[PDF] Top 20 Identification of Medically Important Yeasts Using PCR Based Detection of DNA Sequence Polymorphisms in the Internal Transcribed Spacer 2 Region of the rRNA Genes
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Identification of Medically Important Yeasts Using PCR Based Detection of DNA Sequence Polymorphisms in the Internal Transcribed Spacer 2 Region of the rRNA Genes
... labelled PCR products, two parts PCR product and one part of GS-500 ROX size standard (Perkin-Elmer Applied Biosystems, Warrington, England) were added to deionized formamide (AMRESCO, Solon, Ohio), ... See full document
9
Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions
... by yeasts have increased in previous decades due primarily to the increasing population of immunocompromised ...of sequence analysis of the rRNA gene internal transcribed spacer ... See full document
8
Assessment of Ribosomal Large Subunit D1 D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
... the identification of fungal pathogens reflects significant improve- ments in DNA analysis in recent years, including the develop- ment of broad-range (panfungal) primers, kit-based automated sample ... See full document
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Genetic Diversity of Pneumocystis carinii f sp hominis Based on Variations in Nucleotide Sequences of Internal Transcribed Spacers of rRNA Genes
... of genes have been used to type Pneumocystis ...nucleotide sequence variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were used ... See full document
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Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S 23S rRNA Gene Internal Transcribed Spacer PCR Restriction Analysis
... for detection of MCC species and differentiation of MCC species from other species of rapidly growing mycobacteria (RGM) and from one another is ...method, based on the variable 16S-23S rRNA gene ... See full document
7
Rapid Molecular Identification of Pathogenic Yeasts by Pyrosequencing Analysis of 35 Nucleotides of Internal Transcribed Spacer 2
... Rapid identification of yeast species isolates from clinical samples is particularly important given their innately variable antifungal susceptibility ...the internal transcribed spacer ... See full document
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Identification of Medically RelevantTrichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database forTrichosporon Identification
... for PCR have been designed, based on the sequences of 18S or 26S rDNA (6, ...pathogenic yeasts. The relatively high levels of sequence similarity observed among some species appears to limit ... See full document
9
Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species
... Fungal rRNA genes are tandemly repeated, with each re- peat encoding 18S (small-subunit), ...unit) genes. Two other regions exist in each repeat: the internal transcribed spacer ... See full document
5
Rapid Identification of Candida dubliniensis Using a Species Specific Molecular Beacon
... sites. DNA sequence analysis of the internal transcribed spacer 2 (ITS2) region of rRNA genes from reference Candida strains was used to develop molecular ... See full document
8
Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts
... ITS1 sequence-based phylogenetic tree of 41 clinically significant yeast ...was based on 419 aligned positions of complete ITS1 sequences with adjacent partial sequences of 18S and ...ITS2 ... See full document
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Analysis of the 16S–23S rRNA Gene Internal Transcribed Spacer Region in Klebsiella Species
... nal transcribed spacer (ITS) sequence, which is not subject to the same selective pressure as the rRNA genes and conse- quently has a 10-times-greater evolution rate, appears to be able ... See full document
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Internal Transcribed Spacer Regions of rRNA Genes of Pneumocystis carinii from Monkeys
... 620-bp PCR prod- uct. The PCR products were cloned and ...18S rRNA genes of all 14 speci- mens were found to be identical, and only one base, the second base, of the first 14 bases of the 26S ... See full document
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... The internal transcribed spacer (ITS) regions, which are scattered among the mature rRNA genes are because they enable their own excision from the RNA transcript, have been sequenced ... See full document
7
Molecular confirmation of the occurrence in Germany of Anopheles daciae (Diptera, Culicidae)
... Despite having followed the recent literature and having denominated An. daciae a species, the authors do not consider the evidence given for the species status of An. daciae , separate from An. messeae , as convincing ... See full document
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Catheter associated bacteremia by Mycobacterium senegalense in Korea
... The M. fortuitum group included M. fortuitum, M. peregri- num, M. mucogenicum, M. senegalense, M. mageritense, and several newly described species such as M. septicum, M. houstonense, M. boenickei, M. neworleansense, and ... See full document
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Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue Specimens
... and DNA extractions were per- formed in a class II laminar flow ...cut using a sterile microtome blade and transferred to a 10-ml centrifuge tube (Sarstedt Australia, Technology Park, ...a 2-ml ... See full document
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PCR Analyses of tRNA Intergenic Spacer, 16S 23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter and Intraspecific Relationships ofEnterobacter cloacae Strains
... ingly important as the number and spectrum of nosocomial pathogens continue to expand ...an important nosocomial pathogen (9), and cases of infec- tion in surgical wards (29) and burn units (26) and of ... See full document
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... for 2 h. A 100-bp DNA ladder (Promega ...AGE, PCR products were analyzed with an ME instrument (model SV1100; Hitachi Electronics Engineering ...of PCR-amplified fragments by ... See full document
6
The role of zoonotic chlamydial agents in ruminants abortion
... kit, based on rPOMP-90-3, 4 and 3+4 antigens were designed by Bakhtiari et ...52% using Nested-PCR ...abortus based on ompA gene from tissue samples using real-time PCR ... See full document
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Update on Pneumocystis carinii f sp hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes
... carinii based on nucleotide sequence variations has been conducted by several investigators on several differ- ent loci (1, 5, 8–10, 12, 19, ...mitochondrial rRNA gene (12) and the ITS regions (7, ... See full document
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