[PDF] Top 20 Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
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Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... all flaviviruses deposited in the NCBI database to verify their flavivirus-generic amplifi- cation ...15 reverse primers and another four reverse primers targeting different ...the detection ... See full document
11
Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
... Dengue cases are increasing over the years in many parts of the world. Now it is endemic in India, with circulation of multiple serotypes [7,8]. There is also an increased inci- dence of fatal DHF and DSS, which requires ... See full document
5
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... real-time, one-step, multiplex RT-PCR assay was developed for the detection and quantitation of DENV and CHIKV RNAs in cell culture supernatants or human ...Real-time PCR was performed ... See full document
8
Fast Duplex One Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses
... flaviviruses. This is of particular interest because these viruses can produce similar symptoms, and there is a risk of overlook- ing exotic WNV cases in an area where JEV is endemic. To date, a WNV outbreak has ... See full document
5
Standardization of one step single tube rt pcr for rapid detection of all circulating strains of dengue viruses
... more rapid assays are required for dengue identification. Real time reverse transcription polymerase chain reaction (RT-PCR) is a better choice of diagnosis but ...types; one ... See full document
5
Shedding and Reversion of Oral Polio Vaccine Type 3 in Mexican Vaccinees: Comparison of Mutant Analysis by PCR and Enzyme Cleavage to a Real Time PCR Assay
... the development of vaccine-associated paralytic poliomyelitis ...by PCR and enzyme cleavage (MAPREC). This involves conventional reverse transcription-PCR to detect OPV3, followed by a ... See full document
7
Reverse Transcription PCR Enzyme Linked Immunosorbent Assay for Rapid Detection and Differentiation of Alphavirus Infections
... the detection of alphaviral RNA by RT-PCR using two degenerate, genus-specific primers de- veloped previously ...ELISA step were added to increase sensitivity, and specificity, and to simplify ...of ... See full document
9
Development of One Step, Real Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
... Conventional RT-PCR screening of a collection of 342 respi- ratory samples from patients hospitalized with relatively severe respiratory tract infections revealed seven HCoV OC43-infected samples (2.0%) and ... See full document
5
Sensitive and Specific Quantitative Detection of Rotavirus A by One Step Real Time Reverse Transcription PCR Assay without Antecedent Double Stranded RNA Denaturation
... TC-3=), reverse primer NVP3-R1 (5=-GGT CAC ATA ACG CCC CTA TA-3=), and probe NVP3-Probe (5=-A 6-carboxyfluores- cein [FAM] TG AGC ACA ATA GTT black hole quencher 1 [BHQ1] AAA AGC TAA CAC TGT CAA-3=) were used ... See full document
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Early Detection of Acute Rhinovirus Infections by a Rapid Reverse Transcription PCR Assay
... additional step for differentiating between RVs and EVs (1, 4, 7, 9, 11, 23), or samples were differentiated by different lengths of RV and EV amplification products ...early detection of most if not all RV ... See full document
5
Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
... infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose ... See full document
5
Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... cooling step at 40°C for 10 s. Interestingly, EtBr led to a better detection signal than the SYBR green classically used with the LightCycler under the reaction conditions used ...real-time PCR or ... See full document
7
Use of TaqMan Real Time Reverse Transcription PCR for Rapid Detection, Quantification, and Typing of Norovirus
... conventional reverse transcription (RT)- PCR, a method that requires confirmation by probe or se- quence analysis (3, ...designing one set of primers to detect all strains with equal ... See full document
8
Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... expensive detection equipment. One of the most attractive features of the RT-LAMP assay is that it seems to have great advantage in terms of the monitoring of the amplification that can be accomplished by ... See full document
7
Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences
... of PCR amplification in the laboratory were ...by PCR amplification in a final volume of 20 l with 100 ng of PCR products, 5 pmol of primer, and 8 l of BigDye Terminators premix according to the ... See full document
6
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... real-time one-step RT-PCR ...Platinum one-step quantitative RT-PCR kit; Invitrogen, Paisley, Scotland), 900 nM each primer (H5Forward and H5Reverse), 200 nM TaqMan minor ... See full document
9
Rapid Detection of Respiratory Tract Viral Infections and Coinfections in Patients with Influenza Like Illnesses by Use of Reverse Transcription PCR DNA Microarray Systems
... PIV detection appeared significantly higher than those previously described in Peru (PIV prevalence of ...real-time PCR systems ...time PCR techniques reported viral coinfection rates in the 5 to 10% ... See full document
7
Kinetics of Poliovirus Shedding following Oral Vaccination as Measured by Quantitative Reverse Transcription PCR versus Culture
... efforts, detection of oral polio vaccine (OPV) virus in stool samples can provide information about rates of mucosal immunity and allow estimation of the poliovirus ...multiplex one-step ... See full document
6
Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... The use of SynTura technology (41) to develop assay calibrators had several distinct advantages over the use of plasmids or RNA transcripts containing the HEV target sequence. Unlike a DNA-containing plasmid or RNA ... See full document
10
Rapid Simultaneous Detection of Enterovirus and Parechovirus RNAs in Clinical Samples by One Step Real Time Reverse Transcription PCR Assay
... Before transcription each plasmid was purified, linearized at the 3 ⬘ end using the ap- propriate restriction enzyme, and cleaned by phenol-chloroform extraction fol- lowed by ethanol ...RNA transcription ... See full document
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