[PDF] Top 20 Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... new assay failed to detect acanthamoebae in 5 clini- cal specimens deemed positive by the Riviere assay, and because 3 of these organisms could not be identi- fied to the species level, we are unable ... See full document
8
Development and Validation of a Real Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples
... TaqMan-based real-time PCR assay targeting the internal transcribed spacer 2 (ITS2) region of the ribosomal ...The assay was highly specific, repro- ducible, and sensitive, with the ... See full document
7
Development and Validation of a Rotor Gene Real Time PCR Assay for Detection, Identification, and Quantification of Chlamydia trachomatis in a Single Reaction
... qualitative detection of C. tracho- matis DNA. Due to the detection and confirmation of ...this assay is ideally suited for labo- ratories with high ...Amplicor PCR for 1,000 consecutive pa- ... See full document
8
Development and Validation of a One Step Real Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses
... These clinical samples were obtained from a wide range of avian species and geographical areas during field and labora- tory ...The assay has been used for monitoring the AI virus in poultry and wild birds, ... See full document
5
Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... the clinical validation, the LightCycler assay failed to detect four specimens ...TaqMan assay, and the copy number proved to be very low ( ⬍ 3 copies/ ...TaqMan assay has the advan- ... See full document
8
Development and validation of a duplex real time PCR assay for the diagnosis of equine piroplasmosis
... multiplex assay with performance characteristics similar to those of well- established single-target qPCR assays recognized as sensitive for the detection of ...this assay was confirmed to be high ... See full document
12
Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... for PCR-based diag- nostic techniques [22], an IAC was added to each real- time PCR reaction and used as an indicator of potential PCR reaction ...the validation limit for IAC ... See full document
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Development and Validation of a Highly Accurate Quantitative Real Time PCR Assay for Diagnosis of Bacterial Vaginosis
... METHODS Clinical specimens. A longitudinal clinical study was performed at the vaginitis clinic of Wayne State University Medical School in Detroit, MI, ...4 clinical signs needed to be present for ... See full document
9
A New Real Time PCR Assay for Improved Detection of the Parasite Babesia microti
... standard assay for diagnosis of active babesiosis has been microscopic examination of Giemsa-stained thick and thin blood smears, an assay that requires specially trained personnel for ...of ... See full document
6
Validation of a pan orthopox real time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
... example, assay performance is called out several times in the 2015 FDA Animal Rule guidance: assays used to quantify the chal- lenge agent dose should be validated, and those used to detect biomarkers used as a ... See full document
9
Novel real-time PCR detection assay for Brucella suis
... on detection of antibodies speci fi c for smooth lipopolysac- ...years PCR-based methods became more and more important as fast and reliable methods to detect Brucella -speci fi c DNA from colony material or ... See full document
7
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Singleplex real-time PCR assays have been developed to detect ...the assay sensitivity for serogroups B, W135, and Y is ...improve assay sensitivity and develop multiplex assays to ... See full document
7
Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... the real-time PCR assay enabled de- tection of the tox gene in clinical specimens when standard PCR proved ...inadequate. Real-time PCR is sensitive, ... See full document
7
Development of a Real Time PCR Assay for Detection of Toxoplasma gondii in Pig and Mouse Tissues
... via real-time PCR. Assay specificity was confirmed against a panel of DNA samples from ...TaqMan assay was able to detect as little as ...of detection of from 100 ng to 100 fg of ... See full document
7
Development and Evaluation of a Real Time PCR Assay for Detection of Klebsiella pneumoniae Carbapenemase Genes
... TaqMan real-time PCR assay amplified a 399-bp product from the bla KPC ...The assay was validated with reference strains obtained from the Centers for Disease Control and Prevention ... See full document
5
Clinical Validation of the HPV Risk Assay, a Novel Real Time PCR Assay for Detection of High Risk Human Papillomavirus DNA by Targeting the E7 Region
... HPV-Risk assay uses 5- l input of the sample DNA and runs on an ABI7500 Fast Real-Time PCR system (Applied Biosystems) or an equivalent real-time PCR system with a run ... See full document
7
Development and validation of Burkholderia pseudomallei-Specific real-time PCR assays for clinical, environmental or forensic detection applications
... Although assay parameters like ruggedness, robustness and selectivity are not typically examined when developing and validating molecular assays, we anticipate that our methods will provide a framework for future ... See full document
9
Novel Multitarget Real Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens
... of detection (LLOD) per PCR based on genomic equivalents for the ABI 7500 ...day, time point, and plate as random effects; dilution was treated as a fixed ... See full document
8
Real Time Reverse Transcription PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens
... the detection of immunoglobulin M-specific antibodies to mumps virus in acute-phase serum samples, the isolation of mumps virus in cell culture, or by detection of the RNA of the mumps virus by reverse ... See full document
7
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... the assay covers six log 10 copies of nucleic acid template, where the lower limit was set at the LOD C T of ...the assay, template quantitation from samples containing a large range of concentrations can ... See full document
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