[PDF] Top 20 Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants.

... In addition, survival rates determined from plating efficiencies of the various cell mutants after incubations at 39.5°C for the time periods corresponding 42 Downloaded from http://jvi.[r] ... See full document

... Effect of coincubating MNCs and anti-IFN sera on HSV infection in fibroblast monolayersa MNCs added Antisera added PFU recovered 103 % Plaque inhibition + + + None None Anti-IFN-a Anti-I[r] ... See full document

... noncompetitive inhibitor of nitric oxide synthetase substantially reduced the antiviral effect of activated RAW macrophages. S-nitroso-L-acetyl penicillamine (SNAP) is a donor of NO and was added to the media of infected ... See full document

... during virus rep- lication is the targeting of viral components to the appropriate cell compartment for viral assembly (9, 18, ...the virus and the glycoprotein ...the virus life cycle, ... See full document

... for 1 h at ...room temperature and then rinsed and replenished with serum-free ...untreated. Cell-associated HSV-1 genome copies were quantitated by ... See full document

... wild-type virus showed a decrease of about ...wild-type virus was more pronounced follow- ing exposure to increasing intensities of UV-C ...wild-type virus was likely due to an ... See full document

... These results demonstrate that immunization with replication-defective mutants of HSV-1 can efficiently elicit a T-cell- and B-cell-mediated immune response and that the response generat[r] ... See full document

... more sensitive than the TK mutagenesis, even though the difference between their repli- cation modes (episomal verse genomic) may also affect the outcomes of DNA replication fidelity (10, ... See full document

... Consistent with this model are the following observations: i certain late proteins e.g., VP154 can be expressed in the absence of functional VP175 this study, ii VP175 is required for ce[r] ... See full document

... In addition to cell lines which complemented ts mutants, two lines, U-27 and U-35, significantly inhibited plaque formation by wild-type virus, contained 30 and 100 copies of the ICP8 ge[r] ... See full document

... apoptosis, cell cycle, cellular senescence, DNA repair, autophagy, and innate im- mune control (21, ...a type of cell stress, p53’s effects can reduce replication of some viruses, and, ... See full document

... the inhibition of HSV-1 replication was not caused by an altered transcription of the viral genes required for DNA ...DNA replication, whereas the transcription of late genes requires DNA ... See full document

... 704 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Biochemical transformation assays of herpes simplex virus type 1 temperaturesensitive ts mutants distinguished three[r] ... See full document

... reduced virus titer, whereas ⌬E4/5 mutant had a minimal effect ...viral replication correlated with the reduced expression of ICP0 and UL42 ...HSV-1 replication. (A) Structures of ... See full document

... We previously reported that during very active viral tran- scription in wild-type HSV-1-infected cells, RNAP II under- goes proteasomal degradation (5). It is the serine 2-phosphor- ylated form of the RNAP ... See full document

... apoptosis pathway depending on the specific cell type. It is our current hypothesis that HSV-2 ICP10 (UL39) gene expression leads to the same initial signaling event in all cell types, yet results in ... See full document

... 48-55 0022-538X/94/$04.00 + 0 Copyright C 1994, American Society for Microbiology ICP34.5 Mutants of Herpes Simplex Virus Type 1 Strain 17syn+ Are Attenuated for Neurovirulence in Mice a[r] ... See full document

... the cell against viral infection, can increase the size and number of ND10 and can result in inhibition of viral infection supports this model (5, 28, 44, 45, 51, ...ICP0-null mutants, which are ... See full document

... Mutants representing all seven complementation groups failed to grow in either of these two transformed cell lines at the nonpermissive temperature, as measured by plaque formation on th[r] ... See full document

... Fifteen to fifty percent of wild-type levels of viral DNA are synthesized at the nonpermissive temperature in ts48- and ts303-infected cells, indicating that immediate-early and early ge[r] ... See full document