[PDF] Top 20 ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.

... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ... See full document

... DNA replication by 5- to 10-fold, whereas mutations at positions 3, 4, and 5 completely abolished it ...reduces binding by approximately 5-fold, whereas mutations at positions 3, 4, and 5 lower affinity by ... See full document

... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...DNA replication, T antigen must interact ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document

... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) shows that the ... See full document

... as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...protein, large T antigen in ... See full document

... sequence-specific binding to cellular DNA by LT plays a role in transformation, as LT mutants defective in binding to the SV40 origin of replication transform cells with the same efficiency as ... See full document

... inhibit T antigen in a func- tional assay. We generated a labeled, origin-containing, circu- lar, 388-bp DNA ...our binding reactions. The circular DNA was incubated under DNA ... See full document

... DNA replication, at the molecular level, have been provided by model systems, in particular, the small DNA tumor viruses including simian virus 40 (SV40) and papillomavirus (reviewed in ... See full document

... that simian virus 40 (SV40) large T antigen (LT) binds to the Bub1 kinase, a key regulator of the spindle checkpoint and chromosome ...Bub1 binding-dependent delay in the ... See full document

... their replication after infection of resting permissive cells or, alternatively, cel- lular transformation after infection of nonpermissive ...SV40 large T with p53 might provide a good example for ... See full document

... Simian virus 40 large T antigen is a multifunctional oncoprotein that is required for numerous viral functions and the induction of cellular ...transformation. T ... See full document

... We have also used DNA footprinting techniques to show indirectly that the assembly of T antigen leads to stepwise and cooperative binding to the early and late halves of the SV40 core or[r] ... See full document

... the T-anti- gen ...nucleotide binding domain, can also form both rings and filaments (81), suggesting that rings and filaments are alternate structures that form during binding to ...of T ... See full document

... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document

... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ... See full document

... The failure of the zinc finger mutant T antigens to form hexamers in response to ATP may account for defects in subsequent steps in the initiation of DNA replication; we propose that hex[r] ... See full document

... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document

... 2195-2207 0022-538X/92/042195-13$02.00/0 Copyright © 1992, American Society for Microbiology A Deletion in the Simian Virus 40 Large T Antigen Impairs Lytic Replication in Monkey Cells I[r] ... See full document

... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document