[PDF] Top 20 Evaluation of the Editing Process in Human Immunodeficiency Virus Type 1 Genotyping
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Evaluation of the Editing Process in Human Immunodeficiency Virus Type 1 Genotyping
... on editing strategies. A question- naire on editing practices was distributed to the 10 participat- ing laboratories ...in editing strategies among the ...to editing and trimmed sequences ... See full document
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Genotyping of TRIM5 locus in northern pig tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection
... Formation of TRIMCyp fusion gene in M. leonina. (A and B) The genomic sequences spanning from the 5' end of exon 8 to the 3' end of exon 8 (A) or to the 3'-UTR of CypA cDNA (B) were PCR amplified and subject to ... See full document
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Performance of the Affymetrix GeneChip HIV PRT 440 Platform for Antiretroviral Drug Resistance Genotyping of Human Immunodeficiency Virus Type 1 Clades and Viral Isolates with Length Polymorphisms
... In agreement with reports by other investigators (6, 7), we found little difference in the cDNA sequences obtained from the same plasma samples processed for either the Affymetrix assay or for conventional cycle ... See full document
5
Mapping and Characterization of the N-Terminal I Domain of Human Immunodeficiency Virus Type 1 Pr55Gag
... in 1 ml of hypotonic buffer (10 mM Tris-Cl [pH 8.0], 1 mM EDTA) with protease inhibitors and then centrifuged at 1,000 ⫻ g for 10 min to remove nuclei and unbroken ... See full document
12
Functional Central Polypurine Tract Provides Downstream Protection of the Human Immunodeficiency Virus Type 1 Genome from Editing by APOBEC3G and APOBEC3B
... wild-type virus (6, 7, 14), but the mechanism through which the replication of these mutants is impaired is not fully ...transcription process, thereby reducing its vulnerability to cellular ...DNA ... See full document
6
Evaluation of a Multiple Cycle, Recombinant Virus, Growth Competition Assay That Uses Flow Cytometry To Measure Replication Efficiency of Human Immunodeficiency Virus Type 1 in Cell Culture
... recombinant virus single-cycle parallel infection assay, varies dramatically (5 to 81% of the wild-type reference strain NL4-3) ...erence virus are cultured ... See full document
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Human Immunodeficiency Virus Type 1 (HIV-1) Vpr Functions as an Immediate-Early Protein during HIV-1 Infection
... Human immunodeficiency virus type 1 (HIV-1), the etiolog- ical agent of AIDS, infects and ultimately incapacitates critical cellular components of the immune ... See full document
9
Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag
... that the p6 protein is lost (1, 35). We suggest that this obser- vation is also in accordance with our avidity model; specifically, because the fully processed p6 protein is a monomer (34), its removal from the ... See full document
6
RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.
... of human immunode- ficiency virus type 1 (HIV-1) has recently been found to bind specifically to the HIV-1 C element in ... See full document
9
Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... an evaluation of the ability to detect mixtures of wild-type and mutant amino acids at RT codons 41, 184, and 215 showed that two laboratories that used sequencing by hybridization performed at least as ... See full document
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Double-Stranded RNA Adenosine Deaminases Enhance Expression of Human Immunodeficiency Virus Type 1 Proteins
... nonspecific editing, since overexpression ...an editing-deficient ADAR1 mutant does not show enhancing activity for HIV-1 p24 expression in the supernatant of transfected ... See full document
9
Genotyping of B and Non B Subtypes of Human Immunodeficiency Virus Type 1
... During the study period of 33 months, a genotypic resistance test was con- ducted on 208 plasma samples from 208 patients, all with an HIV-1 viral load of ⬎ 1,000 copies/ml, as determined by the branched chain DNA ... See full document
5
Development of a Western Blot Assay for Detection of Bovine Immunodeficiency-Like Virus Using Capsid and Transmembrane Envelope Proteins Expressed from Recombinant Baculovirus
... The results of testing a group of bovine sera by our Western blot with the recombinant BIV p26 as the test antigen were 100% in agreement with those obtained in the reference West- ern blot assay with whole virus ... See full document
5
Lentivirus-Specific Cytotoxic T-Lymphocyte Responses Are Rapidly Lost in Thymectomized Cats Infected with Feline Immunodeficiency Virus
... vaccinia virus vector containing full-length FIV-Maryland gag or FIV env ...of virus-specific ...and virus load (tem- poral or peak) reflect the different assay systems employed (functional versus ... See full document
6
HIV Diagnosis in Resource Constrained Setting: How Good Is the Current Algorithm?
... Notably, the band for reactivity to p24 viral protein has been reported to be the most frequently occurring band among all the WBi patterns regardless of cohorts or study settings [23] [32] [33]. One of the possible sug- ... See full document
7
Transmission of Simian Immunodeficiency Virus SIVcpz and the Evolution of Infection in the Presence and Absence of Concurrent Human Immunodeficiency Virus Type 1 Infection in Chimpanzees
... drawn 1 to 2 months prior to exposure and at 2-week intervals for the first 2 months and then monthly until 6 months postexposure, when sampling was reduced to once every 2 months for the 1-year follow-up ... See full document
11
Potent and Broad Neutralization of HIV-1 Subtype C by Plasma Antibodies Targeting a Quaternary Epitope Including Residues in the V2 Loop
... The targets of broadly cross-neutralizing (BCN) antibodies are of great interest in the HIV vaccine field. We have identified a subtype C HIV-1-superinfected individual, CAP256, with high-level BCN activity, and ... See full document
14
Effect of semen and seminal amyloid on vaginal transmission of simian immunodeficiency virus
... factors modulating the infectiousness of HIV-1 in genital fluids may play a key role in the efficacy of sexual trans- mission of HIV-1 [6-8]. Multiple studies reported that semen boosts HIV-1 ... See full document
9
HIV infection of non dividing cells: a divisive problem
... More recently we seek to identify additional cellular fac- tors involved in nuclear import of HIV-1 RTCs. Using chromatographic procedures and the nuclear import assay we have isolated a near-homogeneous fraction ... See full document
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... virus. Here, we used Illumina Miseq platform to sequence the HIV-1 clinical samples. We took advantage of Nextera library construction technology, which allows NGS a tiny amount input cDNA (1ng) as opposed ... See full document
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