[PDF] Top 20 Identification of Sequences in Herpes Simplex Virus Type 1 ICP22 That Influence RNA Polymerase II Modification and Viral Late Gene Expression
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Identification of Sequences in Herpes Simplex Virus Type 1 ICP22 That Influence RNA Polymerase II Modification and Viral Late Gene Expression
... which ICP22 enhances viral gene expres- sion is not yet ...that ICP22 enhances L-gene ex- pression by activating the cell cycle regulator cdc2, which helps to recruit topoisomerase ... See full document
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Tyrosine phosphorylation of the herpes simplex virus type 1 regulatory protein ICP22 and a cellular protein which shares antigenic determinants with ICP22.
... a viral protein or it is a cellular protein whose expression or modification was induced by the viral ...The ICP22 protein was of particular interest since viruses which contain ... See full document
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Binding of ICP4, TATA-Binding Protein, and RNA Polymerase II to Herpes Simplex Virus Type 1 Immediate-Early, Early, and Late Promoters in Virus-Infected Cells
... the gene, inactivate the DNA-binding proper- ties of the protein, or that retain DNA-binding properties but delete the amino- and carboxyl-terminal activation re- ...the viral genome during infection is ... See full document
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Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene
... gC expression by immunoblotting (Fig. 4C). As is shown, ICP27 expression was readily apparent for the WT protein and all mutants tested, except ...verified expression of most of the mutant ICP27 ... See full document
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Herpes Simplex Virus Immediate-Early Protein ICP22 Triggers Loss of Serine 2-Phosphorylated RNA Polymerase II
... of RNA polymerase II (RNAP II) are regulated by phosphorylation of its carboxyl-terminal domain (CTD), with modification occurring primarily on serines 2 and 5 of the ...that ... See full document
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Herpes Simplex Virus Type 1 Immediate-Early Protein ICP22 Is Required for VICE Domain Formation during Productive Viral Infection
... whether ICP22 and Hsc70 might physi- cally ...of ICP22 (1) ...with ICP22-specific antisera indicated that the immu- noprecipitation was successful, as all of the FLAG-tagged ICP22 ... See full document
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Repression of host RNA polymerase II transcription by herpes simplex virus type 1.
... IE gene product ICP4 is an essential viral transactivating protein which is required for transcriptional activation of the DE and L genes ...essential viral protein that is required for DE and L ... See full document
10
ICP22 and the UL13 Protein Kinase Are both Required for Herpes Simplex Virus-Induced Modification of the Large Subunit of RNA Polymerase II
... Herpes simplex virus type 1 (HSV-1) infection alters the phosphorylation of the large subunit of RNA polymerase II (RNAP II), resulting in the ... See full document
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Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... protein, ICP22, a 420-residue protein encoded by the Us1 gene, conserved in the subfamily Alphaherpesvirinae of the family Her- ...that ICP22 plays important roles in viral replication and ... See full document
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Comprehensive Characterization of Interaction Complexes of Herpes Simplex Virus Type 1 ICP22, UL3, UL4, and UL20.5
... the ICP22 ORF at codon 90 (6). Both ICP22 and ...and ICP22 localizes in small, dense nuclear structures early in infection ...of viral DNA synthesis, these structures then shift to replication ... See full document
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Herpes simplex virus immediate-early protein ICP22 is required for viral modification of host RNA polymerase II and establishment of the normal viral transcription program.
... of ICP22 in RNAP II phos- phorylation must take into account our observation that 22/ n199 infection still causes significant alterations in the phos- phorylation of the RNAP II large ...another ... See full document
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Pocket Protein p130/Rb2 Is Required for Efficient Herpes Simplex Virus Type 1 Gene Expression and Viral Replication
... of 1⫻ PBS. Cells were pelleted through 1 ml of CS and then resuspended in 200 l of 1⫻ PBS with ...and viral DNA were extracted using the QIAamp DNA minikit (Qiagen) according to the ... See full document
12
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... wild-type virus-infected cells, empty capsids are only rarely enveloped (35), and a variety of viral mutants that synthesize capsids but fail to package DNA do not efficiently envelop the empty ... See full document
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Activation of human papillomavirus type 18 gene expression by herpes simplex virus type 1 viral transactivators and a phorbol ester.
... A plasmid containing the HPV-18 noncoding region placed upstream of the chloramphenicol acetyltransferase reporter gene was cotransfected with different herpes simplex virus type 1 HSV-1[r] ... See full document
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Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... in viral transport from the nucleus to the cell membrane (1, ...during viral infection (reviewed in references 27 and ...cell type-dependent syncytial phenotype (2, 36; discussed in reference ... See full document
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Identification and mapping of two polypeptides encoded within the herpes simplex virus type 1 thymidine kinase gene sequences.
... McGEOCH Medical Research Council Virology Unit, Glasgow, Gll 5JR Scotland mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contai[r] ... See full document
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ICP0 Gene Expression Is a Herpes Simplex Virus Type 1 Apoptotic Trigger
... ICP27 gene and cannot produce late viral proteins (59), also results in PARP and caspase-3 cleavage ...(Fig. 1, lane 7). In contrast, infection with d109, a recombinant virus devoid of ... See full document
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Gene expression of herpes simplex virus. II. UV radiological analysis of viral transcription units.
... By using host cells lacking excision repair activity, xeroderma pigmentosum XP fibroblasts, to prevent the repair of the input irradiated viral genome, we have been able to analyze the s[r] ... See full document
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The Initiator Element in a Herpes Simplex Virus Type 1 Late-Gene Promoter Enhances Activation by ICP4, Resulting in Abundant Late-Gene Expression
... explain late- gene expression during viral ...after viral replication, and differences in polyadenylation sites (53, 62, 76, ...in late genes play a role in temporal ...in ... See full document
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Herpes Simplex Virus Type 1 Infection Leads to Loss of Serine-2 Phosphorylation on the Carboxyl-Terminal Domain of RNA Polymerase II
... Herpes simplex virus type 1 (HSV-1) is a common human pathogen that is associated with mild to severe ...host RNA polymerase II (RNAP II) and ... See full document
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