[PDF] Top 20 Detection and identification of mycobacteria by amplification of rRNA
Has 10000 "Detection and identification of mycobacteria by amplification of rRNA" found on our website. Below are the top 20 most common "Detection and identification of mycobacteria by amplification of rRNA".
Detection and identification of mycobacteria by amplification of rRNA
... Hybridization of nucleic acids 1 ,ug from the indicated bacterial species to an oligonucleotide broadly reactive for mycobacteria at the genus level reverse 247, with sequence lT- CAC GA[r] ... See full document
9
Detection and identification of mycobacteria by DNA amplification and oligonucleotide specific capture plate hybridization
... Nontuberculous mycobacteria (NTM) are also increasing in importance (12, 14, ...rapid identification of mycobacteria extremely ...for identification are based on morphological, biochemical, ... See full document
5
Detection and identification of mycobacteria by amplification of RNA and DNA in pretreated blood and bone marrow aspirates by a simple lysis method
... two amplification procedures (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test [AMTDT] and Roche Amplicor ...the detection and iden- tification of Mycobacterium tuberculosis and ...on ... See full document
5
DNA amplification and reverse dot blot hybridization for detection and identification of mycobacteria to the species level in the clinical laboratory
... DNA Amplification and Reverse Dot Blot Hybridization for Detection and Identification of Mycobacteria to the Species Level in the Clinical Laboratory ELLEN H.. BROOKS' Microbiology Secti[r] ... See full document
5
PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples
... the detection and identification of mycobacteria in clinical ...16S rRNA with a set of primers that is specific for the genus Mycobacterium and that flanks species-specific sequences within ... See full document
9
Novel Diagnostic Algorithm for Identification of Mycobacteria Using Genus Specific Amplification of the 16S 23S rRNA Gene Spacer and Restriction Endonucleases
... growing mycobacteria are still very incomplete (21, 26), it appears mandatory to look for the possibility that additional RFLP genotypes found may represent unknown infrasubspe- cific 16S rDNA ... See full document
11
Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... strain identification includes analysis of the gene encoding 16S rRNA ...the identification of mycobac- teria by PCR-based ...16S rRNA sequence determination is not only more rapid but also ... See full document
7
Direct Detection of Mycobacterial Species in Pulmonary Specimens by Two Rapid Amplification Tests, the Gen Probe Amplified Mycobacterium tuberculosis Direct Test and the GenoType Mycobacteria Direct Test
... 16S rRNA gene, was used as the reference ...for detection of the MTC, ...using identification of cultured isolates as the reference ...atypical mycobacteria that the GTMD test failed to ... See full document
5
Evaluation of the LiPA MYCOBACTERIA Assay for Identification of Mycobacterial Species from BACTEC 12B Bottles
... LiPA MYCOBACTERIA (Innogenetics NV, Ghent, Belgium) assay was used to identify mycobacterial isolates using culture fluid from positive BACTEC 12B ...23S rRNA spacer region, to oligonucleotide probes ... See full document
5
Accelerated Detection and Identification of Mycobacteria with MGIT 960 and COBAS AMPLICOR Systems
... for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Lo¨wenstein-Jensen (L-J) ...of mycobacteria other than ...to detection of ...to ... See full document
5
Detection and Identification of Mycobacterium spp in Clinical Specimens by Combining the Roche Cobas Amplicor Mycobacterium tuberculosis Assay with Mycobacterium Genus Detection and Nucleic Acid Sequencing
... for detection and identifi- cation of Mycobacterium ...16S rRNA gene amplification of nucleic acids extracted from the clinical sam- ple, followed by sequence analysis, facilitated rapid identifica- ... See full document
6
Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene
... Members of the cell wall-deficient class Mollicutes are the smallest self-replicating organisms known (12). They possess the smallest genome of any free-living organism, and the G 1 C content of their DNA is among the ... See full document
5
Identification and Detection ofStenotrophomonas maltophilia by rRNA Directed PCR
... secretions. Identification of ...definitive identification, we developed species- specific PCR (SS-PCR) primers, designated SM1 and SM4, directed to the 23S rRNA gene, and tested their utility to ... See full document
5
PCR enzyme linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria
... for amplification and rapid identification of myco- bacterial DNA coding for 16S rRNA was ...16S rRNA gene from all mycobacteria while simultaneously labelling one strand of the ... See full document
6
Detection and identification of mycobacteria in sputum from suspected tuberculosis patients
... in detecting Salmonella typhi in blood, feces and urine from suspect typhoid fever and multiplex PCR to detect M. tuberculosis complex bacteria and other mycobac- teria which this technique is based on the ... See full document
6
Identification of a Novel Conjugative Plasmid in Mycobacteria That Requires Both Type IV and Type VII Secretion
... esx-P1locus (see Table S2 in the supplemental material), targeting the conserved eccC or eccA homologues. As expected, we could amplify a DNA fragment of the expected size with all primer pairs for E11, whereas the ... See full document
8
Molecular Detection of Some Virulence Traits Among Pseudomonas aeruginosa Isolates, Hilla-Iraq
... Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and ... See full document
8
Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
... When crude nucleic acid extracts prepared from two of the above culture-positive gastric biopsy specimen homogenates were used as templates in RT-PCR, the expected amplified fragment fro[r] ... See full document
7
Isolation and Molecular characterization of Cellulase Producing Bacteria from Soil
... Screenings of bacteria for their cellulase activity were carried out by the hydrolysis of substrate incorporating in the basal salt medium. After an incubation period, enzyme activities were detected by the appearance of ... See full document
5
Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection.
... We report here the application of recently developed nucleic acid amplification and oligonucleotidebased detection procedures for the identification of HIV sequences in established infec[r] ... See full document
5
Related subjects