[PDF] Top 20 Genetic and Functional Diversity of Human Immunodeficiency Virus Type 1 Subtype B Nef Primary Isolates

... possible Nef functions by gaining a better understanding of Nef’s role in ...of Nef in simian AIDS ...of Nef function in simian AIDS. Mutations introduced into SIV Nef based on studies of HIV ... See full document

... F. McCutchan, J. Louwagie, and P. Hegerich (Henry M. Jackson Foundation Laboratory, Walter Reed Army Institute of Research, Rockville, Md.); C. Lopez-Galindez, I. Olivares, and J. Dopazo (Centro Nacional de Biologı´a ... See full document

... of human immunodeficiency virus type 1 (HIV-1) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained from 24 of 25 HIV-1-seropositive patients ... See full document

... analysis. Primary sequence data were assembled and edited with Geneworks (Intelligenetics, Mountain View, ...Square genetic distance matrices were gen- erated by using EDNADIST; the Jukes-Cantor model of ... See full document

... 2. Functional activity of HIV-1 nef alleles in in vitro ...and b) or HeLa CIITA (lane c) cells were transduced with eGFP-expressing HIV-1 NL4-3 particles containing a disrupted ... See full document

... the subtype designations have been powerful molecular epidemi- ological markers for tracking the course of the global pandemic (5, 24, ...However, genetic analysis revealed that there were in fact two ... See full document

... the isolates most (94KE103, subtype D) and least (SF162, subtype B) sensitive to CC chemo- kines (Table ...these isolates replicated with similar efficiencies (Table ... See full document

... against primary HIV-1 isolates (14, 60). AN1-Env B gp120 contains 31 potential N-linked glycosyla- tion sites (sequons), many more than the median of 25 re- ported for primary ... See full document

... Human immunodeficiency virus type 1 (HIV-1) genetic diversity, due to its high evolutionary rate, has long been identified as a main cause of problems in the ... See full document

... found subtype A isolates not only in Uganda and Zaire but also in neighboring Kenya and Djibouti, 1,000 miles to the ...northeast. Subtype C isolates, previously recovered from Zambia, Malawi, ... See full document

... that subtype C viruses might have envelope glycoproteins that are atypical in structure or function compared to viruses from other HIV-1 ...where subtype C viruses predomi- ...the subtype C ... See full document

... Human immunodeficiency virus type 2 (HIV-2) is much less pathogenic than HIV-1, and HIV-2 infection is associated with plasma viral loads significantly lower than those found in ... See full document

... No depletion of CD4+ human thymocytes was observed in implants infected with either HIV-lJRCSF or its nef mutant at 3 or 6 weeks postinfection compared with controls consisting of mock-i[r] ... See full document

... and B and ...of 1 and standard deviation of 8 (we increased the latter relative to another study [47] because the larger number of epitopes pro- duced an averaging effect on ...blocking Nef ... See full document

... within Nef sequences from a single patient at a single time point to be of interest, (ii) we consider variation in Nef sequences from a single patient at different time points to be potentially significant, ... See full document

... is Nef not required for HIV-1 replication in many immortalized T-cell lines (41, 57)? Assuming that CD4 down- regulation by Nef is necessary for viral egress and/or optimal virion infectivity, a ... See full document

... provide genetic evidence that MA is dispensable for the ability of Nef to enhance the infec- tivity of HIV-1 virions produced either in CD4-negative ad- herent cells or in a T-cell ...that Nef ... See full document

... Does Nef act directly or indirectly? The infectivity of a virus expressing a nonmyristoylated form of the protein is as im- paired as that of a nef-deleted ...virus. Nef must therefore ... See full document

... To evaluate the effect of wild-type and cysteine mutant ELI and LAI Nef proteins on virus replication, the CD4+ T-cell line Jurkat and primary peripheral blood mononuclear cells PBMCs we[r] ... See full document

... and genetic bottleneck imposed by ARV ...of genetic diversity and divergence was observed after 2 years of ARV ...HIV-1 genetic diversity in both ... See full document