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Detection of herpes simplex virus type 1 transcripts during latent infection in mice.
... Total cellular RNAs were isolated from the trigeminal ganglia of acutely 5 days postinfection and latently infected mice, Northern blotted 5 ,g per lane, and hybridized to a nick-transla[r] ... See full document
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The herpes simplex virus type 1 immediate-early protein ICP0 is necessary for the efficient establishment of latent infection.
... productive infection, at early times after infection, ICP0 transiently localizes to discrete subnuclear structures, known as ND10 or PODs, and as a consequence the cellular ND10 antigens are dispersed (11, ... See full document
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Gender Influences Herpes Simplex Virus Type 1 Infection in Normal and Gamma Interferon-Mutant Mice
... ganglionic infection or spontaneous reactivation of HSV-1 did not occur in 129/Sv//Ev or GKO mice ...control mice were subjected to hyperthermic stress at 30 to 60 days postinfection, and ... See full document
6
Hematogenous Vertical Transmission of Herpes Simplex Virus Type 1 in Mice
... Gender-dependent infection was observed both in neonates and adults, with females showing significantly higher levels of viral DNA and proteins in the encephalon. This confirms our previous findings (6). The ... See full document
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Expression of herpes simplex virus type 1 latency-associated transcripts in the trigeminal ganglia of mice during acute infection and reactivation of latent infection.
... These data demonstrate that i during an acute infection the transcripts of a number of HSV-1 genes involved in the lytic replication cycle rapidly increase and decrease over a 7-day peri[r] ... See full document
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Herpes Simplex Virus Type 2 (HSV-2) Establishes Latent Infection in a Different Population of Ganglionic Neurons than HSV-1: Role of Latency-Associated Transcripts
... of virus type-associated, site-specific recurrence ...deletion virus that expresses the lacZ gene under control of the HSV-1 LAT ...this virus preferentially established latency (as ... See full document
7
Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear.
... latency-associated transcripts (LATs) of herpes simplex virus type 1 (HSV-1) are the only viral gene products that accumulate to abundant levels in latently infected ... See full document
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Detection and preliminary characterization of herpes simplex virus type 1 transcripts in latently infected human trigeminal ganglia.
... In situ hybridizations of human trigeminal ganglia using probes homologous to the BamHI SP region detected HSV-1 RNA transcribed from the same strand as the 1.85- and 1.35-kb latency tra[r] ... See full document
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Detection of multiple strains of latent herpes simplex virus type 1 within individual human hosts.
... Restriction endonuclease analysis of DNAs of latent HSV type 1 HSV-1 recovered from human cadavers has shown that isolates from the ganglia of different individuals can be distinguished [r] ... See full document
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Interleukin-18 Protects Mice against Acute Herpes Simplex Virus Type 1 Infection
... from mice treated with ...from mice treated with IL-18 are ongoing, and at least two possibilities are being considered: a change in the IFN-g-pro- ducing cell population of peritoneal cells or a change in ... See full document
9
Noninvasive Bioluminescence Imaging of Herpes Simplex Virus Type 1 Infection and Therapy in Living Mice
... of herpes simplex virus type 1 (HSV-1) infection provide significant insights into viral and host genes that regulate disease pathogenesis, but conventional methods to ... See full document
13
Genetic Studies Exposing the Splicing Events Involved in Herpes Simplex Virus Type 1 Latency-Associated Transcript Production during Lytic and Latent Infection
... or latent KOS infections, showed the joining of upstream and downstream sequences precisely at the predicted exon boundaries ...and latent infection, which supports the interpretation of our ... See full document
11
Herpes simplex virus type 1 protein IE63 affects the nuclear export of virus intron-containing transcripts.
... taining transcripts. The distribution of IE110 transcripts in cells infected with the mutant virus 110C1, in which both in- trons of IE110 have been precisely removed, indicates that exonic sequences ... See full document
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Detection of Herpes Simplex Virus Infection in Cerebrospinal Fluid
... Herpes simplex virus (HSV) is one of the main causes of viral infections of the central nervous system ...the virus reaches peripheral ganglions and then CNS through axonal route and causes ... See full document
5
During Herpes Simplex Virus Type 1 Infection of Rabbits, the Ability To Express the Latency-Associated Transcript Increases Latent-Phase Transcription of Lytic Genes
... for mice infected with a wild-type virus, the HSV-1 genome’s lytic gene promot- ers become associated with dimethyl histone H3 K9, a marker of transcriptional repression, during ... See full document
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Visualization by Live-Cell Microscopy of Disruption of ND10 during Herpes Simplex Virus Type 1 Infection
... the infection period, there was a dramatic reorganization of the PML protein followed by its accumulation into a reduced num- ber of foci ...after infection many of the PML foci become surrounded by ... See full document
5
Modification and Reorganization of the Cytoprotective Cellular Chaperone Hsp27 during Herpes Simplex Virus Type 1 Infection
... points during the course of ...the virus. It is also possible that Hsp27 mod- ification during HSV-1 infection is related to a newly described role for the protein in ARE (AU-rich RNA ... See full document
9
Impact of Rac1 and Cdc42 Signaling during Early Herpes Simplex Virus Type 1 Infection of Keratinocytes
... knockout mice has provided a new tool for studying Rac1 function in vivo (20), in particular since a general Rac1 knockout causes lethality early in embryogenesis ...by infection, we observed ICP0 ... See full document
14
A herpes simplex virus type 1 latency-associated transcript mutant reactivates with normal kinetics from latent infection.
... Expression of herpes simplex virus type 1 latency-associated transcripts in the trigeminal ganglia of mice during acute infection and reactivation of latent infection.. Expression of her[r] ... See full document
10
The abundant latency-associated transcripts of herpes simplex virus type 1 are bound to polyribosomes in cultured neuronal cells and during latent infection in mouse trigeminal ganglia.
... A 299-bp DNA fragment representative of the unspliced 2.0-kb LAT, produced by primers P5 and P3, was detected in total RNA from NA4 cells (Fig. 3A, lane 2) as well as in all five separate polysomal fractions of RNA from ... See full document
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