[PDF] Top 20 Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.
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Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.
... to DNA encapsidated by wild-type ...of DNA-containing capsids from the nucleus and are consistent with the observation that no C capsids were found in the cytoplasm of AN-1-infected cells on ... See full document
11
Role of the Nuclease Activities Encoded by Herpes Simplex Virus 1 UL12 in Viral Replication and Neurovirulence
... in viral replication in cell cultures required characterization of a still-unidentified function(s) of UL12 that is independent of its nuclease ...its nuclease activities, since no ... See full document
6
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... for efficient envelopment at the inner nuclear membrane have been ...the DNA packaging process. In wild-type virus-infected cells, empty capsids are only rarely enveloped (35), and a variety ... See full document
13
Nucleolin Is Required for an Efficient Herpes Simplex Virus Type 1 Infection
... regulation, viral replication, and cellular stress re- sponses (3, ...like viral DNA replication, virus assembly, and control of intracellular trafficking (for reviews see ... See full document
12
The Pre-NH2-Terminal Domain of the Herpes Simplex Virus 1 DNA Polymerase Catalytic Subunit Is Required for Efficient Viral Replication
... the replication fork or other- wise positively impacts viral DNA ...the viral helicase-primase complex, has been shown to interact with HSV-1 Pol in vitro ...the replication fork ... See full document
9
Replication of Herpes Simplex Virus Type 1 within Trigeminal Ganglia Is Required for High Frequency but Not High Viral Genome Copy Number Latency
... is required for the efficient establishment of latency is consistent with several other ...HSV DNA present in mice latently infected with the TK-negative mutant ...here. Viral ... See full document
11
Elimination of Mitochondrial DNA Is Not Required for Herpes Simplex Virus 1 Replication
... with herpes simplex virus type 1 (HSV-1) results in the rapid elimination of mitochondrial DNA (mtDNA) from host ...the viral alkaline nuclease (UL12) ... See full document
10
Herpes Simplex Virus 1 ICP22 but Not US1.5 Is Required for Efficient Acute Replication in Mice and VICE Domain Formation
... with 1 g of KOS viral DNA and ...of DNA) according to the manufacturer’s ...of viral DNA and Southern blot analyses ...mutant viral DNA with the wild-type ... See full document
10
Pocket Protein p130/Rb2 Is Required for Efficient Herpes Simplex Virus Type 1 Gene Expression and Viral Replication
... As viral DNA synthesis pro- ceeds, the staining pattern evolves to a globular appearance as replication compartments begin to fill the nucleus (15, 48, ...of viral DNA ... See full document
12
Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication.
... that efficient translocation of the helicase-primase complex into the nucleus required coexpres- sion of all of its subunits ...in replication compartments. Within the large globular ... See full document
8
Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... that viral DNA in HSV-1-infected cells that is in viral replication forms is sensitive to SN digestion, whereas unit-length and encapsidated viral DNA is resistant to SN ... See full document
12
Processing of Lagging-Strand Intermediates In Vitro by Herpes Simplex Virus Type 1 DNA Polymerase
... active DNA replication were identified by the incorporation of 5⬘ bromo-2-deoxyuridine ...antibody. Viral DNA replication compart- ments in nontransfected or transfected cells were ... See full document
14
In Vitro Processing of Herpes Simplex Virus Type 1 DNA Replication Intermediates by the Viral Alkaline Nuclease, UL12
... that alkaline nuclease does not completely degrade well ...well DNA is the first published DNA substrate that is not digested to completion by this ...well DNA must contain a structural ... See full document
10
The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... in herpes simplex virus type 1 (HSV-1)-infected cells: A (empty), B (in- termediate), and C (full) (15, ...either DNA or ...38). Replication of viral ... See full document
11
ICP0 Is Required for Efficient Reactivation of Herpes Simplex Virus Type 1 from Neuronal Latency
... infectious virus in cells or tissues that contain viral genomes, whereas reactivation has been defined as the de novo production of new infectious virus from cells containing latent viral ... See full document
10
DNA Mismatch Repair Proteins Are Required for Efficient Herpes Simplex Virus 1 Replication
... in virus yield (10- and 20-fold, respec- tively); however, analysis of the block in the virus life cycle revealed that MLH1 acts at a step prior to that of ...is required in normal human cells but ... See full document
13
Evidence that the herpes simplex virus type 1 uracil DNA glycosylase is required for efficient viral replication and latency in the murine nervous system.
... Assessment of the latent viral DNA burden by quantitative PCR QPCR demonstrated a reduction in the number of copies of the Ung- mutant viral Downloaded from http://jvi.asm.org/ on Novemb[r] ... See full document
10
A Mutation in the DNA Polymerase Accessory Factor of Herpes Simplex Virus 1 Restores Viral DNA Replication in the Presence of Raltegravir
... all viral stocks were maintained as mother pools that were used to seed working ...The herpes simplex virus 1 F strain [HSV-1(F)], referred to as the wild type in this ... See full document
9
The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.
... replicated viral DNA prior to its packaging into ...of viral polypeptides which play a role in this encapsidation process have been identified (reviewed in refer- ence 23), the actual protein which ... See full document
9
Topoisomerase II cleavage of herpes simplex virus type 1 DNA in vivo is replication dependent.
... Figure 8 summarizes the cleavages mapped in vivo in this DISCUSSION Topoisomerase II consensus sequences are distributed throughout the HSV-1 genome, and some of these sites are recogniz[r] ... See full document
8
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