[PDF] Top 20 Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
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Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... fully recombinant AAV plasmids (pTRUF2 and p2ITRLacZ), which persisted in less than 1% of cell ...the p5 promoter in pGFPCap caused an in- crease in the efficiency of rAAV site-specific ...in ... See full document
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Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors
... Adeno-associated virus (AAV) is a member of the depen- dent parvovirus family that requires coinfection with a helper virus, such as adenovirus or herpesvirus, to undergo a produc- tive ... See full document
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Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
... these recombinant junctions, the crossover point with the vector occurred at or slightly downstream of the P5 pro- moter at AAV nt 250 ...viral DNA in this group of independently isolated ... See full document
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Rescue and Autonomous Replication of Adeno-Associated Virus Type 2 Genomes Containing Rep-Binding Site Mutations in the Viral p5 Promoter
... The Rep proteins encoded by the adeno-associated virus type 2 (AAV) play a crucial role in the rescue, replication, and integration of the viral ...helper virus, little ... See full document
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Efficient Replication of Adeno-Associated Virus Type 2 Vectors: a cis-Acting Element outside of the Terminal Repeats and a Minimal Size
... tripartite, cis-acting element in MVM DNA that is functionally similar to the ele- ment that we have identified in AAV2 (6, 53, ...to sequence positions 4489 to 4695, which is 213 bp upstream of the ... See full document
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Efficient Generation and Amplification of High-Capacity Adeno-Associated Virus/Adenovirus Hybrid Vectors
... genes: rep, encod- ing the nonstructural proteins Rep78, Rep68, Rep52, and Rep40, and cap, encoding the structural proteins VP1, VP2, and ...all cis-acting elements necessary for genome replication, pack- ... See full document
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Analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene products.
... assay rep- lication of the vectors and to assess levels of wt AAV contam- ...of DNA were loaded in each lane [data not ...full-length Rep protein is synthesized by this ...viral DNA was ... See full document
9
Recombinant Adeno-Associated Viral Vectors Are Deficient in Provoking a DNA Damage Response
... Adeno-associated virus type 2 (AAV2) provokes a DNA damage response that mimics a stalled replication ...of DNA repair proteins into foci associated with AAV2 ...whether ... See full document
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Rep-Mediated Nicking of the Adeno-Associated Virus Origin Requires Two Biochemical Activities, DNA Helicase Activity and Transesterification
... AAV Rep nicking of the hairpinned TR appears to require three sequence recognition elements within the ...22-bp sequence required for stable binding of Rep78 and Rep68 (23). This Rep binding ... See full document
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Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration
... large Rep proteins, p68 and p78, function as master controllers of the adeno-associated virus type 2 (AAV2) life cycle, involved in transcriptional control, in latency, in rescue, and in viral ... See full document
10
Adeno-associated virus rep protein synthesis during productive infection.
... In a previous study 34 in human 293 cells coinfected with AAV and adenovirus, the two major Rep proteins Rep78 and Rep52 were detected by immunoblotting analysis earlier after infection [r] ... See full document
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Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep protein.
... chromosomal DNA replication initiates from sites dispersed throughout the genome with cell cycle regula- ...a DNA-binding protein that is thought to function by nucleating polymerase (Pol) complexes for ... See full document
9
Adeno-Associated Virus (AAV) Rep Protein Enhances the Generation of a Recombinant Mini-Adenovirus (Ad) Utilizing an Ad/AAV Hybrid Virus
... quantifying virus particles by measuring the optical density at 260 ...hybrid virus was able to transduce GFP expression to nearly all of the cells in the culture at the same ...physical virus ... See full document
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Mechanism of Rep-Mediated Adeno-Associated Virus Origin Nicking
... 5. Rep endonuclease activity on RBE⬘ substitution ...ative Rep specific activity for each mutant was expressed as the fraction of mutant substrate nicked divided by the fraction of wt substrate nicked at ... See full document
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Interaction of wild-type and mutant adeno-associated virus (AAV) Rep proteins on AAV hairpin DNA.
... viral DNA replication has been reported for SV40 (16) and polyomavirus (38) by T an- tigens possessing mutations in their ATPase domains and in other regions of the SV40 T antigen (47, ...to DNA and block ... See full document
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Adeno-associated virus vector integration junctions.
... T4 DNA ligase, and DNA polymerases were from New England BioLabs, Beverly, ...conditions. DNA manipulation and Southern blot analysis were performed by standard procedures ...repetitive ... See full document
8
Adeno-associated virus Rep proteins target DNA sequences to a unique locus in the human genome.
... the DNA hybridization and FISH analyses. In six clones examined by DNA hybridization, a re- arrangement at AAVS1 was observed, suggesting that the lacZ plasmid had integrated at that ...the ... See full document
9
DNA-damaging agents greatly increase the transduction of nondividing cells by adeno-associated virus vectors.
... DISCUSSION We have demonstrated that the transduction efficiency of AAV vectors on nondividing cells can be increased by more than 2 orders of magnitude by treatment with DNA-damaging ag[r] ... See full document
6
Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.
... With the exception of P365T, mutants carrying mutations within this region of the Rep protein amino acids 326 to 419 are defective for both transactivation and replication in vivo and wi[r] ... See full document
8
Identification of Cellular Proteins That Interact with the Adeno-Associated Virus Rep Protein
... Ad DNA concatemers that would inhibit Ad infection and reduce the yield of Ad virus (95, ...double-stranded DNA inter- mediates during replication, it too has been shown to be a target of the MRN ... See full document
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