[PDF] Top 20 Development of a real time quantitative PCR assay for detection of a stable genomic region of BK virus
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Development of a real time quantitative PCR assay for detection of a stable genomic region of BK virus
... for assay devel- opment might be expected to result in lack of specifi- ...the stable region to design an assay specific for ...meet assay specificity requirements by utilizing a ... See full document
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Development and application of SYBR Green I real time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B
... of virus distribution and load of ALV-J in different organs in each group at 30 weeks post-infection by p27 antigen de- tection, routine PCR and real-time PCR ...5, virus copies ... See full document
10
Development and Validation of a Quantitative Real Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis
... of assay format, primers, and probes warrant ...nested PCR approach as a method to increase the sensitivity of the assay because of the major difficulties of amplicon contamination inherent in such a ... See full document
7
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... Primers and Probes were designed based on the highly conservative regions of HA and NA genes of A/Duck/ Guangxi/1/18(H5N6) virus downloaded from GenBank by using the MegAlign module of the DNAStar package. One H5 ... See full document
7
Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... respective genomic and plasmid DNA types, which does not always hold true ...from real-time PCR quantification on true bio- logical samples, qPCR results were compared with those obtained from ... See full document
8
Quantitation of Hepatitis B Virus Genomic DNA by Real Time Detection PCR
... serum virus levels frequently induce fluctuation of serum ALT ...serum virus level is useful in monitoring the clinical course of patients with active hepatitis ...reliable quantitative assay ... See full document
5
SYBR Green Based Real Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False Negative Results Due to Strain Variability
... Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus ...on assay performance, we generated every possible ... See full document
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Monitoring of Polyomavirus BK Virus Viruria and Viremia in Renal Allograft Recipients by Use of a Quantitative Real Time PCR Assay: One Year Prospective Study
... as detection of the presence of decoy cells in the urine and detection of BKV in the urine by electron microscopy (EM) or conventional PCR, is limited because of the ubiquitous nature of the ... See full document
6
Comparative evaluation of a laboratory developed real time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
... testing genomic DNA/RNA extracted from other viruses causing similar symptoms to adenovirus ...qPCR assay was evaluated by analyzing a dilution series of HAdV-negative plasma and VTM matrices spiked with ... See full document
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... ompA-based real-time PCR as well as both of the 16S rRNA-based conven- tional assays detected ...the PCR of Tong and Sillis (24) were posi- ...single-step PCR did not detect its target ... See full document
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A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... revealed stable repeatability and a sensi- tivity of 100 copies/reaction and no cross reaction with other common respiratory viruses or HAdV serotypes 1,4,5,6,31 and ...nested PCR, we speculated that these ... See full document
6
Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... of detection was ⱖ 5 copies per ...this detection limit estimate was documented by the intra-assay reproducibility test, where nine replicates of 1 copy/ l were ...the PCR is ex- pected to ... See full document
8
Applicability of a Real Time Quantitative PCR Assay for Diagnosis of Respiratory Syncytial Virus Infection in Immunocompromised Adults
... conserved genomic regions of the N ...universal PCR master mix (PE Applied Biosys- tems), 900 nM concentrations of each forward primer, 900 nM concentrations of the reverse primers, and 200 nM ... See full document
5
Comparison of real time PCR and microscopy for malaria parasite detection in Malawian pregnant women
... pfldh real-time PCR assay we employed is highly sensitive and specific for ...general, real-time PCR assays offer specific advantages over other PCR-based chemis- ... See full document
9
Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay
... tected PCR inhibitors and estimated the efficiency of the ex- traction methods, both of which are particularly important to quantify the EBV load accurately and ...conventional PCR assays, which have ... See full document
5
A Quick and Efficient Method to Quantify Baculo Virus by Quantitative Real Time PCR
... µL virus solutions was removed from the viral stock to be titrated (P3 Virus stock) and used for DNA ...a Genomic DNA isolation Kit (Qiagen-India) as described by the manufacturer’s ...of ... See full document
5
Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... H1N1 virus pandemic, the detection of influenza virus types A and B and differentiation between influ- enza virus A subtypes became important for monitoring the out- ...influenza virus ... See full document
6
Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... the assay, a species-specific region of the Plasmodium 18S (small subunit) rRNA gene was targeted, as this gene contains multiple copies dispersed throughout the Plas- modium genome ...The ... See full document
11
Real Time PCR Assay To Detect Smallpox Virus
... the PCR products by agarose gel electrophoresis after restriction endonuclease di- ...and time-con- suming post-PCR ...nuclease PCR or the TaqMan assay, first de- veloped by Holland et ... See full document
5
Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... broad-range real-time PCR was developed for the rapid detection of human pathogenic ...The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) ...in PCR-positive ... See full document
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