[PDF] Top 20 Human Immunodeficiency Virus Type 1 Nef Protein Targets CD4 to the Multivesicular Body Pathway
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Human Immunodeficiency Virus Type 1 Nef Protein Targets CD4 to the Multivesicular Body Pathway
... for CD4 downregulation. Since Nef binds to the tail of CD4, we thought that perhaps Nef itself became ubiquitinated, thus conferring on CD4 the ability to be sorted in a Ub-dependent ... See full document
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Nef-Mediated Downregulation of CD4 Enhances Human Immunodeficiency Virus Type 1 Replication in Primary T Lymphocytes
... of Nef have been demon- strated in culture, including downregulation of cell surface CD4 molecules, downregulation of cell surface major histo- compatibility complex (MHC) class I molecules, enhancement of ... See full document
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Nef-Induced CD4 Downregulation: a Diacidic Sequence in Human Immunodeficiency Virus Type 1 Nef Does Not Function as a Protein Sorting Motif through Direct Binding to β-COP
... of Nef functions in CD4 downregulation, the activity of the NefEE mutants was analyzed by cotransfection of HeLa cells stably expressing CD4 (HeLa-CD4) with the Nef expression vector ... See full document
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Induction of phosphorylation of human immunodeficiency virus type 1 Nef and enhancement of CD4 downregulation by phorbol myristate acetate.
... of Nef in regulating the stability, cellular localization, and function of the protein, extensive mutagenesis and functional analysis will be ...PMA-insensitive CD4 mutant by Nef after ... See full document
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Nef-Induced CD4 Endocytosis in Human Immunodeficiency Virus Type 1 Host Cells: Role of p56lck Kinase
... disrupted nef gene followed by an internal ribosomal entry sequence (IRES) and the enhanced green fluorescent protein (EGFP) (pBR-NL43-env*nef⫹vpu*-IRES-EGFP and pBR-NL43- ... See full document
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Detection of Human Immunodeficiency Virus Type 1 Nef and CD4 Physical Interaction in Living Human Cells by Using Bioluminescence Resonance Energy Transfer
... against human immunodeficiency virus type 1 (HIV-1) Nef and ...for 1 h with protein A/G plus agarose and centri- fuged, and the pellets were washed six times ... See full document
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Dynamic Evolution of the Human Immunodeficiency Virus Type 1 Pathogenic Factor, Nef
... This Nef was found to be active for PAK-2 activation ...(Fig. 1; see also ...sub- type B Nefs with R191 were then tested in preliminary ...downregulate CD4 and MHC class I ...of Nef ... See full document
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Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor
... constructs. Human CD4 cDNA was amplified by PCR and inserted into the EcoRI and SalI sites of ...the CD4-dileucine mutant (LL413,414AA) cDNA, generating ...HIV-1 Nef (NL4-3 variant) was ... See full document
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Pseudotyping human immunodeficiency virus type 1 (HIV-1) by the glycoprotein of vesicular stomatitis virus targets HIV-1 entry to an endocytic pathway and suppresses both the requirement for Nef and the sensitivity to cyclosporin A.
... of Nef action, HIV-1 ex- pression vectors were cotransfected with the VSV-G expres- sion vector pHCMV-G into human 293T ...in virus infectivity was observed (data not ...a CD4-negative ... See full document
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CD4 Glycoprotein Degradation Induced by Human Immunodeficiency Virus Type 1 Vpu Protein Requires the Function of Proteasomes and the Ubiquitin-Conjugating Pathway
... The human immunodeficiency virus type 1 (HIV-1) vpu gene encodes a type I anchored integral membrane phosphoprotein with two independent ...regulates virus release ... See full document
9
Functional Characterization of the Human Immunodeficiency Virus Type 1 Nef Acidic Domain
... not CD4 down- ...crucial Nef residue that has not been included in either the NPA or NMA model is ...downregulation, CD4 downregulation, and the enhancement of infectivity but activates PAK2 twofold ... See full document
12
Nef Enhances Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Matrix
... of Nef to enhance the infec- tivity of HIV-1 virions produced either in CD4-negative ad- herent cells or in a T-cell ...that Nef targets HIV-1 budding to lipid rafts in ... See full document
6
Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
... The Nef protein of human immunodeficiency virus type 1 (HIV-1) stimulates viral ...disrupted nef genes were 4 to 40 times less infectious than ... See full document
9
Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells.
... To test whether the expression of the consensus Nef protein also correlated with a decrease in surface CD4 expression, the transduced HuT-78 and AKR1-G1 cells were stained for human or m[r] ... See full document
9
A Basic Patch on α-Adaptin Is Required for Binding of Human Immunodeficiency Virus Type 1 Nef and Cooperative Assembly of a CD4-Nef-AP-2 Complex
... with 1 mM 3-amino-1,2,4-triazole (3AT; ⫺His ⫹ 3AT plates). Wild-type HIV-1 NL4-3 Nef (or the cytosolic tail of mouse tyrosinase) was expressed as a GAL4BD fusion protein from the ... See full document
13
Leucine-Specific, Functional Interactions between Human Immunodeficiency Virus Type 1 Nef and Adaptor Protein Complexes
... HIV-1 Nef with distinct motifs from cellular proteins and correlated protein interactions with biologic ...of Nef provided a structurally favorable context for the presentation of a variety of ... See full document
13
Human Immunodeficiency Virus Type 1 Nef-Induced CD4 Cell Surface Downregulation Is Inhibited by Ikarugamycin
... a virus to remove its receptor from the cell surface ...receptor, CD4, from the cell surface. The envelope protein of HIV has been shown to block transport of CD4 to the cell surface by ... See full document
5
Disulfide bond formation in the human immunodeficiency virus type 1 Nef protein.
... To evaluate the effect of wild-type and cysteine mutant ELI and LAI Nef proteins on virus replication, the CD4+ T-cell line Jurkat and primary peripheral blood mononuclear cells PBMCs we[r] ... See full document
5
Direct In Vitro Binding of Full-Length Human Immunodeficiency Virus Type 1 Nef Protein to CD4 Cytoplasmic Domain
... Human immunodeficiency virus type 1 (HIV-1) Nef is a protein containing roughly 200 amino acid ...membrane-associated protein that is produced at the ... See full document
5
Producer-cell modification of human immunodeficiency virus type 1: Nef is a virion protein.
... Type 1 human immunodeficiency viruses encoding mutated nef reading frames are 10- to 30-fold less infectious than are isogenic viruses in which the nef gene is ...causes ... See full document
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