[PDF] Top 20 Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.
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Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.
... Analysis of SVT-Ag and p53 extracted from SF21 cells coinfected with both vEV55SVT and vEV55p53 reveals no major differences in the degree of phosphorylation of either of these proteins [r] ... See full document
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Formation of Covalently Modified Folding Intermediates of Simian Virus 40 Vp1 in Large T Antigen-Expressing Cells
... the simian virus 40 (SV40) major capsid protein Vp1, which take place in the infected cy- toplasm, have been shown to progress through disulfide-bonded Vp1 folding ...COS-7 cells that ... See full document
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Replication Stress and Mitotic Dysfunction in Cells Expressing Simian Virus 40 Large T Antigen
... Inducible expression of LT acutely elicits ␥ -H2AX foci indicative of replication stress prior to micronucleus ...BJ/tert cells stably expressing pInducer20 LT were examined by immunofluorescence with Dox ( ... See full document
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Wild-Type p53 Enhances Efficiency of Simian Virus 40 Large-T-Antigen-Induced Cellular Transformation
... BALB/c mouse embryo fibroblasts differing in p53 gene status (p53 ⴙ / ⴙ versus p53 ⴚ / ⴚ ) with simian virus 40 (SV40) revealed a quantitatively and qualitatively ... See full document
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Identification of the p53 protein domain involved in formation of the simian virus 40 large T-antigen-p53 protein complex.
... The N-terminal region deletion mutations amino acids 11 through 33 or carboxy-terminal localized linker insertion mutations amino acids 222 through 344 produced proteins that bound to th[r] ... See full document
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Induction of p53-Independent Apoptosis by Simian Virus 40 Small t Antigen
... for mouse polyomavirus st, this homologous region is not located at the immediate carboxy ...the cells of wild-type st to a predominantly nuclear pattern (compare ...in expression levels. ... See full document
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Complex of simian virus 40 large-T antigen and host 53,000-molecular-weight protein in monkey cells.
... Mock infections were performed identically except that dilutions of unin- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Mouse cells transformed by simian virus 40 SV4[r] ... See full document
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Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... ing cells remained ...small t helper function in SV40 transforma- tion and metabolic stabilization of p53 in complex with large ...of p53’s role in negatively controlling cell ... See full document
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Large-T-antigen-p53 complex formation is not cold sensitive in a cold-sensitive transformant induced by simian virus 40 mutant tsA1499.
... KATHLEEN RUNDELL Department of MicrobiologylImmunology and Cancer Center, Northwestern University Medical and Dental Schools, Chicago, Illinois 60611 Received 24 August 1983/Accepted 9 N[r] ... See full document
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Intracellular location and kinetics of complex formation between simian virus 40 T antigen and cellular protein p53.
... To test this fractionation scheme, SV40-transformed mouse 215 cells were labeled for 2 h with L-[35S]methionine and fractionated as described above, and the nuclear and cytoplasmic fract[r] ... See full document
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The ability of large T antigen to complex with p53 is necessary for the increased life span and partial transformation of human cells by simian virus 40.
... Although the formation of stable complexes is not required for virus replication 28, we show here that it is necessary for SV40 to induce transformed foci of human cells and to extend th[r] ... See full document
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The p53 complex from monkey cells modulates the biochemical activities of simian virus 40 large T antigen.
... i T+p53 has an enhanced ATPase specific activity 40; ii all subclasses of T that have altered ATPase activity also have altered amounts of bound p53 40; iii the coordinates of the p53 bi[r] ... See full document
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ATP-Dependent Simian Virus 40 T-Antigen–Hsc70 Complex Formation
... Simian virus 40 large T antigen is a multifunctional oncoprotein that is required for numerous viral functions and the induction of cellular ...transformation. T ... See full document
10
Inhibition of simian virus 40 T-antigen expression by cellular differentiation.
... Although none of the other clones differentiated as well as CSV3-1, repression of T-antigen expression was observed in all these cell clones as they underwent adipocyte differentiation, [r] ... See full document
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Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
... However, when chromatin was isolated from the variant 1103 19, which contains nine copies of the origin of DNA replication and lacks at least one and possibly two of the other high-affin[r] ... See full document
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Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.
... Moreover, SF9 T had ATPase activity comparable to that of T produced in monkey cells, exhibited helicase activity and SV40 origin-specific DNA binding, and was active in the SV40 DNA rep[r] ... See full document
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Simian virus 40 can overcome the antiproliferative effect of wild-type p53 in the absence of stable large T antigen-p53 binding.
... 8 Simian Virus 40 Can Overcome the Antiproliferative Effect of WildType p53 in the Absence of Stable Large T Antigen-p53 Binding DAN MICHAEL-MICHALOVITZ, FRUMA YEHIELY, EYAL GOTTLIEB, AN[r] ... See full document
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Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.
... 4799 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The phosphorylation pattern of simian virus 40 SV40 large tumor T antigen purified from insect cells infected with [r] ... See full document
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Stable T-p53 complexes are not required for replication of simian virus 40 in culture or for enhanced phosphorylation of T antigen and p53.
... To address this question, we compared the phosphorylation of p53 and T antigen in BSC-1 cells infected with wild-type SV40 with the phosphorylation obtained after infection with the resi[r] ... See full document
7
Measurements of the molecular size of the simian virus 40 large T antigen.
... This would be 218 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A measure of the molecular weight of the large simian virus 40 T antigen was sought by SDS-polyacrylam[r] ... See full document
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