[PDF] Top 20 Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions
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Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions
... SYA1) sequence typing scheme (42) and the fact that DNA sequence similarities were ⬍ 90% in the ITS1 se- quence, ...of yeast identification based on ITS1 sequences will ...related ... See full document
8
Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling Circle Amplification
... At present, ITS sequence analysis is the gold standard for dermatophyte identification. We confirm its accuracy in this capacity. However, this approach is expensive, has a turn- around time of 2 to ... See full document
8
Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... Bacterial strains. The type strains of 20 mycobacterial species from the Amer- ican Type Culture Collection and 1l8 M. tuberculosis and 87 NTM clinical isolates were used in this study. M. tuberculosis clinical ... See full document
7
Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2
... 99% sequence similarity with those of ...Arthrobotrys species, and ...14 species, including ...Arthrobotrys species have morphological features distinct from those of ...these species ... See full document
6
Identification of Aspergillus fumigatus and Related Species by Nested PCR Targeting Ribosomal DNA Internal Transcribed Spacer Regions
... Multiple sequence alignments and ...the sequence data and information from GenBank ...multiple sequence alignment results, we noticed that (i) the small-sub- unit, ...large-subunit regions ... See full document
6
Assessment of Ribosomal Large Subunit D1 D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
... Aspergillus species group identification still requires important improvement in the GenBank database, including submission of additional se- quence information for the ITS1 and ITS2 regions ... See full document
12
Identification of Medically Important Yeasts Using PCR Based Detection of DNA Sequence Polymorphisms in the Internal Transcribed Spacer 2 Region of the rRNA Genes
... of medically relevant yeasts can be time-consuming and inaccurate with current ...of sequence polymorphisms in the internal transcribed spacer 2 (ITS2) region of the rRNA genes as a ... See full document
9
Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts
... ITS1 sequence-based phylogenetic tree of 41 clinically significant yeast ...ITS2 sequence-based phylogenetic tree of 42 clinically significant yeast ...26S sequence-based phylogenetic ... See full document
10
Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... reported species of chlamydospore- and germ tube-positive yeast which has been isolated from the oral cavities of human immunodeficiency virus-infected ...two species have been determined by DNA ... See full document
6
Identification and Typing of Malassezia Species by Amplified Fragment Length Polymorphism and Sequence Analyses of the Internal Transcribed Spacer and Large Subunit Regions of Ribosomal DNA
... the identification and strain typing of Malassezia ...is important to iden- tify the different species correctly, as some studies have given preliminary indications that different Malassezia ... See full document
8
Generating and Testing Molecular Hypotheses in the Dermatophytes
... One important question regarding the epidemiology of dermatophytes is the issue of reactivation or ...lated species and strains. Appropriate species and strain typing in most organisms usually ... See full document
8
Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions
... of species or for performance of an epide- miological study, it is important to have a reliable method for the identification of dermatophyte ...(RFLP) analysis of mitochondrial DNA (mtDNA) ... See full document
5
Identification of Medically Important Candida and Non Candida Yeast Species by an Oligonucleotide Array
... of yeast infections has increased in the recent decades, with Candida albicans still being the most common cause of ...the internal transcribed spacer 1 (ITS 1) and ITS 2 sequences of the rRNA ... See full document
10
Systematic Internal Transcribed Spacer Sequence Analysis for Identification of Clinical Mold Isolates in Diagnostic Mycology: a 5 Year Study
... fungal species, which may show vari- ations in cell wall composition and in genomic ITS copies, hampers the implementation of a universal DNA extraction method (2, 22, ...ITS regions of some fungal isolates ... See full document
5
Identification of Medically RelevantTrichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database forTrichosporon Identification
... Identification of ubiquinone. The ubiquinone study was carried out only with the Trichosporon sp. strain M 9481 environmental isolate. The extraction of ubiquinone was performed following a procedure of Yamada and ... See full document
9
Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species
... other regions exist in each repeat: the internal transcribed spacer (ITS) region and the intergenic spacer (IGS) region ...the identification of microorganisms. The D1/D2 ... See full document
5
High Throughput Detection of Pathogenic Yeasts of the Genus Trichosporon
... the medically important genus ...to species-specific capture ...48 species-specific and group-specific capture probes designed in the D1/D2 region of ribosomal DNA, internal ... See full document
11
Typing of Histoplasma capsulatumIsolates Based on Nucleotide Sequence Variation in the Internal Transcribed Spacer Regions of rRNA Genes
... Valley regions in the United ...forms yeast colonies when grown in the laboratory on blood agar at ...(RFLP) analysis of mitochondrial DNA ... See full document
5
Internal transcribed spacer (its) sequence analysis of nuclear ribosomal dna (nrdna) in averrhoa l
... The sequence quality was checked using Sequence Scanner Software v1 (Applied ...Biosystems). Sequence alignment and required editing of the obtained sequences were carried out using Geneious Pro ... See full document
7
Genetic comparison of different human population groups using internal transcribed spacer sequence
... students to determine the relationship between groups of different populations. The aim of this study was to determine if the variation or polymorphic loci in ITS is sufficient to differentiate between different ... See full document
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