[PDF] Top 20 Evaluation of Three Rapid Diagnostic Methods for Direct Identification of Microorganisms in Positive Blood Cultures

... polymicrobial cultures accounted for only a small percentage of blood cultures in our study as well as in previously published studies, note that none of the methods evaluated were capable of ... See full document

... Automated blood culture systems that monitor blood culture bottles continuously for bacterial growth minimize the time necessary to detect positive blood ...in blood cultures, ... See full document

... toward rapid detection of pathogens from positive blood cultures involved peptide nucleic acid-based fluorescence in situ hybridization (PNA-FISH) ...amplification methods coupled with ... See full document

... and rapid method for the identification of Myco- bacterium species from isolated colonies ...conventional identification techniques, or a combination of both ...more-complete identification of ... See full document

... of positive blood culture bottles. For 279 blood cultures positive by the BACTEC 9240 blood culture system, Gram staining and routine cultures were ...All blood ... See full document

... for identification of microorganisms directly from sterile body fluids such as cerebrospinal fluid, in order to save the culturing time ...in-house methods enabled direct identification ... See full document

... vitro diagnostic Bruker’s MALDI Sepsityper kit (Sepsityper) has the potential for clinical routine use and standardization since the manufacturer is respon- sible for its regulatory approval and the quality of the ... See full document

... of microorganisms from liquid cultures of blood represents one of the most critical results generated by diag- nostic clinical microbiology laboratories in terms of therapeutic patient management and ... See full document

... centage identification to species level in monomicrobial cultures is variable across the studies from 56 to 100% and probably reflects the different methodologies and gold standards with which it was ... See full document

... SAB identification assays that are commercially available. These methods employ a variety of tech- nologies, including molecular amplification and/or detection, an- tigen detection, and culture-based ... See full document

... subcultured three times on the appropriate solid media before ...isolated microorganisms were always in the logarithmic growth phase, bottles were removed from the BacT/ALERT 3D microbial detection system ... See full document

... Rapid identification of microorganisms in blood cultures is required to optimize empirical treatment at an early ...to identification of microorganisms in ... See full document

... reliable identification of bacteria directly from blood cultures is important in clinical practice to guide appropriate antibiotic ...in direct identification of Staphylococcus aureus, ... See full document

... of direct inoculation of MicroScan dried overnight and of rapid panels with positive aerobic blood cultures obtained from the BacT/Alert to determine antimicrobial ...between ... See full document

... dilution series of antifungal agent was added and mixed. The modules were incubated at 35°C for 48 h, and the capacitance change (i.e., capacitance growth curve) in each well was continuously monitored with Bactometer. ... See full document

... Additionally, 28 isolates of Pseudomonas species and other oxidase-positive gram-negative bacilli obtained from clinical specimens were stained by IFA to test for possible cross-reaction[r] ... See full document

... on blood culture ...from positive blood ...addition, identification of 10 different genera was possible with profiles comprising two or three ...of three TRFs, the similarity of ... See full document

... Incidence densities of bacteremia workups with blood cultures positive for microorganisms other than coagulasenegative staphylococci workups with a positive culture per 1,000 days, by bi[r] ... See full document

... Even when radiometric methods are employed A total of 10 9.2% cultures were detected by to detect microorganisms in blood culture, only the AO method, and 14 12.8% were de- "blind" subcu[r] ... See full document

... A 4-h Micro-ID technique for direct identification of oxidase-negative gramnegative rods from positive blood cultures was compared to subculture and species identification of single colo[r] ... See full document