[PDF] Top 20 Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
Has 10000 "Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses" found on our website. Below are the top 20 most common "Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses".
Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
... of dengue viruses ...four tube based RT-PCR followed by nested PCR or four serotype specific ...plex PCR assay described by Harris et ...proper evaluation with ... See full document
5
Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... replace PCR because of its simplicity, rapidity, specificity, and cost- ...titative detection of gene amplification is possible by real-time monitoring of turbidity in an inexpensive turbidimeter ...the ... See full document
7
Development and evaluation of a one step multiplex real time TaqMan® RT qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples
... first one- step multiplex TaqMan ® real-time reverse transcription polymerase chain reaction (RT-qPCR) assay targeting the NSP3 and VP7 genes of ERVA G3 and G14 genotypes for the rapid ... See full document
11
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... transcriptase PCR (RT- PCR), and serology (immunoglobulin M [IgM] and IgG enzyme- linked immunosorbent assays [ELISAs]) ...by PCR is the most rapid diagnostic ...several ... See full document
8
Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... A multiplex reverse transcription-PCR (RT-PCR) assay was developed to allow in one test the detection of nine different microorganisms (enterovirus, influenza A and B ... See full document
7
Evaluation of four commercial real time RT PCR kits for the detection of dengue viruses in clinical samples
... next-day detection of DENV in acute- phase serum or plasma. The 2-step hemi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) proto- col, originally reported by Lanciotti et ...a ... See full document
5
Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... immunologic detection tests. We have reported the development of three assays that we had used during the 2005 dengue outbreak in Singapore in support of the diagnos- tic and surveillance ... See full document
7
Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... Multiplex PCR assays capable of amplifying 13 or more separate regions of chromosomal DNA and of detecting and typing several bacterial and viral pathogens have also been described (3; reviewed in ... See full document
7
Evaluation of custom multiplex real time RT PCR in comparison to fast track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses
... simultaneous detection of three dif- ferent viruses in a single reaction ...three viruses. Tube-1 [Influenza B (Flu B), human Coronaviruses229E (HCoV 229E), OC43 (HCoV-OC43)], ... See full document
7
Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single Tube Multiplex Reverse Transcriptase PCR
... the detection of dengue virus in infected mosquitoes without RNA degradation or PCR ...a single amplification reaction ...inhibitors. RT-PCR conducted with RNA extracted by this ... See full document
6
Standardization of one step single tube rt pcr for rapid detection of all circulating strains of dengue viruses
... of dengue are very similar with other infections therefore disease can be confirmed with antigenic or immunologic ...Presently dengue is diagnosed by virus isolation, antigen detection, ELISA, ... See full document
5
Development and Validation of Multiplex One Step Real Time TaqManqRT PCR Assays for Detection and Quantification of Arboviral Encephalitis Viruses
... controlled one-step real-time qRT-PCR assays for multiplex detection of 15 viruses, which covered nearly all the important viral pathogens that cause arboviral encephalitis, in- ... See full document
39
The development of a rapid SYBR one step real time RT PCR for detection of porcine reproductive and respiratory syndrome virus
... Therefore, rapid, specific and sensitive assays are required for diagnosis of PRRSV in ...the rapid and specific detection of PRRSV in pigs; however, these conventional RT-PCR assays ... See full document
7
Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... Sensitivity and specificity. The L segment of the RVFV genome was used to design primers, as it encodes the viral polymerase and was thus more prone to contain conserved sequences than the S and M segments. We initially ... See full document
7
Molecular Typing of Imported Dengue Virus (DENV) Isolates from Patients in the Far East of Russia
... Severe Dengue is associated with plasma leakage, bleeding and ...as detection of viral RNA, antigens and/or antibodies used singly or in ...with PCR that result in a confirmed diagnosis of an acute ... See full document
5
Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one step real time RT PCR assay
... the multiplex assay and the WHO-recommended assays did not significantly differ in their ability to detect H7N9 viruses; only one positive fecal sample detected by the multiplex assay was ... See full document
9
Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... three viruses were detected well by Pan-Flavi assay (Figure ...a single probe for detection of all flaviviruses is challenging because of the genetic diversity and the restricted length of conserved ... See full document
11
Simultaneous Detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in Suspected Cases of Meningitis and Septicemia Using Real Time PCR
... Sequence Detection System (ABI, Warrington, United Kingdom), known as TaqMan, enables amplification and detection to be carried out at the same time in a closed-tube ...real-time PCR ... See full document
6
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... Primers and Probes were designed based on the highly conservative regions of HA and NA genes of A/Duck/ Guangxi/1/18(H5N6) virus downloaded from GenBank by using the MegAlign module of the DNAStar package. One H5 ... See full document
7
One step real time RT PCR assays for serotyping dengue virus in clinical samples
... A sample was classified as positive in the CDC DENV-1-4 real-time RT-PCR assays if the amplification curve crossed the threshold line within 37 cycles Five of the six DENV1 RT-PCR positi[r] ... See full document
7
Related subjects