transformation.
... However, mutants which lack origin-binding activity retain the ability to transform both primary and established cells phenotypically, indicating that It has been known for some time tha[r] ... See full document
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