[PDF] Top 20 Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification

... or pathogen DNA, and (ii) a conjugation chemistry that bonds the DNA element to the polypeptide without inhibiting IgG-binding activity or interfering with the ability to promote ... See full document

... lar detection assays specific for this virus would be in great ...transcription–loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of the ... See full document

... Standard plasmid preparation. To compare the sensitivities of the ALV-J LAMP assay and conventional PCR, a 545-bp fragment comprising positions 5258 to 5802 of the genome sequence of the NX0101 isolate was amplified by ... See full document

... acid amplification platforms for tuberculosis (TB) detection are not designed to be simple or inex- pensive enough to implement in decentralized settings in countries with a high burden of ...The ... See full document

... The loop-mediated isothermal amplification (LAMP) technique was invented in 2000 [17] and provides a power- ful gene amplification tool with simple conditions requiring only a water ... See full document

... Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification method that allows reactions to occur under isothermal conditions [18]; the Bst DNA polymerase can ... See full document

... equipment. Loop mediated isothermal amplification technique (LAMP) is a technique developed by Notomi et ...under isothermal conditions. This nucleic acid amplification technique ... See full document

... the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African ...The ... See full document

... [2]. Detection of these infections relies on more sensitive methods, such as polymerase chain reaction (PCR), which have a detec- tion limit of 1–2 parasites/µL when extracted from a dry blood spot (DBS) [4, 6, ... See full document

... the loop-mediated isothermal amplification (LAMP) assay for the rapid, simple, and sensitive detection of Newcastle disease virus (NDV) directly from culture isolates as well as ... See full document

... a loop-mediated isothermal amplification (LAMP) assay for rapid detection of ...highly specific for CaPVs, with no apparent cross-reactivity to other related viruses (near ... See full document

... (loop mediated isothermal amplification) technique utilizes Bst DNA polymerase to create stand- displacement amplification, and operates at a constant temperature ...the ... See full document

... Loop-mediated isothermal amplification (LAMP), the novel method developed by Notomi et al [4], is able to amplify DNA with great efficacy as its detection limit for DNA in the reaction ... See full document

... In the present study, we successfully designed a LAMP assay targeting a conserved locus of Y.entero- colitica. This bacterium has many genes involved in pathogenicity, but there are limited studies targeting the ... See full document

... Theoretically, Bst polymerase used in LAMP has a higher tolerance of inhibitors than Taq polymerase used in qPCR [63, 64]. This suggests that the sensitivity of LAMP is slightly higher than that of qPCR, which is use- ... See full document

... In the present study, three methods were used for detection of Legionella in patients with pneumonia symptoms. We demonstrated that the LAMP assay is a potential diagnostic tool for L. pneumophila detection ... See full document

... replication and several distinct stages, most of which contain ~14 or more genomes when mature, suggest such a sensitivity range is appropriate [35]. Indeed, LAMP was found to be a more sensitive diagnostic of eimerian ... See full document

... molecular detection methods, the ad- vantage of the RT-LAMP assay is due to continuous am- plification under isothermal conditions using six primers that recognize eight distinct regions of the target gene ... See full document

... The effort is, nowadays, in fabricating cheap port- able instruments for the use of LAMP in remote areas or third world countries, as commercial instruments are too expensive, not portable or difficult to be operated by ... See full document

... assay detection limits. The detection of HHV-7 LAMP products by agarose gel electrophoresis gave a sensitivity of 250 copies/tube for a 30-min reaction ...3). Detection by the turbidity assay, ... See full document