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[PDF] Top 20 Exploring the Nuclear Envelope of Herpes Simplex Virus 1-Infected Cells by High-Resolution Microscopy

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Exploring the Nuclear Envelope of Herpes Simplex Virus 1-Infected Cells by High-Resolution Microscopy

Exploring the Nuclear Envelope of Herpes Simplex Virus 1-Infected Cells by High-Resolution Microscopy

... mock-infected cells grown in cell culture flasks were trypsinized and centrifuged at 150 ⫻ g for 8 ...until cells were ...sedimented cells, sandwiched between two flat aluminum specimen ... See full document

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Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Localization in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1

Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Localization in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1

... pair 1 (Table 1) and cloning the fragment into the EcoRI and SpeI sites of pBluescript KS(⫹) ...at high concentrations, the A206K mutation was used to prevent dimerization without significant ... See full document

14

Inhibition of the Host Translation Shutoff Response by Herpes Simplex Virus 1 Triggers Nuclear Envelope-Derived Autophagy

Inhibition of the Host Translation Shutoff Response by Herpes Simplex Virus 1 Triggers Nuclear Envelope-Derived Autophagy

... outer nuclear membranes as the main source of material for the formation of 4-membrane-layered au- tophagic ...the nuclear envelope but not macroautophagosomes during ...nofluorescence ... See full document

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Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells

Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells

... the cells were harvested and ICP4 protein complexes were isolated using the TAP pro- cedure as described in Materials and ...TAP-ICP4-infected nuclear extracts than from KOS-infected ... See full document

12

Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts

Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts

... HFS cells were infected with a lentivirus expressing either an anti-PML or an anti-luciferase control shRNA to create cell line HFS-shPML1 or HFS-shLuci, re- ...shPML1 cells (Fig. 7B), and ⬎ 95% of ... See full document

14

Characterization of VP22 in Herpes Simplex Virus-Infected Cells

Characterization of VP22 in Herpes Simplex Virus-Infected Cells

... the envelope (7). For herpes simplex virus (HSV) the tegument accounts for approximately 50% of the volume of the virion and more than 15 virus-encoded proteins have been reported to be ... See full document

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Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1

Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1

... were infected with HSV-1 at 0.1, 0.5, 1, 5, and 10 PFU/cell and analyzed for expression of a late HSV-1 protein, gD, by flow cytometry and confocal microscopy at time points ranging ... See full document

7

Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells

Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells

... a nuclear pore, enabling the entry of the viral genome into the nucleus and initiation of viral gene expression ...inner nuclear membrane (INM) and outer nuclear membrane (ONM) by a unique ... See full document

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Multiple Roles of the Cytoplasmic Domain of Herpes Simplex Virus 1 Envelope Glycoprotein D in Infected Cells

Multiple Roles of the Cytoplasmic Domain of Herpes Simplex Virus 1 Envelope Glycoprotein D in Infected Cells

... erpes simplex virus 1 (HSV-1) is classified in the Alphaherpes- virinae subfamily of the Herpesviridae family and is one of the best-studied members in the family ...enveloped virus ... See full document

12

Nuclear processing of viral high-molecular-weight RNA in cells infected with herpes simplex virus type 1.

Nuclear processing of viral high-molecular-weight RNA in cells infected with herpes simplex virus type 1.

... At the end of the labeling period, the cells were rinsed three times with ice-cold Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Nuclear RNA isolated from cells infec[r] ... See full document

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The herpes simplex virus 1 UL11 proteins are associated with cytoplasmic and nuclear membranes and with nuclear bodies of infected cells.

The herpes simplex virus 1 UL11 proteins are associated with cytoplasmic and nuclear membranes and with nuclear bodies of infected cells.

... electron microscopy, the antigenicity of the pro- teins is preserved at a cost of ultrastructural detail inasmuch as osmium fixatives and lead stains are not ...electron microscopy protocols were also ... See full document

9

Nuclear Pore Composition and Gating in Herpes Simplex Virus-Infected Cells

Nuclear Pore Composition and Gating in Herpes Simplex Virus-Infected Cells

... in infected cells Fig. 4b, lanes 1 and ...lanes 1 and 2) and in the recruitment of a population of Nup358 into MAb414- precipitable complexes ...the nuclear envelope during ... See full document

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Sialic Acid on Herpes Simplex Virus Type 1 Envelope Glycoproteins Is Required for Efficient Infection of Cells

Sialic Acid on Herpes Simplex Virus Type 1 Envelope Glycoproteins Is Required for Efficient Infection of Cells

... viral envelope glycopro- teins was important for maintaining infectivity, gradient-puri- fied virions were digested with ...either 1 h or 4 h, and the titer of infectious virus was determined by ... See full document

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Accumulation of herpes simplex virus type 1 RNAs of different kinetic classes in the cytoplasm of infected cells.

Accumulation of herpes simplex virus type 1 RNAs of different kinetic classes in the cytoplasm of infected cells.

... 144 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have analyzed the accumulation of herpes simplex virus type 1 RNA of the immediate early IE; infected cell polype[r] ... See full document

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Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.

Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.

... Because little self-complementary RNA was Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Nuclei isolated from herpes simplex virus HSV-infected KB cells actively synth[r] ... See full document

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RNA Synthesis in Cells Infected with Herpes Simplex Virus VII. Control of Transcription and of Transcript Abundancies of Unique and Common Sequences of Herpes Simplex Virus 1 and 2

RNA Synthesis in Cells Infected with Herpes Simplex Virus VII. Control of Transcription and of Transcript Abundancies of Unique and Common Sequences of Herpes Simplex Virus 1 and 2

... Control of Transcription and of Transcript Abundancies of Unique and Common Sequences of Herpes Simplex Virus 1 and 2 Received for publication 17 January 1973 Analysis of the kinetics of[r] ... See full document

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Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells.

Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells.

... of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex DNA ...on herpes simplex ... See full document

5

Localization and putative function of the UL20 membrane protein in cells infected with herpes simplex virus 1.

Localization and putative function of the UL20 membrane protein in cells infected with herpes simplex virus 1.

... With the aid of this antiserum and an antibody to a constituent of the Golgi aparatus, we determined the following: i the UL20 gene is regulated as a -Yl gene, and the protein can be det[r] ... See full document

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Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.

Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.

... and 10% vol/vol tryptose phosphate broth Difco on 314 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sensitive mutants of herpes simplex virus type 1 repre[r] ... See full document

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Control of expression of the herpes simplex virus-induced deoxypyrimidine triphosphatase in cells infected with mutants of herpes simplex virus types 1 and 2 and intertypic recombinants.

Control of expression of the herpes simplex virus-induced deoxypyrimidine triphosphatase in cells infected with mutants of herpes simplex virus types 1 and 2 and intertypic recombinants.

... Of a series of temperature-sensitive mutants of HSV-2 strain HG52, ts9 and tsl3 failed to induce wild-type levels of the enzyme at nonpermissive temperature; ts9 was the most defective m[r] ... See full document

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