[PDF] Top 20 Formation of a cruciform structure at the simian virus 40 replication origin abolishes T-antigen binding to the origin in vitro.
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Formation of a cruciform structure at the simian virus 40 replication origin abolishes T-antigen binding to the origin in vitro.
... 293-297 0022-538X/85/100293-05$02.00/0 Copyright © 1985, American Society for Microbiology NOTES Formation of a Cruciform Structure at the Simian Virus 40 Replication Origin Abolishes T-[r] ... See full document
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Topoisomerase I Associates Specifically with Simian Virus 40 Large-T-Antigen Double Hexamer–Origin Complexes
... T antigen is the only viral protein involved in SV40 DNA ...viral origin specifically, and the second is to separate the DNA strands at the origin and at replication ...Upon ... See full document
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The T-antigen-binding domain of the simian virus 40 core origin of replication.
... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... of simian virus 40 (SV40) large T antigen play a critical role in DNA ...DNA binding and ATPase and helicase activities but with a severely reduced ability to unwind ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...large T antigen in the case of SV40 ... See full document
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the T-Ag OBD, we screened for crystal formation be- tween the T-Ag OBD with a range of DNA templates contain- ing regions of ...the T-Ag OBD bound to the single-stranded region of an oligonu- ... See full document
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... DNA binding and the ability to cause structure distortion of origin DNA, suggesting that single- stranded DNA binding is also needed for structural ...from origin binding in- ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... open-ring structure (a spiral), and the authors suggest the open ring may provide a mechanism whereby the single-stranded nucleic acid may enter the central channel of the ring without disrupting the hexamer or ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD which are defective for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates (i.e., the core origin [0], site I [I], ... See full document
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... for T-ag assembly events, we analyzed binding to larger subfragments of the core ...hexamer formation and nucleotide cofactor-dependent double-hexamer assembly (see ...support T-ag assem- bly, ... See full document
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Activation of simian virus 40 transcription in vitro by T antigen.
... 4591 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Simian virus 40 is repressed when the viral early gene product large tumor antigen TAg binds to specific sites withi[r] ... See full document
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A functional simian virus 40 origin of replication is required for the generation of a super T antigen with a molecular weight of 100,000 in transformed mouse cells.
... 2 A Functional Simian Virus 40 Origin of Replication Is Required for the Generation of a Super T Antigen with a Molecular Weight of 100,000 in Transformed Mouse Cells Received 28 March 1[r] ... See full document
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Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.
... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ... See full document
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cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Binding of[r] ... See full document
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Inhibition of structural changes in the simian virus 40 core origin of replication by mutation of essential origin sequences.
... Simian virus 40 SV40 large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication.. Molecular cloning: a laboratory manual, 2nd ed.[r] ... See full document
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Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids.
... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document
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Biochemical analysis of mutants with changes in the origin-binding domain of simian virus 40 tumor antigen.
... These activities include origin-specific and nonspecific DNA binding, melting of the imperfect palindromic sequence, untwisting of the AT-rich region, unwinding of origincontaining DNA, [r] ... See full document
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document
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Phosphorylation of Simian Virus 40 T Antigen on Thr 124 Selectively Promotes Double-Hexamer Formation on Subfragments of the Viral Core Origin
... with T-ag, this core origin subfragment supported both hexamer and double hexamer formation (39, ...plus T-ag and the T124A mutant (lanes 11 and 12, ...either T-ag or the T124A mutant, ... See full document
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