[PDF] Top 20 Epigenetic reprogramming and induced pluripotency

... idea, reprogramming of NSCs into iPS cells in the absence of exogenous Sox2 expression increases the overall efficiency by roughly fourfold (Eminli et ...to pluripotency and that additional rare events must ... See full document

... of pluripotency genes is delayed as donor cells become more differentiated For the experiments reported here, we have chosen to use nuclei from 10T1/2 cells and to compare these results with those of nuclei from ... See full document

... of pluripotency was in reality due to the viral expression of the transduced Oct4 and Sox2 genes, whereas these endog- enous genes were not reactivated, as demonstrated also by the high methylation state of their ... See full document

... treatment (Fig. 13, top left, purple). 1h increases in H3K27ac are strongest (Fig. 13, top left, pink vs. light green), and by 3h H3K27ac begins to return towards baseline levels. H3K27me3 enrichment does not show ... See full document

... after reprogramming of MEF-derived iPSCs and continues until a telomere length similar to that in ESCs is reached (Marion et ...low reprogramming efficiency and massive ...damage induced by the short ... See full document

... that epigenetic alterations underlie nickel ...of epigenetic alterations that occur post nickel-exposure, which potentially drives persistent changes to the tran- scriptome remains ... See full document

... the pluripotency tran- scriptional network in this basic metazoan species, including the specific functions of each of the key ...and pluripotency is coupled to these genes and also to Pln and ...that ... See full document

... OSKM- induced reprogramming to detect the expression profile of Sirt6 in this ...OSKM-induced reprogramming in MEFs ...core pluripotency genes including Oct4, Sox2, Nanog, and Esrrb ... See full document

... MyoD induced myotube formation in fibroblasts (Graf and Enver, ...in pluripotency regulation (reviewed in (Ralston and Rossant, ...cell reprogramming was revivified after the demonstration that ... See full document

... and reprogramming, epigenetic modifiers are thought to play a key role in ensuring the correct patterns of DNA methylation and histone modifi- cation for gene ...global epigenetic marks occurs ... See full document

... To test our hypothesis, we conducted RNA-seq to evaluate the influence of vitamin C treatment on the transcriptome of A2058 cells. In order to minimize po- tential secondary effects in the long term, A2058 cells were ... See full document

... of epigenetic regulation is already addressed ...of epigenetic changes on the gene lev- el, and, more importantly, study of cell type–specific alterations ...tion, induced neuroprotective proteins ... See full document

... Generally, it is well documented that the GV stage oocyte must undergo an intensive growth phase during which the cell accumulates all the necessary material that will be used subsequently during early embryo development ... See full document

... state of the pluripotent network. To address this gap in knowledge, we used single cell analysis and a range of functional assays to comprehensively characterise VPA_AFS cells and understand how they relate to hES cells. ... See full document

... whether reprogramming of eAge is separable from the developmental reprogram- ming resulting in iPS cells remained ...for epigenetic rejuvenation of HP1β mobility ...eAge reprogramming is indeed ... See full document

... Total cellular RNA was extracted using the TRIzol reagent (Ambion Life Technologies, Blijswijk, Netherlands). For reverse-transcription polymerase chain reaction (RT-PCR) analysis, cDNA was synthesized from total RNA (2 ... See full document

... in reprogramming terminally differentiated somatic cells and cancer cells into induced pluripotent cells and cancer cells with benign ...induce reprogramming from one cell type to another, including ... See full document

... use induced pluripotent stem cell (iPSC) reprogramming and the murine intestinal stem cell (ISC) system to model macroH2A’s overall impact on cell epigenetic identity from embryo to ...iPSC ... See full document

... Induced pluripotent stem cells (iPSCs) are generated from somatic cells by introduction of Yamanaka factors comprised of Oct4, Sox2, Klf4, and c-Myc (OSKM), and they are highly desired for applications in ... See full document

... of epigenetic repro- gramming in the developing germ cells of marsupials has never been ...of epigenetic repro- gramming between this distantly related mammal and the mouse, we analysed the methylation ... See full document