[PDF] Top 20 Human Immunodeficiency Virus Type 1 Replication Is Modulated by Host Cyclophilin A Expression Levels

... in virus replication in Jurkat cells and H9 cells are explained by the higher levels of cyclo- philin A expressed in H9 cells ...of cyclophilin A packaged into the virions that these cells ... See full document

... noted. Levels of viral products were significantly increased when CAML expression was reduced ...surface expression of Tetherin and was not sufficient to generate an HIV-1-restric- tive ... See full document

... inducible expression of multimers of PR could be optimized to result in complete cleavage of precursor molecules, as well as cytotoxic ...for expression of PR from this ...constitutive levels of PR. ... See full document

... detectable levels of CCR5 on NHOKs, and HIV-1 virions establish re- verse-transcribed DNA structures in these ...productive expression of the HIV genome was minimal for CCR5-tropic ...HIV-1 ... See full document

... the cyclophilin-CA ...HIV-1 replication in different cell types might be due to variations in CypA expres- sion levels (2, ...CypA expression levels in the cell lines used in ... See full document

... p24 levels were ...determine levels of expression in the transfected cells relative to endog- enous expression in primary human cells that normally express Cav-1 (primary ... See full document

... leukemia virus requires cell division to establish a productive infection (24, 32, ...leukemia virus requires the dissociation of nuclear membranes during mitosis, which in theory leads to preintegration ... See full document

... RNA levels in coinfected children ...inhibited replication of both CCR5- and CXCR4-tropic strains of HIV-1 in PBMCs cultured in ...HIV-1 replication, although not to the same degree as ... See full document

... To understand what advantage the dependence on CyPA provides for HIV-1 will most likely require elucidation of the function of CyPA in HIV-1 replication. Over the course of the retrovirus life cycle, ... See full document

... HIV-1 replication, depending on the capsid and the host ...suppress replication of HIV-1 in HeLa cells (1, 45, 46), these agents exert minimal effects on the early phase of ... See full document

... of type 1 IFN genes (IFNA and IFNB), and therefore, any IFN synthesis in these cells is encoded by the transduced IFN gene ...and levels of biologically active IFNA were measured as described in ... See full document

... gene expression, but only macrophages and microglia support sig- nificant expression of late, fully processed HIV-1 gene prod- ...HIV-1 replication cycle have been described previously ... See full document

... mRNA levels in infected MDDCs but not to interfere with cell proliferative activity (data not ...the expression of HIV-1 recep- ...of expression of different cell surface receptors, including ... See full document

... HIV replication occurs down- stream of viral entry and proviral gene ...HIV replication and resulted in ⬃ 90% degradation of Rab9 mRNA and an ⬃ 75% decrease in ...HIV-1 replication with Rab9 ... See full document

... Drug resistance assays. Drug resistance assays were performed as previously described (24). Briefly, HIV-1-infected MT-2 cells were incubated in a 96-well flat-bottom plate in the presence or absence of various ... See full document

... enzyme cyclophilin A in TRIM5 ␣ antiviral activity. Cyclophilin A is recruited into nascent human immunodeficiency virus type 1 (HIV-1) virions as well as incoming ... See full document

... of host-virus interactions at an “organism” level, since both virus and host could be genetically ...while expression of CD4, a coreceptor, and a functional CycT1 pro- tein would be ... See full document

... another host compo- nent that traffics the PIC through the nuclear ...gene expression and neural crest cell migration ...other virus may deserve further ... See full document

... discovery of the owl monkey fusion protein TrimCyp (48), the existence of which hinted that the functions of CypA and TRIM5 were somehow associated. In cells from rhesus ma- caques and African green monkeys, the TRIM5 ␣ ... See full document

... to human CyPA has led to the suggestion that CyPA may play a role in virion ...HIV-1 replication defective by allowing highly stable CA-CA interactions, thereby interfering with virion ...higher ... See full document