[PDF] Top 20 Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
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Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
... an extraction-free, filter-based template which is impregnated with denaturants, chelating agents, and a free-radical ...then DNA is trapped on the ...the DNA template ready for PCR ... See full document
5
Evaluation of two DNA extraction methods for the PCR based detection of eukaryotic enteric pathogens in fecal samples
... routine PCR methods in our ...both extraction procedures, positive stools were serially diluted with PCR-negative stool ...anonymized stool specimens could be used for ... See full document
6
Interlaboratory Evaluation of Different Extraction and Real Time PCR Methods for Detection of Coxiella burnetii DNA in Serum
... QIAamp DNA mini kit consistently yielded better results than either the MagNA Pure LC system and the NucliSens EasyMag (both automated) or the High Pure viral nucleic acid kit ...different PCR assays, with ... See full document
5
Evaluation of the Novel Helicobacter pylori ClariRes Real Time PCR Assay for Detection and Clarithromycin Susceptibility Testing of H pylori in Stool Specimens from Symptomatic Children
... Successful detection of these muta- tions in cultured strains or gastric biopsy specimens has been described by the use of fluorescent in situ hybridization (26), PCR-restriction fragment length ... See full document
5
Evaluation of a New Commercial TaqMan PCR Assay for Direct Detection of the Clostridium difficile Toxin B Gene in Clinical Stool Specimens
... real-time PCR methods using anaerobic toxigenic cul- ture as the comparator have sensitivities ranging from ...real-time PCR using SYBR green chemistry on a highly conserved region of tcdB ... See full document
5
PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA
... Conventional methods for the detection and identification of Escherichia coli O157:H7 include growth of non-sorbitol- fermenting ...the detection of small numbers of ...multiplex PCR for the ... See full document
6
Evaluation of Two DNA Extraction Methods for Detection of Strongyloides stercoralis Infection
... real-time PCR, using two extraction protocols, with that of the Baermann ...Two stool samples from each partici- pant were processed by the Baermann method, and one subsample was processed by ... See full document
8
Blinded, Externally Controlled Multicenter Evaluation of Light Microscopy and PCR for Detection of Microsporidia in Stool Specimens
... of PCR protocols, especially by those who developed them, are generally satisfactory to ...of PCR protocols outside the field of virology, there have been rather contrasting ...the detection of ... See full document
5
Multicenter Comparison of Nucleic Acid Extraction Methods for Detection of Severe Acute Respiratory Syndrome Coronavirus RNA in Stool Specimens
... individual stool samples were pooled and a 10% suspension was generated; thus, a single sample matrix spiked with different concentrations of SARS CoV was used for all ...tained PCR amplification ... See full document
8
Evaluation of Culture Methods and a DNA Probe Based PCR Assay for Detection of Campylobacter Species in Clinical Specimens of Feces
... 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ... See full document
7
Real Time PCR Method for Detection of Encephalitozoon intestinalis from Stool Specimens
... laboratory methods used for the diagnosis of this disease. A real-time PCR assay was designed to assess DNA extraction methods and to detect three Encephalitozoon species in ...LC ... See full document
7
Highly Specific and Quick Detection of Mycobacterium avium subsp paratuberculosis in Feces and Gut Tissue of Cattle and Humans by Multiple Real Time PCR Assays
... real-time PCR amplicons III and IV, along with those of the previously described specific amplicons I and II, were demonstrated using 2 ...with DNA extracted by an RTP Spin Bacteria kit. Since stool ... See full document
10
Duplex Real Time SYBR Green PCR Assays for Detection of 17 Species of Food or Waterborne Pathogens in Stools
... using PCR to detect food- or waterborne pathogens in stool ...QIAamp DNA Stool Mini kit (Qiagen, Hilden, Ger- many) for extraction of PCR-quality DNA from liquid ... See full document
13
Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization
... the PCR while it was originally reported as positive for ...the PCR was performed, degradation of the spores and microsporidial DNA is possible, although the other positive samples were stored under ... See full document
5
Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... RT- PCR is to perform nested RT-PCR, which has been reported to be 10 to 1,000 times more sensitive than single-round RT- ...nested PCR is the high risk of carryover and cross-contamination (2, ... See full document
7
Optimized cultivation of Campylobacter concisus from gut mucosal biopsies in inflammatory bowel disease
... In this study, we tested the effect of cultivation in differ- ent atmospheres on recovery of emerging Campylobacter species from gut mucosal biopsies, collected from 52 adult patients with IBD and 26 healthy controls, ... See full document
6
Forest diseases : FTA cards and DNA sampling
... Appendix 5.3 Continued PCR amplification of the Acacia mangium root seedlings samples using ITS primers in different DNA extraction methods PCR Results 1 Samples.. DNA Extraction Method.[r] ... See full document
201
Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR IS6110: preliminary study
... molecular detection and strain differentiation of members of Mycobacterium tuberculosis complex have proved to be ...The DNA extraction method influences the amplification efficiency, causing ... See full document
6
Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions
... Detection of group A rotavirus in serial fecal specimens by RT-PCR: extraction and dilution compared with extraction and CF11 purification RT-PCR results Fecal specimen mo-day collected.[r] ... See full document
8
Purification of Enterocytozoon bieneusi Spores from Stool Specimens by Gradient and Cell Sorting Techniques
... Currently, only fecal specimens from persons infected with E. bieneusi are convenient sources for obtaining large quanti- ties of spores. The difficulty is the purification of the spores from the fecal material. ... See full document
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