[PDF] Top 20 Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs.
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Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs.
... ARRIGO`* Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425-2230'; Howard Hughes Medical Institute, School of Medicine, Uni[r] ... See full document
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RNA aptamers selected to bind human immunodeficiency virus type 1 Rev in vitro are Rev responsive in vivo.
... selected RNAs thus provided ideal tools to distinguish between two hypotheses: (i) that the primary sequence and secondary structure of the wild-type RBE are prerequisites for Rev responsiveness or ... See full document
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Exchange of the Basic Domain of Human Immunodeficiency Virus Type 1 Rev for a Polyarginine Stretch Expands the RNA Binding Specificity, and a Minimal Arginine Cluster Is Required for Optimal RRE RNA Binding Affinity, Nuclear Accumulation, andtrans-Activation
... RNA binding specificity of Rev. Using nine-arginine substituted Rev–MS-C mutants forced to express a truncated nine-arginine substituted Rev of 87 residues or a frame-shifted Rev–MS-C ... See full document
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The Carboxy-Terminal Region of the Human Immunodeficiency Virus Type 1 Protein Rev Has Multiple Roles in Mediating CRM1-Related Rev Functions
... of Rev. First, LMB reduces in vivo Rev-Rev interactions in a concentration-dependent manner in the mammalian two- hybrid assay ...of Rev-HA with zz-Rev, the specificity of ... See full document
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Human Immunodeficiency Virus Rev-Binding Protein Is Essential for Influenza A Virus Replication and Promotes Genome Trafficking in Late-Stage Infection
... A virus uses cellular protein transport systems ...Rab11- dependent recycling endosomes) for genome trafficking from the nucleus to the plasma membrane, where new virions are ...probably ... See full document
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Membrane binding of human immunodeficiency virus type 1 matrix protein in vivo supports a conformational myristyl switch mechanism.
... not required for stable membrane ...resistant binding of this protein with membranes ...membrane binding in in vitro binding assays ...myristylation-dependent binding of ... See full document
11
Diminished Rev-Mediated Stimulation of Human Immunodeficiency Virus Type 1 Protein Synthesis Is a Hallmark of Human Astrocytes
... for human immunodeficiency virus type 1 (HIV-1) in the central nervous system with attenuated virus replication in vivo and in ...of Rev-dependent ... See full document
11
Restriction of Human Immunodeficiency Virus Type 1 Rev Function in Murine A9 Cells Involves the Rev C-Terminal Domain
... the Rev-dependent expression of both intron-containing and intron-lacking transcripts was restricted in A9 cells, HeLa and A9 cells were transfected with a gag expression vector, pCMVGag2RRE, and evaluated ... See full document
7
Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface
... RNA binding and multimerization activities of nonfunctional Rev mutant proteins in a mammalian cell culture ...in vivo RRE SLIIB RNA binding and multimer formation by Rev (see the text ... See full document
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Rev Inhibition Strongly Affects Intracellular Distribution of Human Immunodeficiency Virus Type 1 RNAs
... the human immunodeficiency virus type 1 (HIV-1) RNA maturation pathways, we analyzed the intracellular distribution of HIV-1 RNA and the viral regulatory proteins ... See full document
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A Bipartite Membrane-Binding Signal in the Human Immunodeficiency Virus Type 1 Matrix Protein Is Required for the Proteolytic Processing of Gag Precursors in a Cell Type-Dependent Manner
... Protein analysis and sera. Immunoblotting of cell lysates or extracellular viral particles was performed as previously described (14). An HIV-1-positive human serum was obtained from an ... See full document
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Dominant negative mutants of human T-cell leukemia virus type I Rex and human immunodeficiency virus type 1 Rev fail to multimerize in vivo.
... To determine whether the previously described activation and multimerization domains of Rev were required for this in vivo protein-protein interaction, three previously described missens[r] ... See full document
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Conserved functional organization of the human immunodeficiency virus type 1 and visna virus Rev proteins.
... The hybrid protein bearing the basic domain of the visna virus Rev protein and the leucine domain of HIV-1 Rev, termed L/Rev, proved fully able to trans activate visna virus structural p[r] ... See full document
5
The I Domain Is Required for Efficient Plasma Membrane Binding of Human Immunodeficiency Virus Type 1 Pr55Gag
... Gag protein expression constructs fused to a human codon-optimized form of green fluores- cence protein (GFP) were used in this ...encephalomyocarditis virus for cap-indepen- dent translation ... See full document
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A novel human immunodeficiency virus type 1 protein, tev, shares sequences with tat, env, and rev proteins.
... To determine whether either p28tev or p22 bears this posttranslational modification, HeLa cells transfected with fB, mutant 107, or a mixture of pL3tat and pL3crev were labeled with 32Pi[r] ... See full document
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Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm.
... Indirect immunofluorescent double labeling of HeLa/crev cells showing Rev in the snRNP-rich nucleoplasmic speckles small arrows, in the nucleoli arrowheads, in a perinuclear zone, and in[r] ... See full document
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Different sites of interaction for Rev, Tev, and Rex proteins within the Rev-responsive element of human immunodeficiency virus type 1.
... Both human immunodeficiency virus HIV and human T-cell leukemia viruses HTLV regulate the expression of their structural proteins by specific viral factors called Rev and Rex, respective[r] ... See full document
8
Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides
... stable binding site for NC, we found that initial experiments with the tetranucleotide TGTG failed to give reproducible ...four binding sites for biotinylated ligands, the ratio enabled us to calculate the ... See full document
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Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.
... A RIP of 32P-labeled proteins from BIV127-infected BLAC-20 cells, using rabbit anti-Rev peptide serum lane 1 or normal rabbit serum lane 2.. B Immunoblot developed with rabbit anti-Rev p[r] ... See full document
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Persistence of attenuated rev genes in a human immunodeficiency virus type 1-infected asymptomatic individual.
... of Rev activation domain sequences in the HIV da- tabase, with regard to the leucines at positions 75, 78, 81, and 83, demonstrated only one divergent sequence (HIVMN) within the subtype B viruses (n 5 65) as well ... See full document
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