[PDF] Top 20 Development of a PCR Based Line Probe Assay for Identification of Fungal Pathogens
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Development of a PCR Based Line Probe Assay for Identification of Fungal Pathogens
... reverse-hybridization line probe assay (LiPA) which when combined with PCR amplification detects and identifies clinically significant fungal pathogens including Candida, ... See full document
8
Development of a DNA Microarray for Detection and Identification of Fungal Pathogens Involved in Invasive Mycoses
... of identification of pathogenic fungi used in clinical microbi- ology is based on phenotypic features and physiological tests and is therefore ...of fungal pathogens. In view of the increasing ... See full document
11
Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... In fungal infections, even when positive, results usually take longer than a week because these organisms are difficult to identify and/or are ...the development of culture-independent diag- nostic tests ... See full document
7
Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues
... metagenomic assay to detect small nonhuman genomes in an overwhelming background of human sequences will be affected by several factors, including nucleic acid extraction and recovery, target size and copy number, ... See full document
13
Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... initial development in 2006, RPA technology has been successfully used for rapid detection of vari- ous pathogens ...cent probe-based RPA assay has been developed and evaluated for ... See full document
7
Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
... Twenty sets of serotype-specific primer pairs and probes were designed; the remaining 30 serotypes shared identical or very similar wzy sequences with one or two others in the same or closely related serogroups, as ... See full document
5
Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide Resistant Mycobacterium tuberculosis Strains
... LiPA. The LiPA described here was developed on the basis of the same principle as that of the commercially available INNO-LiPA Rif. TB kit (Inno- genetics, Ghent, Belgium) for the detection of rifampin resistance (14). ... See full document
6
Quantitative Multiprobe PCR Assay for Simultaneous Detection and Identification to Species Level of Bacterial Pathogens
... decreased PCR sensitivity ...the PCR amplification system. The quantitative capacity of real-time PCR has thus rede- fined the standards by which a decontamination method is ...precise ... See full document
6
Direct Application of the INNO LiPA Rif TB Line Probe Assay for Rapid Identification of Mycobacterium tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear Positive Respiratory Specimens from an Area of High Incidence of Multidrug Resistant Tuberculosis
... Rif.TB assay as carrying the following mutations: S531L (29 isolates), H526Y, and D516V (1 isolate ...Rif.TB assay failed to detect any mutation in one sample that was repeatedly identified as RIF resistant ... See full document
5
Performance Assessment of New Multiplex Probe Assay for Identification of Mycobacteria
... color development of the strip was carried out at room temperature on a shaking platform following two washes with 2 ml of rinse solution, the addition of 2 ml of conjugate (streptavidin labeled with alkaline ... See full document
6
Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels
... a PCR-based assay which allows the detection of staphylococci at the genus level by targeting the tuf gene, which encodes the elongation factor ...Degenerate PCR primers derived from con- ... See full document
7
Development and Clinical Evaluation of a Highly Sensitive PCR Reverse Hybridization Line Probe Assay for Detection and Identification of Anogenital Human Papillomavirus
... Human papillomaviruses (HPV) constitute a group of vi- ruses associated with benign and malignant lesions of cutane- ous and mucosal epithelia. So far, more than 100 different HPV genotypes have been identified, of which ... See full document
10
PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis
... as PCR, real-time PCR, and microarray analyses, are used to detect pathogens, and these methods have shown enhanced sensitivities and specificities for microbial pathogens ...new assay ... See full document
7
Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... the PCR products, a time consuming process. A first generation line-probe assay was created for genotyping NoV based on the highly conserved ORF1-ORF2 ...The assay was vali- ... See full document
8
Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... of fungal DNA from various clinical samples has been shown to be highly ...real-time PCR and direct sequencing of positive PCR products improves the di- agnostic turnaround ...of fungal ... See full document
8
Utility of a Commercially Available Multiplex Real Time PCR Assay To Detect Bacterial and Fungal Pathogens in Febrile Neutropenia
... by PCR in our study could well be caused by the tech- ...our PCR results strongly suggest that a blood culture prior to antibiotic therapy in febrile neutropenia cannot be replaced by a molecular method ... See full document
6
Development of a 16S rRNA PCR RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections
... the PCR-RFLP identification This rapid technique may be of special interest to describe more in depth the real role of different Bartonella ...identifications based on clinical findings may not be ... See full document
8
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... the PCR products and a resulting decrease in SYBR green fluorescence as the dye is ...this assay in the species identification trial (Table 2) where the MCA exhibited a low failure rate but ... See full document
10
Use of GeneXpert Remnants for Drug Resistance Profiling and Molecular Epidemiology of Tuberculosis in Libreville, Gabon
... as confirmed in this study, is a reliable indicator of MDR-TB (21). However, most methods for detecting resistance to antibiotics other than rifampin require culturing of the specimen. Cultures on solid media take more ... See full document
11
Diagnosis of Prosthetic Joint Infection by Use of PCR Electrospray Ionization Mass Spectrometry
... Multiplex PCR of sonication fluid accurately differentiates between prosthetic joint infection and aseptic ...Successful identification of pathogens by polymerase chain reaction ... See full document
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