[PDF] Top 20 Identification of toxigenic Clostridium difficile strains by using a toxin A gene specific probe
Has 10000 "Identification of toxigenic Clostridium difficile strains by using a toxin A gene specific probe" found on our website. Below are the top 20 most common "Identification of toxigenic Clostridium difficile strains by using a toxin A gene specific probe".
Identification of toxigenic Clostridium difficile strains by using a toxin A gene specific probe
... A 4.5-kilobase PstI fragment encoding part of the toxin A gene was isolated and used as a DNA probe in colony hybridization studies with 58 toxigenic and 17 nontoxigenic Clostridium diff[r] ... See full document
5
Multicenter Clinical Evaluation of the Portrait Toxigenic C difficile Assay for Detection of Toxigenic Clostridium difficile Strains in Clinical Stool Specimens
... lostridium difficile has emerged as a major nosocomial patho- gen and is a leading cause of antibiotic-associated diarrhea and pseudomembranous colitis ...C. difficile-associated disease; however, the ... See full document
5
Bacteriophage-Mediated Toxin Gene Regulation in Clostridium difficile
... in toxin gene regu- lation using a reporter fusion in ...experiments using Clostridium perfringens (27) and later in ...coli strains carrying either pACYC521 or ...tural ... See full document
9
Evaluation of a New Automated Homogeneous PCR Assay, GenomEra C difficile, for Rapid Detection of Toxigenic Clostridium difficile in Fecal Specimens
... of toxigenic C. difficile, being more sensitive than IA tests and EIAs and significantly faster than the standard meth- ods (24, 36, ...of toxigenic C. difficile among children (40, 41) and ... See full document
5
Premarket Evaluations of the IMDx C difficile for Abbott m2000 Assay and the BD Max Cdiff Assay
... Clostridium difficile-associated diarrhea is a well-recognized complication of antibiotic ...C. difficile has been difficult, as antigen assays are insensitive and culture-based methods require ... See full document
6
Real Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool
... of toxigenic C. difficile or its toxins (CDT) or colonoscopic or histopathologic findings demonstrat- ing pseudomembranous colitis ...C. difficile-specific antigen ...[EIA]), toxin ... See full document
7
Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
... of toxin that are below the detection limits of toxin ELISA or cytotoxin neutralization ...C. difficile spores in the hospital setting, because they test negative by conventional methods and are ... See full document
6
High Prevalence of Toxigenic and Nontoxigenic Clostridium difficile Strains in Malaysia
... of Clostridium difficile in Southeast Asia associated with a range of clinical ...and toxin A or B were performed on diarrheal stool specimens collected from patients in 2015 and ...of toxin genes. In ... See full document
7
Prevalence and Genetic Characterization of Toxin A Variant Strains of Clostridium difficile among Adults and Children with Diarrhea in France
... A-negative strains still remains unclear. In the hamster model of C. difficile disease, strain 8864 was pathogenic (7) but strains from serotype F (including strain 1470) were reported to be ... See full document
5
Yield of Stool Culture with Isolate Toxin Testing versus a Two Step Algorithm Including Stool Toxin Testing for Detection of Toxigenic Clostridium difficile
... its identification in 1978, Clostridium difficile has emerged as the predominant cause of antibiotic-associated co- litis and the leading cause of diarrhea in hospitalized patients ...(4). ... See full document
5
Identification of Toxin A Negative, Toxin B Positive Clostridium difficile by PCR
... discriminated toxin A1, toxin B1, toxin A2, toxin B1, and toxin A2, toxin B2 strains of ...C. difficile by PCR using two primer sets derived from the nonre- ... See full document
5
Rapid detection of toxigenic Clostridium difficile in fecal samples by magnetic immuno PCR assay
... Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by polymerase chain reaction.. Characterisation of Clostridium difficile strains by [r] ... See full document
5
Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification
... C. difficile could not be diagnosed by using only the TcdA detection kit (1, 3, 16, 17, ...C. difficile is a sensitive and specific method when cultured isolates are tested for TcdA and TcdB ... See full document
5
Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay
... C. difficile strains, 15 (15.3%) strains were positive for toxin genes, 12 ...(12.2%) strains had toxin B and A, two (2%) strains had only toxin A (A+B-) and one ... See full document
7
Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
... lostridium difficile infection (CDI) is a major cause of nosocomial diarrhea, leading to morbidity and mortality in hospitalized pa- tients ...and toxigenic strains of C. difficile. ... See full document
6
Molecular and Susceptibility Analysis of Toxigenic Clostridium difficile Obtained from Adult Patients Suspected of CDI in Trinidad
... Clostridium difficile is a Gram positive rod-shaped bacterium that produces two major toxins, A and ...out using specialized Enzyme-Linked Immunosorbent Assay (ELISA) kits; however these generally ... See full document
15
Correlation of Disease Severity with Fecal Toxin Levels in Patients with Clostridium difficile Associated Diarrhea and Distribution of PCR Ribotypes and Toxin Yields In Vitro of Corresponding Isolates
... feces using the selective medium ...by using ethanol preparations of spores from batch cultures ...C. difficile toxin yields in vitro (15, 16), nutrients present in the colonic lumen may also ... See full document
6
A Clostridium difficile Specific, Gel Forming Protein Required for Optimal Spore Germination
... ABSTRACT Clostridium difficile is a Gram-positive spore-forming obligate anaerobe that is a leading cause of antibiotic-associated diarrhea worldwide. In order for C. difficile to initiate infection, its ... See full document
17
High Throughput Analysis of Gene Essentiality and Sporulation in Clostridium difficile
... analyzed using a broth-based sporulation assay ...this gene in the 630 ⌬ erm spore ...followed using phase-contrast and fluorescence microscopy ...and using phase-contrast micros- copy ... See full document
13
Monoclonal antibodies specific for Clostridium difficile toxin B and their use in immunoassays
... difficile culture filtrate and Mono Q-purified fractions were incubated with these latex beads to bind the cytotoxin and then centrifuged to remove the beads, a drastic decrease of the t[r] ... See full document
7
Related subjects