[PDF] Top 20 Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
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Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... simple, rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution ... See full document
6
Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... sequence analysis of Irish NoV isolates [12,13] and with that of other NoV circulating globally ...a rapid, sensitive and broadly reactive method for detecting and genotyping Human ...initial ... See full document
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Rapid detection of Echinococcus species by a high resolution melting (HRM) approach
... gel-free detection method for species belonging to the Echinococcus genus (Figure 2A and ...HRM analysis was reported for genotyping ... See full document
5
Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... and detection of various Phytophthoras in different plant species, tissues, and environmental samples (Schena et ...named high resolution melting (HRM) analysis has been employed, apart ... See full document
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Rapid Detection of Isoniazid, Rifampin, and Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates Using High Resolution Melting Analysis
... A high-resolution melting analysis (HRMA) assay was developed to detect isoniazid, rifampin, and ofloxacin resistance in Mycobacterium tuberculosis by targeting resistance-associated mutations ... See full document
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Molecular detection of rifampin, isoniazid, and ofloxacin resistance in Iranian isolates of <em>Mycobacterium tuberculosis</em> by high-resolution melting analysis
... The development of rapid molecular diagnostic methods in recent years has a significant impact on the early detection of resistance to major anti-TB drugs in MTB isolates, which helps in employing ... See full document
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Rapid Detection of Common HIV 1 Drug Resistance Mutations by Use of High Resolution Melting Analysis and Unlabeled Probes
... efficacy. High- resolution melting analysis (HRMA) has been used to successfully identify single nucleo- tide polymorphisms (SNPs) and to genotype viral and bacterial ...a rapid, inex- ... See full document
12
High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... tested. High-resolution melting (HRM) curve analysis has been widely used for many ...the detection of PZA resistance in Mycobacterium tuberculosis clinical ...960 analysis ... See full document
18
Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis
... a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacte- ... See full document
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Development of a high resolution melting analysis assay for rapid identification of JAK2 V617F missense mutation and its validation
... the method should be carefully considered as with using too sensitive methods the rate of false positive results increases, and with using a low sensitivity method there would be increased false negative ... See full document
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Development and Validation of a Comparative Genomic Fingerprinting Method for High Resolution Genotyping of Campylobacter jejuni
... QIAxcel high-throughput cap- illary electrophoresis system on DNA screening cartridges (Qiagen, Mis- sissauga, Canada) using the AM320 separation method and a 20-s injec- tion ... See full document
10
Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth
... the detection of tens or hundreds individual markers contributing to complex ...needed. High-resolution melting (HRM) analysis is a new method in which polymerase chain reaction ... See full document
8
High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... HRM assays were performed using a Rotor-Gene Q6000 Real-Time PCR system (Qiagen, Sydney, Australia). Each reaction was performed in a total volume of 20 μl con- taining 10 μl of Forget-Me-Not ™ qPCR Master Mix (Bio- ... See full document
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Rapid Detection and Identification of Aspergillus from Lower Respiratory Tract Specimens by Use of a Combined Probe–High Resolution Melting Analysis
... microbiology laboratories. In addition, as the assay is restricted to the analysis of a single target in a single tube, its costs are low. The EORTC/MSG has not yet included PCR-based techniques among its ... See full document
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DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF TIROFIBAN IN SERUM
... Recovery Studies in Spiked Human and Rat Serum Samples: The reason that serum samples were used for this study and collected from the normal human volunteers and from the normal rat before introducing the experimental ... See full document
6
Rapid Detection and Statistical Differentiation of KPC Gene Variants in Gram Negative Pathogens by Use of High Resolution Melting and ScreenClust Analyses
... ScreenClust HRM analysis. In order to standardize the interpretation process, the HRM results from each PCR run were analyzed with the ScreenClust HRM software (Qiagen, Hilden, Germany). First, HRM curves were ... See full document
5
High Resolution Melting Curve Analysis for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Clinical Isolates
... evaluated high-resolution melting (HRM) curve analysis as a tool for detecting rifampin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis in an accurate, affordable, and ... See full document
6
Assay development and high-throughput screening for small molecule inhibitors of a <em>Vibrio cholerae</em> stress response pathway
... For a screen to be considered “high-throughput,” it should be optimized for the smaller-scale formats used for such assays, typically using 384- or 1,536-well microtiter plates. Since we consistently ... See full document
9
Development and validation of stability-indicating high performance liquid chromatographic method for the estimation of everolimus in tablets
... shape, resolution and peak parameter (tailing factor, theoretical ...sample analysis and the method was more cost effective as it utilizes very less quantity of mobile ...HPLC method was ... See full document
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The rs8099917 Polymorphism, When Determined by a Suitable Genotyping Method, Is a Better Predictor for Response to Pegylated Alpha Interferon/Ribavirin Therapy in Japanese Patients than Other Single Nucleotide Polymorphisms Associated with Interleukin 28B
... Study population. Samples were obtained from 708 Japanese chronic hepatitis C patients and divided into groups of 292 patients (145 males and 147 females; mean age, 57.2 years) and 416 patients (194 males and 222 ... See full document
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